Escherichia coli K-12 substr. MG1655 Enzyme: phosphoethanolamine transferase EptC

Gene: eptC Accession Numbers: EG11914 (EcoCyc), b3955, ECK3946

Synonyms: yijP

Regulation Summary Diagram: ?

Regulation summary diagram for eptC

EptC is required for the incorporation of phosphoethanolamine (P-EtN) onto the phosphorylated heptose I residue of E.coli K-12 lipopolysaccharide (LPS) [Klein13]. P-EtN modifications are absent on the LPS of E.coli grown under phosphate-rich conditions but are enriched in the LPS from growth under phosphate-limiting conditions [Klein13].

eptC deleted strains have severe growth defects in the presence of SDS (0.25% - 1%) and are more sensitive to Zn2+ than the wild-type [Klein13].

Transcription of an eptC-lacZ fusion is reduced 4-5 fold in a phoR deletion mutant and reduced 2-fold in a basR deletion mutant. Two putative PhoB binding sites are located in the promoter region of eptC [Klein13].

LPS from an E.coli strain lacking all three ept genes (eptA, eptB and eptC) does not contain any P-EtN modifications [Klein13].

EptC (formerly YijP) has similarity to YijP of E. coli K1, which is required for that strain to invade brain microvascular endothelial cells and thereby cross the blood-brain barrier [Wang99f]. Reviews discuss the role of YijP in meningitis caused by pathogenic E. coli [Huang00a, Huang01a].

EptC was identified as a component of a complex in the inner membrane together with an unidentified 36 kDa protein [Stenberg05].

Locations: inner membrane

Map Position: [4,146,555 <- 4,148,288] (89.37 centisomes, 322°)
Length: 1734 bp / 577 aa

Molecular Weight of Polypeptide: 66.61 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012947 , EchoBASE:EB1858 , EcoGene:EG11914 , EcoliWiki:b3955 , ModBase:P32678 , OU-Microarray:b3955 , PortEco:yijP , Pride:P0CB39 , Protein Model Portal:P0CB39 , RefSeq:NP_418390 , RegulonDB:EG11914 , String:511145.b3955 , UniProt:P0CB39

Relationship Links: InterPro:IN-FAMILY:IPR000917 , InterPro:IN-FAMILY:IPR012549 , InterPro:IN-FAMILY:IPR017849 , InterPro:IN-FAMILY:IPR017850 , Pfam:IN-FAMILY:PF00884 , Pfam:IN-FAMILY:PF08019

In Paralogous Gene Group: 204 (5 members) , 503 (3 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0009244 - lipopolysaccharide core region biosynthetic process Inferred from experiment Inferred by computational analysis [UniProtGOA12, Klein13]
GO:0006629 - lipid metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01a]
GO:0009103 - lipopolysaccharide biosynthetic process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0016776 - phosphotransferase activity, phosphate group as acceptor Inferred from experiment [Klein13]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0008484 - sulfuric ester hydrolase activity Inferred by computational analysis [GOA01a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05, Stenberg05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane

Essentiality data for eptC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Enzymatic reaction of: phosphoethanolamine transferase EptC

an L-1-phosphatidylethanolamine[periplasmic space] + lipid A-core[periplasmic space] <=> a 1,2-diacyl-sn-glycerol[periplasmic space] + lipid A-core-phosphoethanolamine (hep II)[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

Sequence Features

Protein sequence of phosphoethanolamine transferase EptC with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 17 -> 37
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 44 -> 64
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 69 -> 89
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 119 -> 139
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 154 -> 174
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b3955 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11914; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Huang00a: Huang SH, Stins MF, Kim KS (2000). "Bacterial penetration across the blood-brain barrier during the development of neonatal meningitis." Microbes Infect 2(10);1237-44. PMID: 11008113

Huang01a: Huang SH, Jong AY (2001). "Cellular mechanisms of microbial proteins contributing to invasion of the blood-brain barrier." Cell Microbiol 3(5);277-87. PMID: 11298651

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Klein13: Klein G, Muller-Loennies S, Lindner B, Kobylak N, Brade H, Raina S (2013). "Molecular and structural basis of inner core lipopolysaccharide alterations in Escherichia coli: incorporation of glucuronic acid and phosphoethanolamine in the heptose region." J Biol Chem 288(12);8111-27. PMID: 23372159

Stenberg05: Stenberg F, Chovanec P, Maslen SL, Robinson CV, Ilag LL, von Heijne G, Daley DO (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280(41);34409-19. PMID: 16079137

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Wang99f: Wang Y, Huang SH, Wass CA, Stins MF, Kim KS (1999). "The gene locus yijP contributes to Escherichia coli K1 invasion of brain microvascular endothelial cells." Infect Immun 67(9);4751-6. PMID: 10456927

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Other References Related to Gene Regulation

Raivio13: Raivio TL, Leblanc SK, Price NL (2013). "The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity." J Bacteriol 195(12);2755-67. PMID: 23564175

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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