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Escherichia coli K-12 substr. MG1655 Enzyme: tRNA-dihydrouridine synthase A



Gene: dusA Accession Numbers: EG11932 (EcoCyc), b4049, ECK4041

Synonyms: yjbN

Regulation Summary Diagram: ?

Summary:
DusA accounts for about half of the 5,6-dihydrouridine modification observed in wild-type cellular tRNA; DusB and DusC together account for the other half. DusA is solely responsible for the 5,6-dihydrouridine modification observed in tRNA2fMet [Bishop02].

A dusA dusB dusC triple mutant exhibits a complete lack of 5,6-dihydrouridine modification in cellular tRNA, whereas each single mutant exhibits a partial reduction, compared to wild type [Bishop02]. Amino acids that are required for DusA activity have been identified [Savage06].

DusA: "dihydrouridine synthase A"

Locations: cytosol

Map Position: [4,259,692 -> 4,260,729] (91.81 centisomes)
Length: 1038 bp / 345 aa

Molecular Weight of Polypeptide: 38.468 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013258 , EchoBASE:EB1876 , EcoGene:EG11932 , EcoliWiki:b4049 , ModBase:P32695 , OU-Microarray:b4049 , PortEco:dusA , PR:PRO_000022490 , Pride:P32695 , Protein Model Portal:P32695 , RefSeq:NP_418473 , RegulonDB:EG11932 , SMR:P32695 , String:511145.b4049 , UniProt:P32695

Relationship Links: InterPro:IN-FAMILY:IPR001269 , InterPro:IN-FAMILY:IPR004653 , InterPro:IN-FAMILY:IPR013785 , InterPro:IN-FAMILY:IPR018517 , Panther:IN-FAMILY:PTHR11082 , Pfam:IN-FAMILY:PF01207 , Prosite:IN-FAMILY:PS01136

In Paralogous Gene Group: 511 (3 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0002943 - tRNA dihydrouridine synthesis Inferred by computational analysis Inferred from experiment [Bishop02, GOA01a]
GO:0008033 - tRNA processing Inferred by computational analysis [UniProtGOA11a]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0017150 - tRNA dihydrouridine synthase activity Inferred from experiment Inferred by computational analysis [GOA01a, Bishop02]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0050660 - flavin adenine dinucleotide binding Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, Zhang07]

MultiFun Terms: information transfer RNA related RNA modification

Essentiality data for dusA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 22-Mar-2012 by Keseler I , SRI International


Enzymatic reaction of: tRNA-dihydrouridine synthase

EC Number: 1.1.1.-

a 5,6-dihydrouridine in tRNA + NADP+ <=> a uridine in tRNA + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the opposite direction.


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b4049 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11932; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bishop02: Bishop AC, Xu J, Johnson RC, Schimmel P, de Crecy-Lagard V (2002). "Identification of the tRNA-dihydrouridine synthase family." J Biol Chem 277(28);25090-5. PMID: 11983710

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Savage06: Savage DF, de Crecy-Lagard V, Bishop AC (2006). "Molecular determinants of dihydrouridine synthase activity." FEBS Lett 580(22);5198-202. PMID: 16962594

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC14B.