Escherichia coli K-12 substr. MG1655 Polypeptide: phage shock protein G

Gene: pspG Accession Numbers: EG11933 (EcoCyc), b4050, ECK4042

Synonyms: yjbO

Regulation Summary Diagram: ?

Regulation summary diagram for pspG

The PspG protein is predicted to be an inner membrane protein. PspG is conserved in all bacteria that contain the psp operon. The PspG protein appears to affect motility [Lloyd04].

Expression of the pspG gene, like the phage shock operon pspABCDE, is upregulated by pIV secretin stress. Transcription by sigma54-containing RNA polymerase may be activated by PspF, and PspA negatively regulates pspG expression. pspG thus belongs to the PspF regulon [Lloyd04].

PspG self associates and interacts with PspC in vivo [Jovanovic10]

Locations: inner membrane

Map Position: [4,260,863 -> 4,261,105] (91.84 centisomes, 331°)
Length: 243 bp / 80 aa

Molecular Weight of Polypeptide: 9.023 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013260 , EchoBASE:EB1877 , EcoGene:EG11933 , EcoliWiki:b4050 , OU-Microarray:b4050 , PortEco:pspG , Pride:P32696 , Protein Model Portal:P32696 , RefSeq:NP_418474 , RegulonDB:EG11933 , String:511145.b4050 , UniProt:P32696

Relationship Links: InterPro:IN-FAMILY:IPR014318 , Pfam:IN-FAMILY:PF09583

Genetic Regulation Schematic: ?

Genetic regulation schematic for pspG

GO Terms:

Biological Process: GO:0009271 - phage shock Inferred from experiment [Lloyd04]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Lloyd04]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell processes defense/survival

Essentiality data for pspG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Sequence Features

Protein sequence of phage shock protein G with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 5 -> 25
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 41 -> 61
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b4050 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11933; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Jovanovic10: Jovanovic G, Engl C, Mayhew AJ, Burrows PC, Buck M (2010). "Properties of the phage shock protein (Psp) regulatory complex that govern signal transduction and induction of the Psp response in Escherichia coli." Microbiology. PMID: 20595257

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lloyd04: Lloyd LJ, Jones SE, Jovanovic G, Gyaneshwar P, Rolfe MD, Thompson A, Hinton JC, Buck M (2004). "Identification of a new member of the phage shock protein response in Escherichia coli, the phage shock protein G (PspG)." J Biol Chem 279(53):55707-14. PMID: 15485810

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc14.