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Escherichia coli K-12 substr. MG1655 Polypeptide: putative type-1 fimbrial protein



Gene: yehA Accession Numbers: EG11987 (EcoCyc), b2108, ECK2101

Regulation Summary Diagram: ?

Summary:
yehABCD is a putative chaperone-usher fimbrial operon in E.coli K-12 [Korea10]. The operon is cryptic under normal laboratory conditions but when constitutive expression is induced in a strain that lacks the type 1 fimbrial complex the yeh operon promotes biofilm formation in minimal media on a variety of abiotic surfaces [Korea10]. Expression of the yeh operon is regulated by the global regulators H-NS and CRP cAMP [Korea10].

YehA shares a low sequence similarity to members of the Autotransporter (AT) Family [Zhai02].

Citations: [Korea11]

Locations: pilus

Map Position: [2,185,402 <- 2,186,436] (47.1 centisomes)
Length: 1035 bp / 344 aa

Molecular Weight of Polypeptide: 36.885 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006972 , EchoBASE:EB1930 , EcoGene:EG11987 , EcoliWiki:b2108 , OU-Microarray:b2108 , PortEco:yehA , Protein Model Portal:P33340 , RefSeq:NP_416611 , RegulonDB:EG11987 , SMR:P33340 , String:511145.b2108 , UniProt:P33340

Relationship Links: InterPro:IN-FAMILY:IPR000259 , InterPro:IN-FAMILY:IPR008966 , InterPro:IN-FAMILY:IPR016959 , Pfam:IN-FAMILY:PF00419

GO Terms:

Biological Process: GO:0043709 - cell adhesion involved in single-species biofilm formation Inferred from experiment [Korea10]
GO:0007155 - cell adhesion Inferred by computational analysis [GOA01]
Cellular Component: GO:0009289 - pilus Inferred by computational analysis [GOA01, Korea10]

MultiFun Terms: cell structure pilus

Essentiality data for yehA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 14-Apr-2010 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 20
[UniProt10a]
UniProt: Non-Experimental Qualifier: potential;
Chain 21 -> 344
[UniProt09]
UniProt: Uncharacterized protein yehA;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2108 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11987; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Korea10: Korea CG, Badouraly R, Prevost MC, Ghigo JM, Beloin C (2010). "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities." Environ Microbiol 12(7):1957-77. PMID: 20345943

Korea11: Korea CG, Ghigo JM, Beloin C (2011). "The sweet connection: Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli fimbriae form a versatile arsenal of sugar-binding lectins potentially involved in surface-colonisation and tissue tropism." Bioessays 33(4);300-11. PMID: 21344461

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

Zhai02: Zhai Y, Saier MH (2002). "The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes." Protein Sci 11(9);2196-207. PMID: 12192075


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13A.