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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted kinase



Gene: yeiI Accession Numbers: EG12028 (EcoCyc), b2160, ECK2153

Regulation Summary Diagram: ?

Summary:
No information about this protein was found by a literature search conducted on June 15, 2007.

Locations: cytosol

Map Position: [2,249,722 -> 2,250,810] (48.49 centisomes)
Length: 1089 bp / 362 aa

Molecular Weight of Polypeptide: 39.71 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007149 , DIP:DIP-11921N , EchoBASE:EB1963 , EcoGene:EG12028 , EcoliWiki:b2160 , ModBase:P33020 , OU-Microarray:b2160 , PortEco:yeiI , Protein Model Portal:P33020 , RefSeq:NP_416665 , RegulonDB:EG12028 , SMR:P33020 , String:511145.b2160 , UniProt:P33020

Relationship Links: InterPro:IN-FAMILY:IPR002173 , InterPro:IN-FAMILY:IPR011611 , InterPro:IN-FAMILY:IPR011991 , Pfam:IN-FAMILY:PF00294 , Pfam:IN-FAMILY:PF13412 , Prosite:IN-FAMILY:PS00583 , Prosite:IN-FAMILY:PS00584

In Paralogous Gene Group: 340 (11 members)

GO Terms:

Biological Process: GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016773 - phosphotransferase activity, alcohol group as acceptor Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

Gene Class: ORFs

Essentiality data for yeiI knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 15-Jun-2007 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Sequence-Conflict 335 -> 362
[Richterich93, UniProt10]
Alternate sequence: CAAISRASGSLNNPTLSADNALSLVPMV → ARQFRAPAAA; UniProt: (in Ref. 1; AAA60512);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2160 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12028; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Richterich93: Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. (1993). "Automated multiplex sequencing of the E.coli genome." Data submission to EMBL/GenBank/DDBJ databases on 1993-10.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC13A.