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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted dehydrogenase, NAD-dependent



Gene: yeiQ Accession Numbers: EG12036 (EcoCyc), b2172, ECK2166

Regulation Summary Diagram: ?

Summary:
No information about this protein was found by a literature search conducted on January 23, 2014.

Locations: cytosol

Map Position: [2,264,267 -> 2,265,733] (48.8 centisomes)
Length: 1467 bp / 488 aa

Molecular Weight of Polypeptide: 54.032 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007191 , EchoBASE:EB1971 , EcoGene:EG12036 , EcoliWiki:b2172 , ModBase:P33029 , OU-Microarray:b2172 , PortEco:yeiQ , Pride:P33029 , Protein Model Portal:P33029 , RefSeq:NP_416677 , RegulonDB:EG12036 , SMR:P33029 , String:511145.b2172 , UniProt:P33029

Relationship Links: InterPro:IN-FAMILY:IPR000669 , InterPro:IN-FAMILY:IPR008927 , InterPro:IN-FAMILY:IPR013118 , InterPro:IN-FAMILY:IPR013131 , InterPro:IN-FAMILY:IPR013328 , InterPro:IN-FAMILY:IPR016040 , InterPro:IN-FAMILY:IPR023027 , Pfam:IN-FAMILY:PF01232 , Pfam:IN-FAMILY:PF08125 , Prints:IN-FAMILY:PR00084 , Prosite:IN-FAMILY:PS00974

In Paralogous Gene Group: 306 (5 members)

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
GO:0019594 - mannitol metabolic process Inferred by computational analysis [GOA01]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016616 - oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred by computational analysis [GOA01]
GO:0050662 - coenzyme binding Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

Gene Class: ORFs

Essentiality data for yeiQ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 23-Jan-2014 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 27 -> 38
[UniProt10a]
UniProt: NAD; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 48
[Itoh96, UniProt10]
Alternate sequence: L → R; UniProt: (in Ref. 2);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2172 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12036; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Itoh96: Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3(6);379-92. PMID: 9097040

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC13A.