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discounted EARLY registration ends Dec 31, 2014
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for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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Escherichia coli K-12 substr. MG1655 Polypeptide: conserved protein



Gene: ppdA Accession Numbers: EG12081 (EcoCyc), b2826, ECK2822

Regulation Summary Diagram: ?

Summary:
A ppdAB mutant does not exhibit defects in chemotaxis or flagellar motility [Park01]. Overproduction of PpdAB does not result in defects in chemotaxis or flagellar motility [Park01].

PpdA: "prepilin peptidase dependent"

Regulation has been described [Park01]. Transcription is sigmaF (FliA) regulated [Park01]. Transcription is regulated by FlhDC [Park01].

Locations: pilus

Map Position: [2,961,729 <- 2,962,199] (63.83 centisomes)
Length: 471 bp / 156 aa

Molecular Weight of Polypeptide: 17.865 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009266 , EchoBASE:EB2006 , EcoGene:EG12081 , EcoliWiki:b2826 , OU-Microarray:b2826 , PortEco:ppdA , Protein Model Portal:P33554 , RefSeq:NP_417303 , RegulonDB:EG12081 , SMR:P33554 , String:511145.b2826 , UniProt:P33554

Relationship Links: InterPro:IN-FAMILY:IPR012902 , Pfam:IN-FAMILY:PF07963 , Prosite:IN-FAMILY:PS00409

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0009101 - glycoprotein biosynthetic process
Cellular Component: GO:0009289 - pilus

MultiFun Terms: cell structure pilus

Essentiality data for ppdA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Propeptide 1 -> 6
[UniProt10]
UniProt: Non-Experimental Qualifier: by similarity;
Sequence-Conflict 5
[Finch86, UniProt10a]
Alternate sequence: R → A; UniProt: (in Ref. 1);
Methylation-Modification 7
[UniProt10]
UniProt: N-methyltyrosine; Non-Experimental Qualifier: by similarity;
Chain 7 -> 156
[UniProt09]
UniProt: Prepilin peptidase-dependent protein A;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b2826 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12081; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Finch86: Finch PW, Wilson RE, Brown K, Hickson ID, Tomkinson AE, Emmerson PT (1986). "Complete nucleotide sequence of the Escherichia coli recC gene and of the thyA-recC intergenic region." Nucleic Acids Res 14(11);4437-51. PMID: 3520484

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Park01: Park K, Choi S, Ko M, Park C (2001). "Novel sigmaF-dependent genes of Escherichia coli found using a specified promoter consensus." FEMS Microbiol Lett 2001;202(2);243-50. PMID: 11520622

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14A.