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Escherichia coli K-12 substr. MG1655 Polypeptide: glyceraldehyde 3-phosphate dehydrogenase C, interrupted



Gene: gapC_2 Accession Numbers: EG12103 (EcoCyc), b1416, ECK1409

Synonyms: gapC

Regulation Summary Diagram: ?

Gene Citations: [Salmon03]

Locations: cytosol

Map Position: [1,487,737 <- 1,487,988] (32.07 centisomes)
Length: 252 bp / 83 aa

Molecular Weight of Polypeptide: 9.226 kD (from nucleotide sequence)

Unification Links: EcoliWiki:b1416 , OU-Microarray:b1416 , PortEco:gapC_2 , RegulonDB:EG12103

Relationship Links: EcoGene:RELATED-TO:EG12103 , EcoO157Cyc:Homolog:GAPC , EcoO157Cyc:Homolog:GAPC-MONOMER , ModBase:RELATED-TO:P33898 , Swiss-Model:RELATED-TO:P33898 , UniProt:RELATED-TO:P33898

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006006 - glucose metabolic process Inferred by computational analysis [GOA01a]
GO:0006096 - glycolytic process Inferred by computational analysis [UniProtGOA12, UniProtGOA11a]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0004365 - glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred by computational analysis [GOA01]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016620 - oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred by computational analysis [GOA01a]
GO:0050661 - NADP binding Inferred by computational analysis [GOA01a]
GO:0051287 - NAD binding Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]

Gene Class: Pseudo-Genes Interrupted-Genes

Essentiality data for gapC_2 knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Sequence Features

Feature Class Location Citations Comment
Amino-Acid-Sites-That-Bind 34
[UniProt10a]
UniProt: NAD; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 39
[Krawetz90, Krawetz86, UniProt10]
Alternate sequence: S → Y; UniProt: (in Ref. 8 and 9);
Amino-Acid-Sites-That-Bind 78
[UniProt10a]
UniProt: NAD; via carbonyl oxygen; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
5/7/1998 (pkarp) Merged genes G6734/gapC and EG12103/gapC


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Krawetz86: Krawetz SA, Connor W, Cannon PD, Dixon GH (1986). "A vector-primer-cloner-sequencer plasmid for the construction of cDNA libraries: evidence for a rat glyceraldehyde-3-phosphate dehydrogenase-like mRNA and a ferritin mRNA within testis." DNA 5(5);427-35. PMID: 3780374

Krawetz90: Krawetz S.A. (1990). Data submission to EMBL/GenBank/DDBJ databases on 1990-10.

Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, biocyc13.