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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted cytochrome C peroxidase



Gene: yhjA Accession Numbers: EG12244 (EcoCyc), b3518, ECK3503

Regulation Summary Diagram: ?

Summary:
A yhjA mutant shows increased sensitivity to exogenous peroxides [Partridge07].

Expression of yhjA is increased under anaerobic conditions and is dependent on FNR and OxyR [Partridge07].
By making use of micro arrays analysis, Constantinidou et al [Constantinidou06] concluded that FNR activates yhjA gene expression. They also identified a putative FNR binding site upstream of the gene, but the sequence was not shown.

Locations: periplasmic space

Map Position: [3,665,814 <- 3,667,211] (79.01 centisomes)
Length: 1398 bp / 465 aa

Molecular Weight of Polypeptide: 51.571 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011493 , EchoBASE:EB2155 , EcoGene:EG12244 , EcoliWiki:b3518 , ModBase:P37197 , OU-Microarray:b3518 , PortEco:yhjA , Pride:P37197 , Protein Model Portal:P37197 , RefSeq:NP_417975 , RegulonDB:EG12244 , SMR:P37197 , String:511145.b3518 , UniProt:P37197

Relationship Links: InterPro:IN-FAMILY:IPR003088 , InterPro:IN-FAMILY:IPR004852 , InterPro:IN-FAMILY:IPR009056 , InterPro:IN-FAMILY:IPR025992 , Pfam:IN-FAMILY:PF00034 , Pfam:IN-FAMILY:PF03150 , Pfam:IN-FAMILY:PF14376 , Prosite:IN-FAMILY:PS51007

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0042542 - response to hydrogen peroxide Inferred from experiment [Partridge07]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0004130 - cytochrome-c peroxidase activity Inferred by computational analysis [GOA01a]
GO:0004601 - peroxidase activity Inferred by computational analysis [UniProtGOA11]
GO:0005506 - iron ion binding Inferred by computational analysis [GOA01]
GO:0009055 - electron carrier activity Inferred by computational analysis [GOA01]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0020037 - heme binding Inferred by computational analysis [GOA01]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes protection

Essentiality data for yhjA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 02-May-2007 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Amino-Acid-Sites-That-Bind 59
[UniProt10a]
UniProt: Heme 1 (covalent); Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 62
[UniProt10a]
UniProt: Heme 1 (covalent); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 63
[UniProt10a]
UniProt: Iron (heme 1 axial ligand); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 195
[UniProt10a]
UniProt: Iron (heme 1 axial ligand); Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 207
[UniProt10a]
UniProt: Heme 2 (covalent); Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 210
[UniProt10a]
UniProt: Heme 2 (covalent); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 211
[UniProt10a]
UniProt: Iron (heme 2 axial ligand); Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 351
[UniProt10a]
UniProt: Heme 3 (covalent); Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 354
[UniProt10a]
UniProt: Heme 3 (covalent); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 355
[UniProt10a]
UniProt: Iron (heme 3 axial ligand); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 415
[UniProt10a]
UniProt: Iron (heme 2 axial ligand); Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 429
[UniProt10a]
UniProt: Iron (heme 3 axial ligand); Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3518 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12244; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Constantinidou06: Constantinidou C, Hobman JL, Griffiths L, Patel MD, Penn CW, Cole JA, Overton TW (2006). "A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL, and NarQP as Escherichia coli K12 adapts from aerobic to anaerobic growth." J Biol Chem 281(8);4802-15. PMID: 16377617

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Partridge07: Partridge JD, Poole RK, Green J (2007). "The Escherichia coli yhjA gene, encoding a predicted cytochrome c peroxidase, is regulated by FNR and OxyR." Microbiology 153(Pt 5);1499-509. PMID: 17464064

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, BIOCYC14B.