|Gene:||bcsZ||Accession Numbers: EG12258 (EcoCyc), b3531, ECK3516|
Synonyms: bcsC, yhjM
The bcsZ gene encodes an endo-1,4-D-glucanase which belongs to glycosyl hydrolase family 8 [Park99a].
BcsZ has a predicted signal sequence [Park99a]; a computational prediction that BcsZ is a lipoprotein is thought to be a probable false positive [Gonnet04]. In cell fractionation assays, the enzymatic activity appeared to be primarily extracellular [Park99a], although the publication reported no positive controls.
Crystal structures of apo-BcsZ and that of a catalytically inactive mutant bound to the substrate cellopentaose have been solved [Mazur11].
BcsZ is encoded in a predicted operon together with bcsA, bcsB, and bcsC. In other organisms, these genes are involved in cellulose biosynthesis, a characteristic of the rdar morphotype. However, the K-12 laboratory strain of E. coli does not show a rdar morphotype and does not produce cellulose [Zogaj01]. In E. coli K-12 strains such as W3110 and MG1655, a "domesticating SNP" consists of a point mutation that introduces a stop codon after the first 5 amino acids of the wild-type bcsQ ORF. The bcsQ ORF shown in EcoCyc begins just downstream of this SNP and appears to be non-functional. This domesticating SNP lowers expression of both bcsQ and the downstream bcsA gene. After repairing the SNP, the resulting "de-domesticated" W3110 derivative strain produces cellulose and has dramatically altered biofilm morphology [Serra13].
BcsZ: "bacterial cellulose synthesis"
Locations: extracellular space
|Map Position: [3,687,178 <- 3,688,284] (79.47 centisomes, 286°)||Length: 1107 bp / 368 aa|
Molecular Weight of Polypeptide: 41.7 kD (from nucleotide sequence), 40 kD (experimental) [Park99a ]
Unification Links: ASAP:ABE-0011541 , EchoBASE:EB2167 , EcoGene:EG12258 , EcoliWiki:b3531 , ModBase:P37651 , OU-Microarray:b3531 , PortEco:bcsZ , PR:PRO_000022205 , Pride:P37651 , Protein Model Portal:P37651 , RefSeq:NP_417988 , RegulonDB:EG12258 , SMR:P37651 , String:511145.b3531 , UniProt:P37651
Relationship Links: CAZy:IN-FAMILY:GH8 , InterPro:IN-FAMILY:IPR002037 , InterPro:IN-FAMILY:IPR008928 , InterPro:IN-FAMILY:IPR012341 , InterPro:IN-FAMILY:IPR019834 , PDB:Structure:3QXF , PDB:Structure:3QXQ , Pfam:IN-FAMILY:PF01270 , Prints:IN-FAMILY:PR00735 , Prosite:IN-FAMILY:PS00812
|Biological Process:||GO:0000272 - polysaccharide catabolic process
GO:0005975 - carbohydrate metabolic process [UniProtGOA11a, GOA01a]
GO:0008152 - metabolic process [UniProtGOA11a]
GO:0030245 - cellulose catabolic process [UniProtGOA11a]
|Molecular Function:||GO:0008810 - cellulase activity
GO:0003824 - catalytic activity [GOA01a]
GO:0004553 - hydrolase activity, hydrolyzing O-glycosyl compounds [GOA01a]
GO:0016787 - hydrolase activity [UniProtGOA11a]
GO:0016798 - hydrolase activity, acting on glycosyl bonds [UniProtGOA11a]
|Cellular Component:||GO:0005576 - extracellular region [UniProtGOA11, UniProtGOA11a, DiazMejia09, Park99a]|
|MultiFun Terms:||metabolism → degradation of macromolecules → polysaccharides|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB enriched||Yes||37||Aerobic||6.95||Yes [Gerdes03, Comment 1]|
|LB Lennox||Yes||37||Aerobic||7||Yes [Baba06, Comment 2]|
|M9 medium with 1% glycerol||Yes||37||Aerobic||7.2||0.35||Yes [Joyce06, Comment 3]|
|MOPS medium with 0.4% glucose||Yes||37||Aerobic||7.2||0.22||Yes [Baba06, Comment 2]|
Enzymatic reaction of: endo-1,4-D-glucanase
Synonyms: CMCase, β-1,4-D-glucan glucanohydrolase
EC Number: 220.127.116.11
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is physiologically favored in the direction shown.
Enzyme activity was assayed using carboxymethyl cellulose (CMC) as a substrate [Park99a].
T(opt): 40 °C [Park99a]
pH(opt): 7 [Park99a]
|Signal-Sequence||1 -> 21|
|Chain||22 -> 368|
Gene common name added 21-July-99. BcsC gene identified as an endo-1,4-D-glucanase, EC# 18.104.22.168. Ref:  Mol Gen Genet 1999 261:236-41. Park YW and Yun HD.
10/20/97 Gene b3531 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12258; confirmed by SwissProt match.
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
Blattner97: Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997). "The complete genome sequence of Escherichia coli K-12." Science 277(5331);1453-74. PMID: 9278503
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938
Gonnet04: Gonnet P, Rudd KE, Lisacek F (2004). "Fine-tuning the prediction of sequences cleaved by signal peptidase II: a curated set of proven and predicted lipoproteins of Escherichia coli K-12." Proteomics 4(6);1597-613. PMID: 15174130
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Omadjela13: Omadjela O, Narahari A, Strumillo J, Melida H, Mazur O, Bulone V, Zimmer J (2013). "BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis." Proc Natl Acad Sci U S A 110(44);17856-61. PMID: 24127606
Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220
Sofia94: Sofia HJ, Burland V, Daniels DL, Plunkett G, Blattner FR (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 1994;22(13);2576-86. PMID: 8041620
Zogaj01: Zogaj X, Nimtz M, Rohde M, Bokranz W, Romling U (2001). "The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix." Mol Microbiol 39(6);1452-63. PMID: 11260463
Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615
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