Escherichia coli K-12 substr. MG1655 Polypeptide: outer membrane protein YiaT

Gene: yiaT Accession Numbers: EG12288 (EcoCyc), b3584, ECK3573

Regulation Summary Diagram: ?

Regulation summary diagram for yiaT

YiaT is a putative outer membrane protein [Zhai02]. YiaT is predicted to contain 10 transmembrane β strands and 5 outer-exposed loops. C-terminal truncated YiaT has been used as a membrane anchor for the surface display of selected proteins [Han15].

Locations: outer membrane

Map Position: [3,749,151 <- 3,749,891] (80.81 centisomes, 291°)
Length: 741 bp / 246 aa

Molecular Weight of Polypeptide: 27.414 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011704 , EchoBASE:EB2196 , EcoGene:EG12288 , EcoliWiki:b3584 , OU-Microarray:b3584 , PortEco:yiaT , Protein Model Portal:P37681 , RefSeq:NP_418041 , RegulonDB:EG12288 , String:511145.b3584 , UniProt:P37681

Relationship Links: InterPro:IN-FAMILY:IPR010583 , Pfam:IN-FAMILY:PF06629

In Paralogous Gene Group: 540 (2 members)

GO Terms:

Cellular Component: GO:0009279 - cell outer membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, Han15, DiazMejia09]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]

Gene Class: ORFs

Essentiality data for yiaT knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Curated 02-Mar-2006 by Keseler I , SRI International
Last-Curated ? 19-Apr-2015 by Mackie A , Macquarie University

Sequence Features

Protein sequence of outer membrane protein YiaT with features indicated

Feature Class Location Common Name Citations Comment
Signal-Sequence 1 -> 21  
UniProt: Non-Experimental Qualifier: potential;
Chain 22 -> 246  
UniProt: Putative outer membrane protein yiaT;
Protein-Structure-Region 53 -> 61 L1
predicted extracellular loop
Protein-Structure-Region 83 -> 106 L2
predicted extracellular loop
Protein-Structure-Region 129 -> 136 L3
predicted extracellular loop
Protein-Structure-Region 163 -> 192 L4
predicted extracellular loop
Protein-Structure-Region 218 -> 235 L5
predicted extracellular loop

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b3584 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12288; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Han15: Han MJ, Lee SH (2015). "An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT." FEMS Microbiol Lett 362(1);1-7. PMID: 25790485

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Zhai02: Zhai Y, Saier MH (2002). "The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes." Protein Sci 11(9);2196-207. PMID: 12192075

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc13.