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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted fimbrial-like adhesin protein



Gene: yadK Accession Numbers: EG12325 (EcoCyc), b0136, ECK0135

Regulation Summary Diagram: ?

Summary:
yadCKLMhtrEecpDyadN is a putative chaperone-usher fimbrial operon in E.coli K-12 [Korea10]. The operon is cryptic under normal laboratory conditions but when constitutive expression is induced in a strain that lacks the type 1 fimbrial complex the yad operon promotes biofilm formation in minimal media on a variety of abiotic surfaces and produces surface fimbrial structures that can be observed microscopically [Korea10]. Expression of the yad operon is negatively regulated by H-NS [Korea10].

Citations: [Korea11]

Locations: pilus

Map Position: [151,003 <- 151,599] (3.25 centisomes)
Length: 597 bp / 198 aa

Molecular Weight of Polypeptide: 21.112 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000472 , EchoBASE:EB2229 , EcoGene:EG12325 , EcoliWiki:b0136 , ModBase:P37016 , OU-Microarray:b0136 , PortEco:yadK , Protein Model Portal:P37016 , RefSeq:NP_414678 , RegulonDB:EG12325 , String:511145.b0136 , UniProt:P37016

Relationship Links: InterPro:IN-FAMILY:IPR000259 , InterPro:IN-FAMILY:IPR008966 , Pfam:IN-FAMILY:PF00419

GO Terms:

Biological Process: GO:0043709 - cell adhesion involved in single-species biofilm formation Inferred from experiment [Korea10]
GO:0007155 - cell adhesion Inferred by computational analysis [GOA01]
Cellular Component: GO:0009289 - pilus Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA01, Korea10]

MultiFun Terms: cell structure pilus

Essentiality data for yadK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Curated 04-Oct-2006 by Keseler I , SRI International
Last-Curated ? 14-Apr-2010 by Mackie A , Macquarie University


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b0136 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12325; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Korea10: Korea CG, Badouraly R, Prevost MC, Ghigo JM, Beloin C (2010). "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities." Environ Microbiol 12(7):1957-77. PMID: 20345943

Korea11: Korea CG, Ghigo JM, Beloin C (2011). "The sweet connection: Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli fimbriae form a versatile arsenal of sugar-binding lectins potentially involved in surface-colonisation and tissue tropism." Bioessays 33(4);300-11. PMID: 21344461

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.