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Escherichia coli K-12 substr. MG1655 Polypeptide: anti-sigma factor



Gene: rseA Accession Numbers: EG12341 (EcoCyc), b2572, ECK2570

Synonyms: yfiJ, mclA

Regulation Summary Diagram: ?

Summary:
RseA is an anti-sigma factor that inhibits σE [Missiakas97]. RseA is the major regulator of σE [Ades99]. RseA associates with the region of σE that interacts with DNA and may interfere with promoter binding [Tam02]. Structural studies indicate that RseA interferes with binding of σE to core polymerase [Campbell03a]. RseA binding to σE may be regulated by phosphorylation [Klein03].

Upon extracytoplasmic stress, misfolded/unfolded porins interact with the DegS PDZ domain, releasing an inhibitory interaction and activating DegS protease [Walsh03]. DegS and EcfE then sequentially catalyze proteolytic cleavage of RseA, thereby relieving repression of σE and activating the σE-mediated stress response [Kanehara02, Alba02].

The σE system is also involved in the response to hyperosmotic shock [Bianchi99] and in release of nutrients from dead cells during long periods of stationary phase [Nitta00].

A crystal structure of σE in complex with the RseA amino-terminal, cytoplasmic domain is presented at 2 Å resolution [Campbell03a].

RseA resides in the inner membrane. The cytoplasmic amino-terminal domain has the anti-sigma activity [Missiakas97, De97]. Residue Y38 is phosphorylated [Klein03].

Mutations cause increased σE activity [Missiakas97, De97]. Overproduction causes decreased σE activity [Missiakas97].

RseA and σE physically interact [Missiakas97, De97]. RseA also interacts with the periplasmic RseB protein [Missiakas97, De97], which acts to stimulate binding of RseA to σE [Collinet00].

E. coli and mammalian cells exhibit analogous responses to unfolded protein stress [Alba02].

RseA: "regulator of sigma E" [De97]

Citations: [Alba01, Ades03]

Gene Citations: [Raina95, Rhodius05]

Locations: inner membrane

Map Position: [2,706,776 <- 2,707,426] (58.34 centisomes)
Length: 651 bp / 216 aa

Molecular Weight of Polypeptide: 24.321 kD (from nucleotide sequence), 27.7 kD (experimental)

Unification Links: ASAP:ABE-0008462 , CGSC:37241 , DIP:DIP-39581N , DisProt:DP00552 , EchoBASE:EB2245 , EcoGene:EG12341 , EcoliWiki:b2572 , ModBase:P0AFX7 , OU-Microarray:b2572 , PortEco:rseA , PR:PRO_000023877 , Protein Model Portal:P0AFX7 , RefSeq:NP_417067 , RegulonDB:EG12341 , SMR:P0AFX7 , String:511145.b2572 , Swiss-Model:P0AFX7 , UniProt:P0AFX7

Relationship Links: InterPro:IN-FAMILY:IPR005572 , InterPro:IN-FAMILY:IPR005573 , InterPro:IN-FAMILY:IPR026279 , PDB:Structure:1OR7 , PDB:Structure:3M4W , Pfam:IN-FAMILY:PF03872 , Pfam:IN-FAMILY:PF03873

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006950 - response to stress Inferred from experiment Inferred by computational analysis [GOA01a, Ades99]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Ahuja09, Sohn07]
GO:0016989 - sigma factor antagonist activity Inferred from experiment Inferred by computational analysis [GOA01a, Missiakas97]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell processes adaptations other (mechanical, nutritional, oxidative stress)
cell processes adaptations temperature extremes
information transfer RNA related Transcription related
regulation type of regulation transcriptional level sigma factors, anti-sigmafactors

Essentiality data for rseA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes et al., 2003, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba et al., 2006, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba et al., 2006, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 101 -> 118
[UniProt, 2010]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Amino-Acid-Site 109, 108
[UniProt10]
UniProt: Cleavage; by rseP; Sequence Annotation Type: site;
Protein-Segment 162 -> 169
[UniProt09]
UniProt: Poly-Gln; Sequence Annotation Type: compositionally biased region;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b2572 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12341; confirmed by SwissProt match.


References

Ades03: Ades SE, Grigorova IL, Gross CA (2003). "Regulation of the alternative sigma factor sigma(E) during initiation, adaptation, and shutoff of the extracytoplasmic heat shock response in Escherichia coli." J Bacteriol 185(8);2512-9. PMID: 12670975

Ades99: Ades SE, Connolly LE, Alba BM, Gross CA (1999). "The Escherichia coli sigma(E)-dependent extracytoplasmic stress response is controlled by the regulated proteolysis of an anti-sigma factor." Genes Dev 13(18);2449-61. PMID: 10500101

Ahuja09: Ahuja N, Korkin D, Chaba R, Cezairliyan BO, Sauer RT, Kim KK, Gross CA (2009). "Analyzing the interaction of RseA and RseB, the two negative regulators of the sigmaE envelope stress response, using a combined bioinformatic and experimental strategy." J Biol Chem 284(8);5403-13. PMID: 19103591

Alba01: Alba BM, Zhong HJ, Pelayo JC, Gross CA (2001). "degS (hhoB) is an essential Escherichia coli gene whose indispensable function is to provide sigma (E) activity." Mol Microbiol 40(6);1323-33. PMID: 11442831

Alba02: Alba BM, Leeds JA, Onufryk C, Lu CZ, Gross CA (2002). "DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma(E)-dependent extracytoplasmic stress response." Genes Dev 16(16);2156-68. PMID: 12183369

Baba et al., 2006: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bianchi99: Bianchi AA, Baneyx F (1999). "Hyperosmotic shock induces the sigma32 and sigmaE stress regulons of Escherichia coli." Mol Microbiol 34(5);1029-38. PMID: 10594827

Campbell03a: Campbell EA, Tupy JL, Gruber TM, Wang S, Sharp MM, Gross CA, Darst SA (2003). "Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA." Mol Cell 11(4);1067-78. PMID: 12718891

Collinet00: Collinet B, Yuzawa H, Chen T, Herrera C, Missiakas D (2000). "RseB binding to the periplasmic domain of RseA modulates the RseA:sigmaE interaction in the cytoplasm and the availability of sigmaE.RNA polymerase." J Biol Chem 275(43);33898-904. PMID: 11777003

De97: De Las Penas A, Connolly L, Gross CA (1997). "The sigmaE-mediated response to extracytoplasmic stress in Escherichia coli is transduced by RseA and RseB, two negative regulators of sigmaE." Mol Microbiol 24(2);373-85. PMID: 9159523

Gerdes et al., 2003: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kanehara02: Kanehara K, Ito K, Akiyama Y (2002). "YaeL (EcfE) activates the sigma(E) pathway of stress response through a site-2 cleavage of anti-sigma(E), RseA." Genes Dev 16(16);2147-55. PMID: 12183368

Klein03: Klein G, Dartigalongue C, Raina S (2003). "Phosphorylation-mediated regulation of heat shock response in Escherichia coli." Mol Microbiol 48(1);269-85. PMID: 12657060

Missiakas97: Missiakas D, Mayer MP, Lemaire M, Georgopoulos C, Raina S (1997). "Modulation of the Escherichia coli sigmaE (RpoE) heat-shock transcription-factor activity by the RseA, RseB and RseC proteins." Mol Microbiol 24(2);355-71. PMID: 9159522

Nitta00: Nitta T, Nagamitsu H, Murata M, Izu H, Yamada M (2000). "Function of the sigma(E) regulon in dead-cell lysis in stationary-phase Escherichia coli." J Bacteriol 182(18);5231-7. PMID: 10960110

Raina95: Raina S, Missiakas D, Georgopoulos C (1995). "The rpoE gene encoding the sigma E (sigma 24) heat shock sigma factor of Escherichia coli." EMBO J 14(5);1043-55. PMID: 7889935

Rhodius05: Rhodius VA, Suh WC, Nonaka G, West J, Gross CA (2005). "Conserved and variable functions of the sigmaE stress response in related genomes." PLoS Biol 4(1);e2. PMID: 16336047

Sohn07: Sohn J, Grant RA, Sauer RT (2007). "Allosteric activation of DegS, a stress sensor PDZ protease." Cell 131(3);572-83. PMID: 17981123

Tam02: Tam C, Collinet B, Lau G, Raina S, Missiakas D (2002). "Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor." J Biol Chem 277(30);27282-7. PMID: 12016219

UniProt, 2010: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Walsh03: Walsh NP, Alba BM, Bose B, Gross CA, Sauer RT (2003). "OMP peptide signals initiate the envelope-stress response by activating DegS protease via relief of inhibition mediated by its PDZ domain." Cell 113(1);61-71. PMID: 12679035

Other References Related to Gene Regulation

BuryMone09: Bury-Mone S, Nomane Y, Reymond N, Barbet R, Jacquet E, Imbeaud S, Jacq A, Bouloc P (2009). "Global analysis of extracytoplasmic stress signaling in Escherichia coli." PLoS Genet 5(9);e1000651. PMID: 19763168

De02: De Wulf P, McGuire AM, Liu X, Lin EC (2002). "Genome-wide profiling of promoter recognition by the two-component response regulator CpxR-P in Escherichia coli." J Biol Chem 277(29);26652-61. PMID: 11953442

Rouviere95: Rouviere PE, De Las Penas A, Mecsas J, Lu CZ, Rudd KE, Gross CA (1995). "rpoE, the gene encoding the second heat-shock sigma factor, sigma E, in Escherichia coli." EMBO J 14(5);1032-42. PMID: 7889934

Thompson07: Thompson KM, Rhodius VA, Gottesman S (2007). "{sigma}E Regulates and Is Regulated by a Small RNA in Escherichia coli." J Bacteriol 189(11);4243-56. PMID: 17416652

Yamamoto05b: Yamamoto K, Ishihama A (2005). "Transcriptional response of Escherichia coli to external zinc." J Bacteriol 187(18);6333-40. PMID: 16159766


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, BIOCYC14B.