Escherichia coli K-12 substr. MG1655 Polypeptide: e14 prophage; predicted integrase

Gene: intE Accession Numbers: EG12370 (EcoCyc), b1140, ECK1126

Synonyms: int(Lambda), ycfI

Regulation Summary Diagram: ?

Regulation summary diagram for intE

No information about this protein was found by a literature search conducted on February 22, 2006.

Locations: cytosol

Map Position: [1,198,902 <- 1,200,029] (25.84 centisomes, 93°)
Length: 1128 bp / 375 aa

Molecular Weight of Polypeptide: 42.799 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003836 , DIP:DIP-10037N , EchoBASE:EB2273 , EcoGene:EG12370 , EcoliWiki:b1140 , Mint:MINT-1262950 , ModBase:P75969 , OU-Microarray:b1140 , PortEco:intE , PR:PRO_000023027 , Pride:P75969 , Protein Model Portal:P75969 , RefSeq:NP_415658 , RegulonDB:EG12370 , SMR:P75969 , String:511145.b1140 , UniProt:P75969

Relationship Links: InterPro:IN-FAMILY:IPR002104 , InterPro:IN-FAMILY:IPR011010 , InterPro:IN-FAMILY:IPR013087 , InterPro:IN-FAMILY:IPR013762 , InterPro:IN-FAMILY:IPR015094 , InterPro:IN-FAMILY:IPR016177 , InterPro:IN-FAMILY:IPR023109 , Pfam:IN-FAMILY:PF00589 , Pfam:IN-FAMILY:PF09003

GO Terms:

Biological Process: GO:0006310 - DNA recombination Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0015074 - DNA integration Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0046718 - viral entry into host cell Inferred by computational analysis [UniProtGOA11a]
GO:0075713 - establishment of integrated proviral latency Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0046982 - protein heterodimerization activity Inferred from experiment [Carr01]
GO:0003676 - nucleic acid binding Inferred by computational analysis [GOA01a]
GO:0003677 - DNA binding Inferred by computational analysis [GOA01a]
GO:0008907 - integrase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: extrachromosomal prophage genes and phage related functions

Essentiality data for intE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 22-Feb-2006 by Shearer A , SRI International

Sequence Features

Protein sequence of e14 prophage; predicted integrase with features indicated

Feature Class Location Citations Comment
Active-Site 340
UniProt: O-(3'-phospho-DNA)-tyrosine intermediate; Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


1/26/1998 (pkarp) Merged genes G6584/b1140 and EG12370/intE


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Carr01: Carr DW, Fujita A, Stentz CL, Liberty GA, Olson GE, Narumiya S (2001). "Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA." J Biol Chem 276(20);17332-8. PMID: 11278869

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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