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Escherichia coli K-12 substr. MG1655 Polypeptide: QseC sensory histidine kinase



Gene: qseC Accession Numbers: EG12658 (EcoCyc), b3026, ECK3017

Synonyms: ygiY

Regulation Summary Diagram: ?

Alternative forms of QseC sensory histidine kinase: QseC sensory histidine kinase - phosphorylated (summary available)

Summary:
QseC is the sensor kinase component of the QseB-QseC two-component system, which is one component of the quorum-sensing regulatory cascade involved in the regulation of flagella and motility genes. QseBC modulates transcription of flhDC, the master regulator for flagella and motility genes [Sperandio02].

A transposon insertion mutant in qseC is more susceptible to pyrazinoic acid [Schaller02]; qseBC mutants show hypersensitivity to several toxic cations [Zhou03].

qseC: "quorum-sensing E. coli regulator C" [Sperandio02]

A site for RbsR in negatively controlling the qseC gene was identified based on genomic Selex analysis with the absolute center positions in the genome at position 3168522.5 and with the palindromic octanucleotide sequence TTtAcccAACGTcTtaGTcT [Shimada13a].

Gene Citations: [Clarke05]

Locations: inner membrane

Map Position: [3,168,506 -> 3,169,855] (68.29 centisomes)
Length: 1350 bp / 449 aa

Molecular Weight of Polypeptide: 50.282 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009938 , EchoBASE:EB2845 , EcoGene:EG12658 , EcoliWiki:b3026 , ModBase:P40719 , OU-Microarray:b3026 , PortEco:qseC , PR:PRO_000023667 , Pride:P40719 , Protein Model Portal:P40719 , RefSeq:NP_417498 , RegulonDB:EG12658 , SMR:P40719 , String:511145.b3026 , UniProt:P40719

Relationship Links: InterPro:IN-FAMILY:IPR003594 , InterPro:IN-FAMILY:IPR003661 , InterPro:IN-FAMILY:IPR004358 , InterPro:IN-FAMILY:IPR005467 , InterPro:IN-FAMILY:IPR009082 , PDB:Structure:2KSE , PDB:Structure:3JZ3 , Pfam:IN-FAMILY:PF00512 , Pfam:IN-FAMILY:PF02518 , Prints:IN-FAMILY:PR00344 , Prosite:IN-FAMILY:PS50109 , Smart:IN-FAMILY:SM00387 , Smart:IN-FAMILY:SM00388

In Paralogous Gene Group: 122 (29 members)

Reactions known to consume the compound:

QseBC Two-Component Signal Transduction System, quorum sensing related :
QseC[inner membrane] + ATP → QseC sensory histidine kinase - phosphorylated[inner membrane] + ADP

Reactions known to produce the compound:

QseBC Two-Component Signal Transduction System, quorum sensing related :
QseB + QseC sensory histidine kinase - phosphorylated[inner membrane] → QseB-Pasp51 + QseC[inner membrane]

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred by computational analysis [UniProtGOA11a, Sperandio02]
GO:0007165 - signal transduction Inferred by computational analysis [GOA01a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0018106 - peptidyl-histidine phosphorylation Inferred by computational analysis [GOA01]
GO:0023014 - signal transduction by phosphorylation Inferred by computational analysis [GOA01a]
Molecular Function: GO:0000155 - phosphorelay sensor kinase activity Inferred from experiment Inferred by computational analysis [GOA01a, Sperandio02]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0004673 - protein histidine kinase activity Inferred by computational analysis [GOA01]
GO:0004871 - signal transducer activity Inferred by computational analysis [GOA01a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016772 - transferase activity, transferring phosphorus-containing groups Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05, Sperandio02]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, Sperandio02]

MultiFun Terms: information transfer protein related posttranslational modification
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for qseC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Curated 28-Dec-2005 by Keseler I , SRI International
Last-Curated ? 26-Jan-2011 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment State
Transmembrane-Region 13 -> 33
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
 
Transmembrane-Region 157 -> 177
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
 
Conserved-Region 243 -> 449
[UniProt09]
UniProt: Histidine kinase;
 
Phosphorylation-Modification 246
[UniProt13]
UniProt: Phosphohistidine; by autocatalysis; Non-Experimental Qualifier: by similarity.
Unmodified


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
1/26/1998 (pkarp) Merged genes G7576/b3026 and EG12658/ygiO


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Clarke05: Clarke MB, Sperandio V (2005). "Transcriptional autoregulation by quorum sensing Escherichia coli regulators B and C (QseBC) in enterohaemorrhagic E. coli (EHEC)." Mol Microbiol 58(2);441-55. PMID: 16194231

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Schaller02: Schaller A, Guo M, Gisanrin O, Zhang Y (2002). "Escherichia coli genes involved in resistance to pyrazinoic acid, the active component of the tuberculosis drug pyrazinamide." FEMS Microbiol Lett 211(2);265-70. PMID: 12076823

Shimada13a: Shimada T, Kori A, Ishihama A (2013). "Involvement of the ribose operon repressor RbsR in regulation of purine nucleotide synthesis in Escherichia coli." FEMS Microbiol Lett 344(2);159-65. PMID: 23651393

Sperandio02: Sperandio V, Torres AG, Kaper JB (2002). "Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli." Mol Microbiol 43(3);809-21. PMID: 11929534

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhou03: Zhou L, Lei XH, Bochner BR, Wanner BL (2003). "Phenotype microarray analysis of Escherichia coli K-12 mutants with deletions of all two-component systems." J Bacteriol 185(16);4956-72. PMID: 12897016

Other References Related to Gene Regulation

Clarke05a: Clarke MB, Sperandio V (2005). "Transcriptional regulation of flhDC by QseBC and sigma (FliA) in enterohaemorrhagic Escherichia coli." Mol Microbiol 57(6);1734-49. PMID: 16135237

Guckes13: Guckes KR, Kostakioti M, Breland EJ, Gu AP, Shaffer CL, Martinez CR, Hultgren SJ, Hadjifrangiskou M (2013). "Strong cross-system interactions drive the activation of the QseB response regulator in the absence of its cognate sensor." Proc Natl Acad Sci U S A 110(41);16592-7. PMID: 24062463


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, BIOCYC14B.