Escherichia coli K-12 substr. MG1655 Transporter: hexuronate transporter

Gene: exuT Accession Numbers: EG12738 (EcoCyc), b3093, ECK3084

Regulation Summary Diagram: ?

Regulation summary diagram for exuT

ExuT is a transporter for aldohexuronates such as D-galacturonate and D-glucuronate. Mutations affecting the transport of hexuronates have been shown to map to the exuT gene [MataGilsinger83a]. ExuT is a member of the major facilitator superfamily (MFS) of transporters [Pao98] and probably functions as an aldohexuronate/proton symporter. The exuT gene constitutes a hexuronate-inducible single gene operon, whose expression is controlled by the ExuR regulator [Portalier80]. Imported hexuronates are initially metabolised to 2-keto-3-deoxy-D-gluconate.

Gene Citations: [MataGilsinger83, Nemoz76, MataGilsinger83b, HugouvieuxCotte82]

Locations: inner membrane

Map Position: [3,243,246 -> 3,244,544] (69.9 centisomes, 252°)
Length: 1299 bp / 432 aa

Molecular Weight of Polypeptide: 46.892 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010171 , CGSC:800 , EchoBASE:EB2594 , EcoGene:EG12738 , EcoliWiki:b3093 , ModBase:P0AA78 , OU-Microarray:b3093 , PortEco:exuT , PR:PRO_000022558 , Pride:P0AA78 , Protein Model Portal:P0AA78 , RefSeq:NP_417564 , RegulonDB:EG12738 , String:511145.b3093 , UniProt:P0AA78

Relationship Links: InterPro:IN-FAMILY:IPR004744 , InterPro:IN-FAMILY:IPR011701 , InterPro:IN-FAMILY:IPR020846 , Pfam:IN-FAMILY:PF07690 , Prosite:IN-FAMILY:PS50850

In Paralogous Gene Group: 17 (37 members) , 490 (2 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for exuT

GO Terms:

Biological Process: GO:0015736 - hexuronate transport Inferred from experiment [Nemoz76]
GO:0015778 - hexuronide transport Inferred from experiment [Nemoz76]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0055085 - transmembrane transport Inferred by computational analysis [Gaudet10, GOA01a]
Molecular Function: GO:0015134 - hexuronate transmembrane transporter activity Inferred from experiment [Nemoz76]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Pao98]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, Gaudet10, GOA01a]

MultiFun Terms: cell structure membrane
metabolism carbon utilization carbon compounds
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for exuT knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: D-galacturonate transporter (hexuronate transporter)

Synonyms: Transport of galacturonate

Transport reaction diagram for D-galacturonate transporter

Enzymatic reaction of: D-glucuronate transporter (hexuronate transporter)

Synonyms: Transport of glucuronate

Transport reaction diagram for D-glucuronate transporter

Sequence Features

Protein sequence of hexuronate transporter with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 31
Chain 32 -> 432
UniProt: Hexuronate transporter.
Transmembrane-Region 49 -> 69
UniProt: Helical.
Transmembrane-Region 76 -> 96
UniProt: Helical.
Transmembrane-Region 100 -> 120
UniProt: Helical.
Transmembrane-Region 139 -> 159
UniProt: Helical.
Transmembrane-Region 165 -> 185
UniProt: Helical.
Transmembrane-Region 237 -> 257
UniProt: Helical.
Transmembrane-Region 265 -> 285
UniProt: Helical.
Transmembrane-Region 304 -> 323
UniProt: Helical.
Transmembrane-Region 328 -> 350
UniProt: Helical.
Transmembrane-Region 370 -> 390
UniProt: Helical.
Transmembrane-Region 392 -> 412
UniProt: Helical.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


Ingrid Keseler on Tue Apr 29, 2014:
Gene start position changed based on sequence analysis by Eric Neeno-Eckwall.
10/20/97 Gene b3093 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12738; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

HugouvieuxCotte82: Hugouvieux-Cotte-Pattat N, Robert-Baudouy J (1982). "Determination of the transcription direction of the exuT gene in Escherichia coli K-12: divergent transcription of the exuT-uxaCA operons." J Bacteriol 151(1);480-4. PMID: 6282811

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

MataGilsinger83: Mata-Gilsinger M, Ritzenthaler P, Blanco C (1983). "Characterization of the operator sites of the exu regulon in Escherichia coli K-12 by operator-constitutive mutations and repressor titration." Genetics 1983;105(4);829-42. PMID: 6357945

MataGilsinger83a: Mata-Gilsinger M, Ritzenthaler P (1983). "Physical mapping of mutations in the structural gene encoding for the Escherichia coli aldohexuronate transport system." Mol Gen Genet 1983;189(2);355-7. PMID: 6343797

MataGilsinger83b: Mata-Gilsinger M, Ritzenthaler P (1983). "Physical mapping of the exuT and uxaC operators by use of exu plasmids and generation of deletion mutants in vitro." J Bacteriol 155(3);973-82. PMID: 6309752

Nemoz76: Nemoz G, Robert-Baudouy J, Stoeber F (1976). "Physiological and genetic regulation of the aldohexuronate transport system in Escherichia coli." J Bacteriol 1976;127(2);706-18. PMID: 783117

Pao98: Pao SS, Paulsen IT, Saier MH (1998). "Major facilitator superfamily." Microbiol Mol Biol Rev 1998;62(1);1-34. PMID: 9529885

Portalier80: Portalier R, Robert-Baudouy J, Stoeber F (1980). "Regulation of Escherichia coli K-12 hexuronate system genes: exu regulon." J Bacteriol 143(3);1095-107. PMID: 6997263

UniProt14: UniProt Consortium (2014). "UniProt version 2014-08 released on 2014-08-01 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

MedinaRivera11: Medina-Rivera A, Abreu-Goodger C, Thomas-Chollier M, Salgado H, Collado-Vides J, van Helden J (2011). "Theoretical and empirical quality assessment of transcription factor-binding motifs." Nucleic Acids Res 39(3);808-24. PMID: 20923783

Rodionov00: Rodionov DA, Mironov AA, Rakhmaninova AB, Gelfand MS (2000). "Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria." Mol Microbiol 38(4);673-83. PMID: 11115104

Yamamoto08: Yamamoto K, Matsumoto F, Oshima T, Fujita N, Ogasawara N, Ishihama A (2008). "Anaerobic regulation of citrate fermentation by CitAB in Escherichia coli." Biosci Biotechnol Biochem 72(11);3011-4. PMID: 18997424

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed Oct 7, 2015, biocyc12.