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Escherichia coli K-12 substr. MG1655 Polypeptide: ferric enterobactin ABC transporter - ATP binding subunit



Gene: fepC Accession Numbers: EG10295 (EcoCyc), b0588, ECK0581

Regulation Summary Diagram: ?

Regulation summary diagram for fepC

Component of:
ferric enterobactin ABC transporter (summary available)
ferric enterobactin transport system (summary available)

Summary:
Sequence analysis suggests that fepC encodes the ATP-binding subunit of a ferrric enterobactin ABC transporter complex [Shea91]. Transposon mutation of this gene indicates it is essential for ferric enterobactin transport [Pierce86]

Locations: inner membrane, cytosol

Map Position: [618,607 <- 619,422] (13.33 centisomes, 48°)
Length: 816 bp / 271 aa

Molecular Weight of Polypeptide: 29.784 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002025 , CGSC:4987 , EchoBASE:EB0291 , EcoGene:EG10295 , EcoliWiki:b0588 , ModBase:P23878 , OU-Microarray:b0588 , PortEco:fepC , PR:PRO_000022602 , Pride:P23878 , Protein Model Portal:P23878 , RefSeq:NP_415120 , RegulonDB:EG10295 , SMR:P23878 , String:511145.b0588 , UniProt:P23878

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for fepC

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11a]
GO:0034755 - iron ion transmembrane transport Inferred by computational analysis [Shea91]
GO:0055072 - iron ion homeostasis Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0015623 - iron-chelate-transporting ATPase activity Inferred by computational analysis [Shea91]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a, Shea91]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Ozenberger87, Pierce86]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Author statement [Chenault92]

MultiFun Terms: cell processes adaptations Fe aquisition
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for fepC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: ferric enterobactin ABC transporter

Subunit composition of ferric enterobactin ABC transporter = [FepC]2[FepD][FepG][FepB]
         ferric enterobactin ABC transporter - ATP binding subunit = FepC (summary available)
         ferric enterobactin ABC transporter - membrane subunit = FepD (summary available)
         ferric enterobactin ABC transporter - membrane subunit = FepG (summary available)
         ferric enterobactin ABC transporter - periplasmic binding protein = FepB (extended summary available)

Component of: ferric enterobactin transport system (summary available)

Summary:
FepBCDG are components of a ferric enterobactin transport complex that is a member of the ATP-binding cassette (ABC) family of transporters [Ames90, Higgins90]. E. coli K-12 and several other species of Enterobacteriaceae secrete the catecholate siderophore enterobactin. The enterobactin siderophore is a small organic molecule with a high affinity for Fe 3+. FepD and FepG are the integral membrane subunits of the complex [Shea91], FepC is the ATP-binding subunit [Shea91] and FepB is the periplasmic binding protein [Pierce86]. fepDGC form an operon in E. coli K-12 [Chenault91] which is located within a larger cluster of genes reponsible for enterobactin synthesis and ferric entrerobactin transport.

Together with the TonB-dependent outer-membrane transporter, FepA [Shea91], the FepBCDG complex forms a system responsible for the import of ferric enterobactin across the cell envelope

Credits:
Last-Curated ? 14-Oct-2014 by Mackie A , Macquarie University


Enzymatic reaction of: transport of ferric enterobactin (ferric enterobactin ABC transporter)

EC Number: 3.6.3.34

Transport reaction diagram for transport of ferric enterobactin

Alternative Products for ferric enterobactin complex: ferric (2,3-dihydroxybenzoylserine)3 [Hantke90 ]


Subunit of: ferric enterobactin transport system

Subunit composition of ferric enterobactin transport system = [(FepC)2(FepD)(FepG)(FepB)][([TonB][ExbB][ExbD])(FepA)]
         ferric enterobactin ABC transporter = (FepC)2(FepD)(FepG)(FepB) (summary available)
                 ferric enterobactin ABC transporter - ATP binding subunit = FepC (summary available)
                 ferric enterobactin ABC transporter - membrane subunit = FepD (summary available)
                 ferric enterobactin ABC transporter - membrane subunit = FepG (summary available)
                 ferric enterobactin ABC transporter - periplasmic binding protein = FepB (extended summary available)
         ferric enterobactin outer membrane transport complex = ([TonB][ExbB][ExbD])(FepA)
                 TonB energy transducing system = (TonB)(ExbB)(ExbD) (extended summary available)
                         TonB energy transducing system - TonB subunit = TonB (extended summary available)
                         TonB energy transducing system - ExbB subunit = ExbB (summary available)
                         tonB energy transducing system - ExbD subunit = ExbD (summary available)
                 ferric enterobactin / colicin B / colicin D outer membrane porin FepA = FepA (extended summary available)

Summary:
The ferric enterobactin transport system in E. coli K-12 consists of FepA, a high affinity, TonB dependent outer membrane transporter, and FepBCDG, the ATP dependent, ferric enterobactin inner membrane transport complex.


Enzymatic reaction of: ferric enterobactin transport

Transport reaction diagram for ferric enterobactin transport


Sequence Features

Protein sequence of ferric enterobactin ABC transporter - ATP binding subunit with features indicated

Feature Class Location Citations Comment
Conserved-Region 8 -> 244
[UniProt09]
UniProt: ABC transporter;
Nucleotide-Phosphate-Binding-Region 40 -> 47
[UniProt10]
UniProt: ATP; Non-Experimental Qualifier: potential;
Sequence-Conflict 227
[Shea91, UniProt10a]
UniProt: (in Ref. 1; CAA40709);


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Notes:

History:
10/20/97 Gene b0588 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10295; confirmed by SwissProt match.


References

Ames90: Ames GF, Mimura CS, Shyamala V (1990). "Bacterial periplasmic permeases belong to a family of transport proteins operating from Escherichia coli to human: Traffic ATPases." FEMS Microbiol Rev 6(4);429-46. PMID: 2147378

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Barnard01: Barnard TJ, Watson ME, McIntosh MA (2001). "Mutations in the Escherichia coli receptor FepA reveal residues involved in ligand binding and transport." Mol Microbiol 2001;41(3);527-36. PMID: 11532122

Chenault91: Chenault SS, Earhart CF (1991). "Organization of genes encoding membrane proteins of the Escherichia coli ferrienterobactin permease." Mol Microbiol 1991;5(6);1405-13. PMID: 1787794

Chenault92: Chenault SS, Earhart CF (1992). "Identification of hydrophobic proteins FepD and FepG of the Escherichia coli ferrienterobactin permease." J Gen Microbiol 138(10);2167-71. PMID: 1479347

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hantke90: Hantke K (1990). "Dihydroxybenzoylserine--a siderophore for E. coli." FEMS Microbiol Lett 55(1-2);5-8. PMID: 2139424

Higgins90: Higgins CF, Hyde SC, Mimmack MM, Gileadi U, Gill DR, Gallagher MP (1990). "Binding protein-dependent transport systems." J Bioenerg Biomembr 22(4);571-92. PMID: 2229036

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Ozenberger87: Ozenberger BA, Nahlik MS, McIntosh MA (1987). "Genetic organization of multiple fep genes encoding ferric enterobactin transport functions in Escherichia coli." J Bacteriol 169(8);3638-46. PMID: 2956250

Pierce86: Pierce JR, Earhart CF (1986). "Escherichia coli K-12 envelope proteins specifically required for ferrienterobactin uptake." J Bacteriol 166(3);930-6. PMID: 3011753

Pugsley76: Pugsley AP, Reeves P (1976). "Iron uptake in colicin B-resistant mutants of Escherichia coli K-12." J Bacteriol 126(3);1052-62. PMID: 7543

Shea91: Shea CM, McIntosh MA (1991). "Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other periplasmic binding protein-dependent systems in Escherichia coli." Mol Microbiol 1991;5(6);1415-28. PMID: 1838574

Skare93: Skare JT, Ahmer BM, Seachord CL, Darveau RP, Postle K (1993). "Energy transduction between membranes. TonB, a cytoplasmic membrane protein, can be chemically cross-linked in vivo to the outer membrane receptor FepA." J Biol Chem 268(22);16302-8. PMID: 8344918

Sprencel00: Sprencel C, Cao Z, Qi Z, Scott DC, Montague MA, Ivanoff N, Xu J, Raymond KM, Newton SM, Klebba PE (2000). "Binding of ferric enterobactin by the Escherichia coli periplasmic protein FepB." J Bacteriol 182(19);5359-64. PMID: 10986237

Stephens95: Stephens DL, Choe MD, Earhart CF (1995). "Escherichia coli periplasmic protein FepB binds ferrienterobactin." Microbiology 1995;141 ( Pt 7);1647-54. PMID: 7551033

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Chen07: Chen Z, Lewis KA, Shultzaberger RK, Lyakhov IG, Zheng M, Doan B, Storz G, Schneider TD (2007). "Discovery of Fur binding site clusters in Escherichia coli by information theory models." Nucleic Acids Res 35(20);6762-77. PMID: 17921503

Christoffersen01: Christoffersen CA, Brickman TJ, Hook-Barnard I, McIntosh MA (2001). "Regulatory architecture of the iron-regulated fepD-ybdA bidirectional promoter region in Escherichia coli." J Bacteriol 183(6);2059-70. PMID: 11222606

Lavrrar02: Lavrrar JL, Christoffersen CA, McIntosh MA (2002). "Fur-DNA interactions at the bidirectional fepDGC-entS promoter region in Escherichia coli." J Mol Biol 322(5);983-95. PMID: 12367523


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed May 27, 2015, BIOCYC13A.