Escherichia coli K-12 substr. MG1655 Polypeptide: predicted amidotransferase

Gene: yafJ Accession Numbers: G6107 (EcoCyc), b0223, ECK0224

Regulation Summary Diagram: ?

Regulation summary diagram for yafJ

YafJ has similarity to proteins of Neisseria gonorrhoeae [Barten98] and Azospirillum brasilense Yu62 [Ma97].

Locations: cytosol

Map Position: [244,327 -> 245,094] (5.27 centisomes, 19°)
Length: 768 bp / 255 aa

Molecular Weight of Polypeptide: 28.636 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000755 , EchoBASE:EB2941 , EcoGene:EG13147 , EcoliWiki:b0223 , ModBase:Q47147 , OU-Microarray:b0223 , PortEco:yafJ , Pride:Q47147 , Protein Model Portal:Q47147 , RefSeq:NP_414758 , RegulonDB:G6107 , SMR:Q47147 , String:511145.b0223 , Swiss-Model:Q47147 , UniProt:Q47147

Relationship Links: InterPro:IN-FAMILY:IPR017932 , InterPro:IN-FAMILY:IPR026869 , InterPro:IN-FAMILY:IPR029055 , Panther:IN-FAMILY:PTHR30284 , Pfam:IN-FAMILY:PF13230 , Prosite:IN-FAMILY:PS51278

GO Terms:

Biological Process: GO:0006541 - glutamine metabolic process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

Gene Class: ORFs

Essentiality data for yafJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of predicted amidotransferase with features indicated

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
UniProt: Removed; Non-Experimental Qualifier: by similarity.
Chain 2 -> 255
UniProt: Putative glutamine amidotransferase YafJ.
Active-Site 2
UniProt: Non-Experimental Qualifier: by similarity;
Conserved-Region 2 -> 251
UniProt: Glutamine amidotransferase type-2;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Barten98: Barten R, Meyer TF (1998). "Cloning and characterisation of the Neisseria gonorrhoeae aroB gene." Mol Gen Genet 258(1-2);34-44. PMID: 9613570

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Ma97: Ma L, Li J (1997). "Cloning and sequencing of draTG genes and their downstream region of Azospirillum brasilense Yu62." Chin J Biotechnol 13(3);143-52. PMID: 9429775

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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