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Escherichia coli K-12 substr. MG1655 Polypeptide: conserved protein



Gene: yafK Accession Numbers: G6108 (EcoCyc), b0224, ECK0225

Regulation Summary Diagram: ?

Summary:
YafK of enteroaggregative E. coli is required for development of biofilms and for wild-type transcription of genes for type II aggregative adherence fimbriae components [Sheikh01]. YafK of enteroaggregative E. coli is suggested to be secreted [Sheikh01].

Map Position: [245,065 <- 245,805] (5.28 centisomes)
Length: 741 bp / 246 aa

Molecular Weight of Polypeptide: 28.021 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000757 , EchoBASE:EB2942 , EcoGene:EG13148 , EcoliWiki:b0224 , OU-Microarray:b0224 , PortEco:yafK , Pride:P0AA99 , Protein Model Portal:P0AA99 , RefSeq:NP_414759 , RegulonDB:G6108 , String:511145.b0224 , UniProt:P0AA99

Relationship Links: InterPro:IN-FAMILY:IPR005490 , Pfam:IN-FAMILY:PF03734

GO Terms:

Biological Process: GO:0008360 - regulation of cell shape Inferred by computational analysis [UniProtGOA11a]
GO:0009252 - peptidoglycan biosynthetic process Inferred by computational analysis [UniProtGOA12, UniProtGOA11a]
GO:0071555 - cell wall organization Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016757 - transferase activity, transferring glycosyl groups Inferred by computational analysis [UniProtGOA11a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]

Gene Class: ORFs Conserved-ORFs

Essentiality data for yafK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 19
[UniProt10a]
UniProt: Non-Experimental Qualifier: potential;
Chain 20 -> 246
[UniProt09]
UniProt: Uncharacterized protein yafK;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Sheikh01: Sheikh J, Hicks S, Dall'Agnol M, Phillips AD, Nataro JP (2001). "Roles for Fis and YafK in biofilm formation by enteroaggregative Escherichia coli." Mol Microbiol 41(5);983-97. PMID: 11555281

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc13.