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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted oxidoreductase with FAD/NAD(P)-binding domain and dimerization domain; hypochlorite stress response



Gene: rclA Accession Numbers: G6174 (EcoCyc), b0304, ECK0303

Synonyms: ykgC

Regulation Summary Diagram: ?

Summary:
An rclA deletion mutant is more sensitive to HOCl treatment than wild type, and rclA expression is upregulated under RCS (reactive chlorine species) stress conditions [Parker13].

RclA: "reactive chlorine resistance A" [Parker13]

Locations: cytosol

Map Position: [317,900 <- 319,225] (6.85 centisomes)
Length: 1326 bp / 441 aa

Molecular Weight of Polypeptide: 48.244 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001048 , DIP:DIP-12675N , EchoBASE:EB3350 , EcoGene:EG13580 , EcoliWiki:b0304 , Mint:MINT-1306527 , ModBase:P77212 , OU-Microarray:b0304 , PortEco:ykgC , Pride:P77212 , Protein Model Portal:P77212 , RefSeq:NP_414838 , RegulonDB:G6174 , SMR:P77212 , String:511145.b0304 , UniProt:P77212

Relationship Links: InterPro:IN-FAMILY:IPR001327 , InterPro:IN-FAMILY:IPR004099 , InterPro:IN-FAMILY:IPR012999 , InterPro:IN-FAMILY:IPR013027 , InterPro:IN-FAMILY:IPR016156 , InterPro:IN-FAMILY:IPR023753 , Pfam:IN-FAMILY:PF00070 , Pfam:IN-FAMILY:PF02852 , Pfam:IN-FAMILY:PF07992 , Prints:IN-FAMILY:PR00368 , Prosite:IN-FAMILY:PS00076

In Paralogous Gene Group: 40 (11 members)

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:1901530 - response to hypochlorite Inferred from experiment [Parker13]
GO:0006950 - response to stress Inferred by computational analysis [UniProtGOA11a]
GO:0045454 - cell redox homeostasis Inferred by computational analysis [GOA01a]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016668 - oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor Inferred by computational analysis [GOA01a]
GO:0050660 - flavin adenine dinucleotide binding Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes protection

Essentiality data for rclA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 05-Nov-2013 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 33 -> 43
[UniProt10]
UniProt: FAD; Non-Experimental Qualifier: by similarity;
Disulfide-Bond-Site 48, 43
[UniProt10]
UniProt: Redox-active; Non-Experimental Qualifier: by similarity;
Active-Site 426
[UniProt10]
UniProt: Proton acceptor; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Parker13: Parker BW, Schwessinger EA, Jakob U, Gray MJ (2013). "RclR is a Reactive Chlorine-Specific Transcription Factor in Escherichia coli." J Biol Chem. PMID: 24078635

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc11.