Escherichia coli K-12 substr. MG1655 Polypeptide: CusS sensory histidine kinase

Gene: cusS Accession Numbers: G6318 (EcoCyc), b0570, ECK0562

Synonyms: ybcZ, agrS

Regulation Summary Diagram: ?

Regulation summary diagram for cusS

Alternative forms of CusS sensory histidine kinase: CusS sensory histidine kinase - phosphorylated (summary available)

CusS is the sensory histidine kinase of the the CusSR two-component system which regulates expression of the CusCFBA operon in response to elevated copper and silver concentrations [Munson00]. CusS is believed to respond to the presence of Cu(I) [Outten01a]. Disruption of cusS results in increased sensitivity to copper(I) and silver(I) - resistance is restored when cusS is expressed from a plasmid [Gudipaty12]. Disruption of cusS results in Cu(I) accumulation when E.coli is grown under anaerobic conditions [Gudipaty12]. Transcription from the cusCFBA operon is negligible in a cusS deletion strain grown in the presence of Ag(I) [Gudipaty12]. CusS is the primary activator for Ag(I) activated expression of the cusCFBA operon [Gudipaty12].

Autophosphorylation of the purified carboxyterminal domain of CusS and subsequent transfer of the phosphoryl group to CusR has been demonstrated in vitro [Yamamoto05].

Agr: "Ag(I) resistance" [Silver03].

Review: [Rademacher12].

Gene Citations: [Franke01]

Locations: inner membrane

Map Position: [592,551 <- 593,993] (12.77 centisomes, 46°)
Length: 1443 bp / 480 aa

Molecular Weight of Polypeptide: 53.738 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001946 , DIP:DIP-9349N , EchoBASE:EB3406 , EcoGene:EG13642 , EcoliWiki:b0570 , ModBase:P77485 , OU-Microarray:b0570 , PortEco:cusS , PR:PRO_000022354 , Pride:P77485 , Protein Model Portal:P77485 , RefSeq:NP_415102 , RegulonDB:G6318 , SMR:P77485 , String:511145.b0570 , UniProt:P77485

Relationship Links: InterPro:IN-FAMILY:IPR003594 , InterPro:IN-FAMILY:IPR003660 , InterPro:IN-FAMILY:IPR003661 , InterPro:IN-FAMILY:IPR004358 , InterPro:IN-FAMILY:IPR005467 , InterPro:IN-FAMILY:IPR006290 , InterPro:IN-FAMILY:IPR009082 , Pfam:IN-FAMILY:PF00512 , Pfam:IN-FAMILY:PF00672 , Pfam:IN-FAMILY:PF02518 , Prints:IN-FAMILY:PR00344 , Prosite:IN-FAMILY:PS50109 , Prosite:IN-FAMILY:PS50885 , Smart:IN-FAMILY:SM00304 , Smart:IN-FAMILY:SM00387 , Smart:IN-FAMILY:SM00388

In Paralogous Gene Group: 122 (29 members)

Reactions known to consume the compound:

CusSR Two-Component Signal Transduction System :
CusS + ATP → CusS sensory histidine kinase - phosphorylated + ADP

Reactions known to produce the compound:

CusSR Two-Component Signal Transduction System :
CusS sensory histidine kinase - phosphorylated + CusR → CusS + CusR-Pasp51

Genetic Regulation Schematic: ?

Genetic regulation schematic for cusS

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01a, Yamamoto05]
GO:0016310 - phosphorylation Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01a, Yamamoto05]
GO:0023014 - signal transduction by protein phosphorylation Inferred from experiment Inferred by computational analysis [GOA01a, Yamamoto05]
GO:0071280 - cellular response to copper ion Inferred from experiment [Munson00]
GO:0071292 - cellular response to silver ion Inferred from experiment [Franke01]
GO:0007165 - signal transduction Inferred by computational analysis [GOA01a]
GO:0018106 - peptidyl-histidine phosphorylation Inferred by computational analysis [Gaudet10]
Molecular Function: GO:0000155 - phosphorelay sensor kinase activity Inferred from experiment Inferred by computational analysis [GOA01a, Yamamoto05]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0004673 - protein histidine kinase activity Inferred by computational analysis [GOA01, GOA01a]
GO:0004871 - signal transducer activity Inferred by computational analysis [GOA01a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016772 - transferase activity, transferring phosphorus-containing groups Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005622 - intracellular Inferred by computational analysis Inferred from experiment [Yamamoto05, UniProtGOA11, GOA01a]
GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05, Munson00]
GO:0016020 - membrane Author statement Inferred by computational analysis [UniProtGOA11, GOA01a, Franke01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01a, Munson00]

MultiFun Terms: information transfer protein related posttranslational modification
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for cusS knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 26-Jan-2011 by Mackie A , Macquarie University

Sequence Features

Protein sequence of CusS sensory histidine kinase with features indicated

Feature Class Location Citations Comment State
Transmembrane-Region 16 -> 36
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 187 -> 207
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 208 -> 260
UniProt: HAMP;
Conserved-Region 268 -> 480
UniProt: Histidine kinase;
Phosphorylation-Modification 271
UniProt: Phosphohistidine; by autocatalysis; Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Franke01: Franke S, Grass G, Nies DH (2001). "The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions." Microbiology 2001;147(Pt 4);965-72. PMID: 11283292

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Gudipaty12: Gudipaty SA, Larsen AS, Rensing C, McEvoy MM (2012). "Regulation of Cu(I)/Ag(I) efflux genes in Escherichia coli by the sensor kinase CusS." FEMS Microbiol Lett 330(1);30-7. PMID: 22348296

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Munson00: Munson GP, Lam DL, Outten FW, O'Halloran TV (2000). "Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12." J Bacteriol 182(20);5864-71. PMID: 11004187

Outten01a: Outten FW, Huffman DL, Hale JA, O'Halloran TV (2001). "The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli." J Biol Chem 276(33);30670-7. PMID: 11399769

Rademacher12: Rademacher C, Masepohl B (2012). "Copper-responsive gene regulation in bacteria." Microbiology 158(Pt 10);2451-64. PMID: 22918892

Silver03: Silver S (2003). "Bacterial silver resistance: molecular biology and uses and misuses of silver compounds." FEMS Microbiol Rev 27(2-3);341-53. PMID: 12829274

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865

Other References Related to Gene Regulation

Urano15: Urano H, Umezawa Y, Yamamoto K, Ishihama A, Ogasawara H (2015). "Cooperative regulation of the common target genes between H2O2-sensing YedVW and Cu2+-sensing CusSR in Escherichia coli." Microbiology 161(Pt 4);729-38. PMID: 25568260

Yamamoto05a: Yamamoto K, Ishihama A (2005). "Transcriptional response of Escherichia coli to external copper." Mol Microbiol 56(1);215-27. PMID: 15773991

Yang12a: Yang C, Huang TW, Wen SY, Chang CY, Tsai SF, Wu WF, Chang CH (2012). "Genome-wide PhoB binding and gene expression profiles reveal the hierarchical gene regulatory network of phosphate starvation in Escherichia coli." PLoS One 7(10);e47314. PMID: 23071782

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Mon Oct 5, 2015, BIOCYC14A.