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Escherichia coli K-12 substr. MG1655 Polypeptide: DpiA



Gene: dpiA Accession Numbers: G6346 (EcoCyc), b0620, ECK0613

Synonyms: citB, criR, mpdA, DpiA response regulator

Regulation Summary Diagram: ?

Alternative forms of DpiA: DpiA-Pasp DNA-binding transcriptional dual regulator (extended summary available)

Summary:

Gene Citations: [Miller04]

Locations: cytosol

Map Position: [653,085 -> 653,765] (14.08 centisomes)
Length: 681 bp / 226 aa

Molecular Weight of Polypeptide: 25.453 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002131 , DIP:DIP-47831N , EchoBASE:EB3314 , EcoGene:EG13544 , EcoliWiki:b0620 , Mint:MINT-1286196 , ModBase:P0AEF4 , OU-Microarray:b0620 , PortEco:dpiA , PR:PRO_000022471 , Protein Model Portal:P0AEF4 , RefSeq:NP_415153 , RegulonDB:G6346 , SMR:P0AEF4 , String:511145.b0620 , UniProt:P0AEF4

Relationship Links: InterPro:IN-FAMILY:IPR001789 , InterPro:IN-FAMILY:IPR011006 , InterPro:IN-FAMILY:IPR024187 , InterPro:IN-FAMILY:IPR028141 , Pfam:IN-FAMILY:PF00072 , Pfam:IN-FAMILY:PF12431 , Prosite:IN-FAMILY:PS50110 , Smart:IN-FAMILY:SM00448

In Paralogous Gene Group: 121 (40 members)

Reactions known to consume the compound:

DpiBA Two-Component Signal Transduction System :
DpiB sensory histidine kinase - phosphorylated[inner membrane] + DpiA → DpiB[inner membrane] + DpiA-Pasp57

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Yamamoto09, Yamamoto08]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0009605 - response to external stimulus Inferred by computational analysis [GOA01a]
Molecular Function: GO:0000156 - phosphorelay response regulator activity Inferred from experiment [Yamamoto09, Yamamoto08]
GO:0005515 - protein binding Inferred from experiment [Rajagopala14]
GO:0001071 - nucleic acid binding transcription factor activity Inferred by computational analysis [GOA01a]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA01a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
regulation genetic unit regulated operon
regulation type of regulation transcriptional level activator
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Symmetry: Inverted Repeat

Transcription Units regulated by related protein DpiA-Pasp DNA-binding transcriptional dual regulator (8 total):

Essentiality data for dpiA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Curated 05-Mar-2008 by Gama-Castro S , UNAM
Last-Curated ? 23-Jul-2009 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment State
Conserved-Region 6 -> 122
[UniProt09]
UniProt: Response regulatory;
 
4-aspartylphosphate-Modification 57
[UniProt11a]
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity.
Unmodified
DNA-Binding-Region 180 -> 199
[UniProt10a]
UniProt: H-T-H motif; Non-Experimental Qualifier: by similarity;
 


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
3/2/1998 (pkarp) Merged genes G498/EG13544 and G6346/citB
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Miller04: Miller C, Thomsen LE, Gaggero C, Mosseri R, Ingmer H, Cohen SN (2004). "SOS response induction by beta-lactams and bacterial defense against antibiotic lethality." Science 305(5690);1629-31. PMID: 15308764

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yamamoto08: Yamamoto K, Matsumoto F, Oshima T, Fujita N, Ogasawara N, Ishihama A (2008). "Anaerobic regulation of citrate fermentation by CitAB in Escherichia coli." Biosci Biotechnol Biochem 72(11);3011-4. PMID: 18997424

Yamamoto09: Yamamoto K, Matsumoto F, Minagawa S, Oshima T, Fujita N, Ogasawara N, Ishihama A (2009). "Characterization of CitA-CitB signal transduction activating genes involved in anaerobic citrate catabolism in Escherichia coli." Biosci Biotechnol Biochem 73(2);346-50. PMID: 19202292

Other References Related to Gene Regulation

Ingmer98: Ingmer H, Miller CA, Cohen SN (1998). "Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two-component system regulator." Mol Microbiol 29(1);49-59. PMID: 9701802

Mandin09: Mandin P, Gottesman S (2009). "A genetic approach for finding small RNAs regulators of genes of interest identifies RybC as regulating the DpiA/DpiB two-component system." Mol Microbiol 72(3);551-65. PMID: 19426207

Scheu12: Scheu PD, Witan J, Rauschmeier M, Graf S, Liao YF, Ebert-Jung A, Basche T, Erker W, Unden G (2012). "CitA/CitB two-component system regulating citrate fermentation in Escherichia coli and its relation to the DcuS/DcuR system in vivo." J Bacteriol 194(3);636-45. PMID: 22101843


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 21, 2014, biocyc14.