Escherichia coli K-12 substr. MG1655 Enzyme: phosphoethanolamine transferase

Gene: opgE Accession Numbers: G6418 (EcoCyc), b0815, ECK0804

Synonyms: ybiP

Regulation Summary Diagram: ?

Regulation summary diagram for opgE

Osmoregulated periplasmic glutens (OPGs), formerly called membrane derived oligosaccharides (MDOs), from an E. coli K-12 strain lacking opgE do not contain phosphoethanolamine substitutions.
OPGs from an E. coli ΔopgE ΔopgC ΔopgB strain are not substituted with phosphoethanolamine, succinyl nor phosphoglycerol residues.
OPGs from an E. coli ΔopgB ΔopgC strain contain phosphoethanolamine substitutions but do not contain succinyl nor phosphoglycerol substitutions [BontempsGallo13].

OpgE is predicted to contain 4 transmembrane segments plus a periplasmic domain with sulfuric ester hydrolase and transferase activity [BontempsGallo13].

Mutant strains lacking substituents do not have an observable phenotype [BontempsGallo13].

Locations: inner membrane

Map Position: [850,237 <- 851,820] (18.33 centisomes, 66°)
Length: 1584 bp / 527 aa

Molecular Weight of Polypeptide: 59.706 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002786 , EchoBASE:EB3105 , EcoGene:EG13321 , EcoliWiki:b0815 , ModBase:P75785 , OU-Microarray:b0815 , PortEco:ybiP , Pride:P75785 , Protein Model Portal:P75785 , RefSeq:NP_415336 , RegulonDB:G6418 , String:511145.b0815 , UniProt:P75785

Relationship Links: InterPro:IN-FAMILY:IPR000917 , InterPro:IN-FAMILY:IPR017849 , InterPro:IN-FAMILY:IPR017850 , Pfam:IN-FAMILY:PF00884

In Paralogous Gene Group: 204 (5 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0016740 - transferase activity Inferred from experiment Inferred by computational analysis [UniProtGOA11a, BontempsGallo13]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0008484 - sulfuric ester hydrolase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [BontempsGallo13]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure membrane

Essentiality data for opgE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Enzymatic reaction of: phosphoethanolamine transferase

an E. coli osmoregulated periplasmic glucan (OPG)[periplasmic space] + an L-1-phosphatidylethanolamine[periplasmic space] <=> an E. coli osmoregulated periplasmic glucan with phosphotidylethanolamine substituent[periplasmic space] + a 1,2-diacyl-sn-glycerol[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for an E. coli osmoregulated periplasmic glucan (OPG): arbutin [BontempsGallo13 ]

Sequence Features

Protein sequence of phosphoethanolamine transferase with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 34 -> 54
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 63 -> 83
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 107 -> 127
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 147 -> 167
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BontempsGallo13: Bontemps-Gallo S, Cogez V, Robbe-Masselot C, Quintard K, Dondeyne J, Madec E, Lacroix JM (2013). "Biosynthesis of Osmoregulated Periplasmic Glucans in Escherichia coli: The Phosphoethanolamine Transferase Is Encoded by opgE." Biomed Res Int 2013;371429. PMID: 24228245

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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