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Escherichia coli K-12 substr. MG1655 Polypeptide: protein required for succinyl modification of osmoregulated periplasmic glucans



Gene: opgC Accession Numbers: G6552 (EcoCyc), b1047, ECK1033

Synonyms: ymdD, mdoC

Regulation Summary Diagram: ?

Summary:
OpgC is required for wild-type succinyl modification of osmoregulated periplasmic glucans (OPGs) [Lacroix99] formerly known as membrane derived oligosaccharides (MDOs).

An opgC mutation causes loss of succinyl modification of OPGs in an opgB mutant background, which lacks the wild-type phosphoglycerol modification of OPGs [Lacroix99].

OpgC is predicted to be an integral membrane protein [Lacroix99].

Expression of the Rhodobacter sphaeroides opgIHC gene cluster in an E. coli opgB opgC double mutant partially functionally complements the mutant defect in OPG biosynthesis [Cogez02].

Locations: inner membrane

Map Position: [1,107,007 <- 1,108,164] (23.86 centisomes)
Length: 1158 bp / 385 aa

Molecular Weight of Polypeptide: 44.69 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003553 , EchoBASE:EB3635 , EcoGene:EG13876 , EcoliWiki:b1047 , OU-Microarray:b1047 , PortEco:mdoC , PR:PRO_000023178 , Pride:P75920 , Protein Model Portal:P75920 , RefSeq:NP_415565 , RegulonDB:G6552 , String:511145.b1047 , UniProt:P75920

Relationship Links: InterPro:IN-FAMILY:IPR002656 , InterPro:IN-FAMILY:IPR023723 , Pfam:IN-FAMILY:PF01757

GO Terms:

Biological Process: GO:0009311 - oligosaccharide metabolic process Inferred from experiment [Lacroix99]
GO:0009250 - glucan biosynthetic process Inferred by computational analysis [GOA06]
Molecular Function: GO:0016748 - succinyltransferase activity Inferred from experiment [Lacroix99]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016741 - transferase activity, transferring one-carbon groups Inferred by computational analysis [GOA06, GOA01]
GO:0016746 - transferase activity, transferring acyl groups Inferred by computational analysis [UniProtGOA11]
GO:0016747 - transferase activity, transferring acyl groups other than amino-acyl groups Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06, DiazMejia09, Daley05, Lacroix99]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell processes adaptations osmotic pressure
cell structure membrane
metabolism biosynthesis of macromolecules (cellular constituents) osmoregulated periplasmic glucan

Essentiality data for opgC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 17 -> 37
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 60 -> 80
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 91 -> 111
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 137 -> 157
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 173 -> 193
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 213 -> 233
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 239 -> 259
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 274 -> 294
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 311 -> 331
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 338 -> 358
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Amanda Mackie on Thu Dec 19, 2013:
20/12/2013 - membrane derived oligosaccharides (MDOs) are now called osmoregulated periplasmic glucans (OPGs) and the gene names have been adjusted accordingly
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Cogez02: Cogez V, Gak E, Puskas A, Kaplan S, Bohin JP (2002). "The opgGIH and opgC genes of Rhodobacter sphaeroides form an operon that controls backbone synthesis and succinylation of osmoregulated periplasmic glucans." Eur J Biochem 269(10);2473-84. PMID: 12027885

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lacroix99: Lacroix JM, Lanfroy E, Cogez V, Lequette Y, Bohin A, Bohin JP (1999). "The mdoC gene of Escherichia coli encodes a membrane protein that is required for succinylation of osmoregulated periplasmic glucans." J Bacteriol 181(12);3626-31. PMID: 10368134

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.