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Escherichia coli K-12 substr. MG1655 Enzyme: anhydro-N-acetylmuramic acid kinase



Gene: anmK Accession Numbers: G6880 (EcoCyc), b1640, ECK1636

Synonyms: ydhH

Regulation Summary Diagram: ?

Summary:
AnmK is one of the enzymes responsible for the recycling of murein. Anhydro-N-acetylmuramic acid (anhMurNAc) is produced by the cleavage of muropeptide by NagZ (between the GlcNAc and the anhMurNAc moieties) and AmpD (between the anhMurNAc and peptide moieties), and AnmK catalyzes the hydrolysis of the 1,6-anhydro bond and simultaneous phosphorylation of anhMurNAc to N-acetylmuramate 6-phosphate. An etherase then cleaves the ether bond between the GlcNAc and D-lactate moieties of N-acetylmuramate 6-phosphate [Uehara05].

In an anmK null mutant, anhMurNAc does not accumulate inside the cell, although no other enzyme activity that is able to modify anhMurNAc can be detected. When anhMurNAc can not be phosphorylated, it is lost into the medium instead [Uehara05].

Review: [Park08b]

Map Position: [1,716,517 <- 1,717,626] (37.0 centisomes)
Length: 1110 bp / 369 aa

Molecular Weight of Polypeptide: 39.496 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005488 , DIP:DIP-11731N , EchoBASE:EB3700 , EcoGene:EG13942 , EcoliWiki:b1640 , OU-Microarray:b1640 , PortEco:anmK , PR:PRO_000022102 , Pride:P77570 , Protein Model Portal:P77570 , RefSeq:NP_416157 , RegulonDB:G6880 , SMR:P77570 , String:511145.b1640 , UniProt:P77570

Relationship Links: InterPro:IN-FAMILY:IPR005338 , Pfam:IN-FAMILY:PF03702

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0006040 - amino sugar metabolic process Inferred by computational analysis [GOA06, GOA01a]
GO:0009254 - peptidoglycan turnover Inferred by computational analysis [UniProtGOA12, GOA06, GOA01a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
GO:0097175 - 1,6-anhydro-N-acetyl-beta-muramic acid catabolic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0016301 - kinase activity Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Uehara05]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016773 - phosphotransferase activity, alcohol group as acceptor Inferred by computational analysis [GOA06, GOA01a]

MultiFun Terms: metabolism central intermediary metabolism murein turnover, recycling

Essentiality data for anmK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: anhydro-N-acetylmuramic acid kinase

EC Number: 2.7.1.170

1,6-anhydro-N-acetyl-β-muramate + ATP + H2O <=> N-acetyl-β-muramate 6-phosphate + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: anhydromuropeptides recycling

Cofactors or Prosthetic Groups: Mg2+ [Uehara05]

Inhibitors (Unknown Mechanism): ADP [Uehara05]

Kinetic Parameters:

Substrate
Km (μM)
Citations
1,6-anhydro-N-acetyl-β-muramate
1000.0, 1000.0
[Uehara05]
ATP
1000.0, 1000.0
[Uehara05]

pH(opt): 10 [Uehara05]


Sequence Features

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 12 -> 19
[UniProt10a]
UniProt: ATP; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Park08b: Park JT, Uehara T (2008). "How bacteria consume their own exoskeletons (turnover and recycling of cell wall peptidoglycan)." Microbiol Mol Biol Rev 72(2);211-27, table of contents. PMID: 18535144

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Uehara05: Uehara T, Suefuji K, Valbuena N, Meehan B, Donegan M, Park JT (2005). "Recycling of the anhydro-N-acetylmuramic acid derived from cell wall murein involves a two-step conversion to N-acetylglucosamine-phosphate." J Bacteriol 187(11);3643-9. PMID: 15901686

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC14B.