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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted FAD-linked oxidoreductase



Gene: ydiJ Accession Numbers: G6913 (EcoCyc), b1687, ECK1684

Regulation Summary Diagram: ?

Summary:
YdiJ has similarity to a nonessential protein of Pseudomonas putida [Ramos00].

Map Position: [1,763,653 <- 1,766,709] (38.01 centisomes)
Length: 3057 bp / 1018 aa

Molecular Weight of Polypeptide: 113.25 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005632 , DIP:DIP-11751N , EchoBASE:EB3726 , EcoGene:EG13969 , EcoliWiki:b1687 , ModBase:P77748 , OU-Microarray:b1687 , PortEco:ydiJ , Pride:P77748 , Protein Model Portal:P77748 , RefSeq:NP_416202 , RegulonDB:G6913 , SMR:P77748 , String:511145.b1687 , UniProt:P77748

Relationship Links: InterPro:IN-FAMILY:IPR004113 , InterPro:IN-FAMILY:IPR006094 , InterPro:IN-FAMILY:IPR009051 , InterPro:IN-FAMILY:IPR012285 , InterPro:IN-FAMILY:IPR016164 , InterPro:IN-FAMILY:IPR016166 , InterPro:IN-FAMILY:IPR016167 , InterPro:IN-FAMILY:IPR016169 , InterPro:IN-FAMILY:IPR017896 , InterPro:IN-FAMILY:IPR017900 , Pfam:IN-FAMILY:PF01565 , Pfam:IN-FAMILY:PF02913 , Pfam:IN-FAMILY:PF13183 , Prosite:IN-FAMILY:PS00198 , Prosite:IN-FAMILY:PS51379 , Prosite:IN-FAMILY:PS51387

In Paralogous Gene Group: 331 (2 members)

GO Terms:

Biological Process: GO:0055114 - oxidation-reduction process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0008762 - UDP-N-acetylmuramate dehydrogenase activity Inferred by computational analysis [GOA01a]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [GOA01a]
GO:0016614 - oxidoreductase activity, acting on CH-OH group of donors Inferred by computational analysis [GOA01a]
GO:0050660 - flavin adenine dinucleotide binding Inferred by computational analysis [GOA01a]
GO:0051536 - iron-sulfur cluster binding Inferred by computational analysis [GOA01a]

Gene Class: ORFs

Essentiality data for ydiJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 11-Oct-2006 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 48 -> 281
[UniProt09]
UniProt: FAD-binding PCMH-type;
Conserved-Region 662 -> 695
[UniProt09]
UniProt: 4Fe-4S ferredoxin-type;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Ramos00: Ramos C, Molina L, Molbak L, Ramos JL, Molin S (2000). "A bioluminescent derivative of Pseudomonas putida KT2440 for deliberate release into the environment." FEMS Microbiol Ecol 34(2);91-102. PMID: 11102686

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, biocyc14.