Escherichia coli K-12 substr. MG1655 Polypeptide: conserved protein

Gene: yoaA Accession Numbers: G6992 (EcoCyc), b1808, ECK1806

Regulation Summary Diagram: ?

Regulation summary diagram for yoaA

Mutations in yoaA are synergistic with mutations in radA for sensitivity to the DNA damaging agent azidothymidine [Cooper15].

Locations: cytosol

Map Position: [1,889,349 <- 1,891,259] (40.72 centisomes, 147°)
Length: 1911 bp / 636 aa

Molecular Weight of Polypeptide: 70.378 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006016 , DIP:DIP-12786N , EchoBASE:EB3286 , EcoGene:EG13513 , EcoliWiki:b1808 , ModBase:P76257 , OU-Microarray:b1808 , PortEco:yoaA , Pride:P76257 , Protein Model Portal:P76257 , RefSeq:NP_416322 , RegulonDB:G6992 , SMR:P76257 , String:511145.b1808 , UniProt:P76257

Relationship Links: InterPro:IN-FAMILY:IPR006555 , InterPro:IN-FAMILY:IPR006935 , InterPro:IN-FAMILY:IPR010614 , InterPro:IN-FAMILY:IPR014001 , InterPro:IN-FAMILY:IPR014013 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF04851 , Pfam:IN-FAMILY:PF06733 , Pfam:IN-FAMILY:PF13307 , Prosite:IN-FAMILY:PS51193 , Smart:IN-FAMILY:SM00487 , Smart:IN-FAMILY:SM00491

In Paralogous Gene Group: 202 (2 members)

GO Terms:

Biological Process: GO:0006139 - nucleobase-containing compound metabolic process Inferred by computational analysis [GOA01a]
GO:0032508 - DNA duplex unwinding Inferred by computational analysis [GOA01a]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0003676 - nucleic acid binding Inferred by computational analysis [GOA01a]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0004003 - ATP-dependent DNA helicase activity Inferred by computational analysis [GOA01a]
GO:0004386 - helicase activity Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0008026 - ATP-dependent helicase activity Inferred by computational analysis [GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]


Essentiality data for yoaA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of conserved protein with features indicated

Feature Class Location Citations Comment
Conserved-Region 10 -> 272
UniProt: Helicase ATP-binding;
Nucleotide-Phosphate-Binding-Region 45 -> 52
UniProt: ATP; Non-Experimental Qualifier: potential;
Protein-Segment 193 -> 196
UniProt: Poly-Val; Sequence Annotation Type: compositionally biased region;
Protein-Segment 225 -> 228
UniProt: DEAH box; Sequence Annotation Type: short sequence motif.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Cooper15: Cooper DL, Boyle DC, Lovett ST (2015). "Genetic analysis of Escherichia coli RadA: functional motifs and genetic interactions." Mol Microbiol 95(5);769-79. PMID: 25484163

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc13.