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Escherichia coli K-12 substr. MG1655 Polypeptide: copper homeostasis protein

Gene: cutC Accession Numbers: G7024 (EcoCyc), b1874, ECK1875

Regulation Summary Diagram: ?

A combination of mutations in nlpE and cutC results in increased sensitivity to copper [Gupta95].

Locations: cytosol

Map Position: [1,956,544 <- 1,956,984] (42.17 centisomes)
Length: 441 bp / 146 aa

Molecular Weight of Polypeptide: 15.873 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006252 , EchoBASE:EB2790 , EcoGene:EG12956 , EcoliWiki:b1874 , ModBase:P67826 , OU-Microarray:b1874 , PortEco:cutC , Pride:P67826 , Protein Model Portal:P67826 , RefSeq:YP_025309 , RegulonDB:G7024 , SMR:P67826 , String:511145.b1874 , Swiss-Model:P67826 , UniProt:P67826

Relationship Links: InterPro:IN-FAMILY:IPR005627 , InterPro:IN-FAMILY:IPR023648 , Panther:IN-FAMILY:PTHR12598 , Pfam:IN-FAMILY:PF03932

GO Terms:

Biological Process: GO:0006878 - cellular copper ion homeostasis Inferred from experiment [Gupta95]
GO:0046688 - response to copper ion Inferred from experiment [Gupta95]
GO:0055070 - copper ion homeostasis Inferred from experiment Inferred by computational analysis [GOA01a, Gupta95]
Molecular Function: GO:0005507 - copper ion binding Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes protection detoxification

Essentiality data for cutC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Last-Curated ? 15-May-2007 by Keseler I , SRI International

Sequence Features

Feature Class Location Citations Comment
Sequence-Conflict 18
[Gupta96, UniProt10a]
Alternate sequence: F → H; UniProt: (in Ref. 1; AAA89202);

Gene Local Context (not to scale): ?

Transcription Unit:


Socorro Gama-Castro on Wed Nov 8, 2006:
The start site of the cutC gene was originally assigned solely on the basis of sequence considerations [Blattner97 , Sawers89 ]. However, it was changed because [Gupta95 ] attested that the real start site is actually located 306 bp downstream. The demonstration is based on identification of both a plausible ribosome binding site and one promoter at an appropriate distance from the new start site. The promoter was identified using primer extension analysis [Dartigalongue01 ].
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Blattner97: Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997). "The complete genome sequence of Escherichia coli K-12." Science 277(5331);1453-74. PMID: 9278503

Dartigalongue01: Dartigalongue C, Missiakas D, Raina S (2001). "Characterization of the Escherichia coli sigma E regulon." J Biol Chem 276(24);20866-75. PMID: 11274153

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Gupta95: Gupta SD, Lee BT, Camakaris J, Wu HC (1995). "Identification of cutC and cutF (nlpE) genes involved in copper tolerance in Escherichia coli." J Bacteriol 1995;177(15);4207-15. PMID: 7635807

Gupta96: Gupta S.D., Wu H.C. (1996). "Complete sequence of the E. coli cutC gene." Data submission to EMBL/GenBank/DDBJ databases on 1996-02.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Sawers89: Sawers G, Bock A (1989). "Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression." J Bacteriol 1989;171(5);2485-98. PMID: 2651404

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Tue Jan 27, 2015, BIOCYC13B.