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Escherichia coli K-12 substr. MG1655 Polypeptide: universal stress protein



Gene: uspC Accession Numbers: G7031 (EcoCyc), b1895, ECK1894

Synonyms: yecG

Regulation Summary Diagram: ?

Summary:
UspC, UspD, and UspE play nonredundant and probably sequential roles in a pathway involved in resistance to UV irradiation [Gustavsson02].

Mutation of the uspC, uspD, or uspE gene causes decreased resistance to UV irradiation [Gustavsson02].

Under conditions of salt stress UspC is believed to act as a scaffolding protein for the KdpD/KdpE phosphorylation cascade. UspC interacts with the usp domain of KdpD sensory histidine kinase / protein phosphatase and stable complexes of UspC/KdpD/phosphorylated KdpE/DNA have been observed in vitro. uspC null mutants show down regulation of KdpFABC production under conditions of salt stress [Heermann09, Heermann09a].

The protein has been detected and mapped on a 2-D gel [Gustavsson02].

Regulation has been described [Gustavsson02]. The uspC, uspD, and uspE genes appear to be coregulated; the stresses that act as inducers and the genetics of this regulation is examined [Gustavsson02].

UspC: "universal stress protein" [Gustavsson02].

Citations: [Serres01]

Locations: cytosol

Map Position: [1,977,777 -> 1,978,205] (42.63 centisomes)
Length: 429 bp / 142 aa

Molecular Weight of Polypeptide: 15.633 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006316 , EchoBASE:EB2704 , EcoGene:EG12862 , EcoliWiki:b1895 , ModBase:P46888 , OU-Microarray:b1895 , PortEco:uspC , PR:PRO_000024192 , Protein Model Portal:P46888 , RefSeq:NP_416409 , RegulonDB:G7031 , SMR:P46888 , String:511145.b1895 , UniProt:P46888

Relationship Links: InterPro:IN-FAMILY:IPR006015 , InterPro:IN-FAMILY:IPR006016 , InterPro:IN-FAMILY:IPR014729 , Pfam:IN-FAMILY:PF00582

In Paralogous Gene Group: 532 (3 members)

GO Terms:

Biological Process: GO:0006950 - response to stress Inferred by computational analysis [GOA01]
Molecular Function: GO:0032947 - protein complex scaffold Inferred from experiment [Heermann09, Heermann09a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes adaptations
cell processes protection radiation

Essentiality data for uspC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 23-Sep-2009 by Mackie A , Macquarie University


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Gustavsson02: Gustavsson N, Diez A, Nystrom T (2002). "The universal stress protein paralogues of Escherichia coli are co-ordinately regulated and co-operate in the defence against DNA damage." Mol Microbiol 43(1);107-17. PMID: 11849540

Heermann09: Heermann R, Weber A, Mayer B, Ott M, Hauser E, Gabriel G, Pirch T, Jung K (2009). "The universal stress protein UspC scaffolds the KdpD/KdpE signaling cascade of Escherichia coli under salt stress." J Mol Biol 386(1);134-48. PMID: 19101563

Heermann09a: Heermann R, Lippert ML, Jung K (2009). "Domain swapping reveals that the N-terminal domain of the sensor kinase KdpD in Escherichia coli is important for signaling." BMC Microbiol 9;133. PMID: 19589130

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC13A.