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Escherichia coli K-12 substr. MG1655 Polypeptide: L-cystine ABC transporter - predicted membrane subunit



Gene: yecS Accession Numbers: G7037 (EcoCyc), b1918, ECK1917

Regulation Summary Diagram: ?

Regulation summary diagram for yecS

Component of: L-cystine / L-cysteine ABC transporter (summary available)

Summary:
YecS is an inner membrane protein with three predicted transmembrane domains. The C terminus is located in the cytoplasm [Daley05]. yecS encodes the integral membrane subunit of an L-cystine/L-cysteine ABC transporter complex [Kertesz01, Deutch14].

Locations: inner membrane

Map Position: [1,995,835 <- 1,996,503] (43.02 centisomes, 155°)
Length: 669 bp / 222 aa

Molecular Weight of Polypeptide: 24.801 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006386 , EchoBASE:EB3791 , EcoGene:EG14037 , EcoliWiki:b1918 , OU-Microarray:b1918 , PortEco:yecS , Protein Model Portal:P0AFT2 , RefSeq:NP_416428 , RegulonDB:G7037 , SMR:P0AFT2 , String:511145.b1918 , UniProt:P0AFT2

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR010065 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0015811 - L-cystine transport Inferred from experiment [Deutch14]
GO:0042883 - cysteine transport Inferred from experiment [Deutch14]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0015184 - L-cystine transmembrane transporter activity Inferred from experiment [Deutch14]
GO:0033229 - cysteine transmembrane transporter activity Inferred from experiment [Deutch14]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Kertesz01]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Kertesz01]

MultiFun Terms: cell structure membrane
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for yecS knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 19-Jan-2007 by Keseler I , SRI International


Subunit of: L-cystine / L-cysteine ABC transporter

Subunit composition of L-cystine / L-cysteine ABC transporter = [YecC][YecS][FliY]
         L-cystine ABC transporter - predicted ATP binding subunit = YecC (summary available)
         L-cystine ABC transporter - predicted membrane subunit = YecS (summary available)
         L-cystine ABC transporter - periplasmic binding protein = FliY (extended summary available)

Summary:
fliY, yecS and yecC encode proteins that constitute an L-cystine/L-cysteine transporter complex in E. coli K-12. The complex is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters; FliY is the periplasmic binding protein [Butler93] and sequence analysis suggests that YecC and YecS are the ATP binding subunit and integral membrane subunit respectively [Kertesz01].

E. coli K-12 contains a second cystine transporter encoded by ydjN. An E. coli ΔfliY ΔydjN strain displays minimal L-cystine uptake. Inhibition assays suggest that the FliYYecSC transporter has a greater substrate range than YdjN. L-cysteine may be the preferred substrate for the FliYYecSC transporter. The complex is also able to transport djenkolic acid and the toxic compounds L-selenaproline and L-selenocystine [Deutch14].

Citations: [Berger72, Kull97]

Locations: inner membrane

GO Terms:

Biological Process: GO:0015811 - L-cystine transport Inferred from experiment [Deutch14]
GO:0042883 - cysteine transport Inferred from experiment [Deutch14]
Molecular Function: GO:0015184 - L-cystine transmembrane transporter activity Inferred from experiment [Deutch14]
GO:0033229 - cysteine transmembrane transporter activity Inferred from experiment [Deutch14]
GO:0015426 - polar-amino acid-transporting ATPase activity Inferred by computational analysis [Kertesz01]
Cellular Component: GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Kertesz01]

Credits:
Created 06-Oct-2014 by Mackie A , Macquarie University


Enzymatic reaction of: L-cysteine ABC transporter

Transport reaction diagram for L-cysteine ABC transporter


Enzymatic reaction of: L-cystine ABC transporter (L-cystine / L-cysteine ABC transporter)

Transport reaction diagram for L-cystine ABC transporter

Activators (Unknown Mechanism): D-cysteine [Deutch14] , L-dithiothreitol [Deutch14]

Inhibitors (Unknown Mechanism): DL-selenocystine [Deutch14] , djenkolate [Deutch14] , meso-diaminopimelate [Deutch14] , L-cysteine [Deutch14] , L-cystathionine [Deutch14]

Kinetic Parameters:

Substrate
Km (μM)
Vmax (µmol mg-1 min-1)
Citations
L-cystine
1.25
6.2e-4
[Deutch14]


Sequence Features

Protein sequence of L-cystine ABC transporter - predicted membrane subunit with features indicated

Feature Class Location Citations Comment
Conserved-Region 19 -> 207
[UniProt09]
UniProt: ABC transmembrane type-1;
Transmembrane-Region 23 -> 43
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 65 -> 85
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 183 -> 203
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Berger72: Berger EA, Heppel LA (1972). "A binding protein involved in the transport of cystine and diaminopimelic acid in Escherichia coli." J Biol Chem 247(23);7684-94. PMID: 4564569

Butler93: Butler JD, Levin SW, Facchiano A, Miele L, Mukherjee AB (1993). "Amino acid composition and N-terminal sequence of purified cystine binding protein of Escherichia coli." Life Sci 1993;52(14);1209-15. PMID: 8450713

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

Deutch14: Deutch CE, Spahija I, Wagner CE (2014). "Susceptibility of Escherichia coli to the toxic L-proline analogue L-selenaproline is dependent on two L-cystine transport systems." J Appl Microbiol 117(5);1487-99. PMID: 25139244

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kertesz01: Kertesz MA (2001). "Bacterial transporters for sulfate and organosulfur compounds." Res Microbiol 152(3-4);279-90. PMID: 11421275

Kull97: Kull FJ, Lindblow-Kull C, Shrift A (1997). "The transport systems for selenomethionine/methionine and selenocystine/cystine in Escherichia coli K-12 appear to be cooperative." Membr Biochem 7(4);207-17. PMID: 3077145

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Jul 7, 2015, biocyc11.