Escherichia coli K-12 substr. MG1655 Enzyme: Mlc titration factor

Gene: mtfA Accession Numbers: G7063 (EcoCyc), b1976, ECK1972

Synonyms: yeeI

Regulation Summary Diagram: ?

Regulation summary diagram for mtfA

MtfA interacts with the transcriptional repressor Mlc, thereby modulating the expression of genes which are regulated by Mlc [Becker06]. Binding of MtfA to Mlc prevents interaction of Mlc with the ptsG operator region [Gohler12].

The C-terminal region of MtfA is responsible for interaction with Mlc [Gohler12].

MtfA has aminopeptidase activity. The Mlc-binding and peptidase activities of MtfA are separable, and Mlc does not appear to be a biologically relevant peptidase substrate [Gohler12].

An mtfA insertion mutant shows slow growth on glucose and decreased expression of ptsG, the gene encoding the major glucose transporter in the cell. Expression of ptsG is regulated by several mechanisms, including transcriptional repression by Mlc. Mlc is epistatic to MtfA, and MtfA interacts with Mlc directly in vitro [Becker06].

MtfA: "Mlc titration factor A" [Becker06]

Locations: cytosol

Map Position: [2,041,675 -> 2,042,472] (44.0 centisomes, 158°)
Length: 798 bp / 265 aa

Molecular Weight of Polypeptide: 30.279 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006558 , EchoBASE:EB3159 , EcoGene:EG13377 , EcoliWiki:b1976 , OU-Microarray:b1976 , PortEco:mtfA , PR:PRO_000023301 , Pride:P76346 , Protein Model Portal:P76346 , RefSeq:NP_416484 , RegulonDB:G7063 , SMR:P76346 , String:511145.b1976 , UniProt:P76346

Relationship Links: InterPro:IN-FAMILY:IPR010384 , InterPro:IN-FAMILY:IPR024079 , Pfam:IN-FAMILY:PF06167

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006508 - proteolysis Inferred from experiment Inferred by computational analysis [GOA01a, Gohler12]
GO:0043433 - negative regulation of sequence-specific DNA binding transcription factor activity Inferred from experiment [Gohler12]
Molecular Function: GO:0004177 - aminopeptidase activity Inferred from experiment [Gohler12]
GO:0005515 - protein binding Inferred from experiment [Gohler12, Becker06]
GO:0008134 - transcription factor binding Inferred from experiment [Becker06]
GO:0008237 - metallopeptidase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Becker06]
GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06]

MultiFun Terms: regulation type of regulation posttranscriptional

Essentiality data for mtfA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Last-Curated ? 02-Feb-2012 by Keseler I , SRI International

Enzymatic reaction of: aminopeptidase (Mlc titration factor)

EC Number: 3.4.11.-

a protein + H2O <=> a peptide + a standard α amino acid

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Becker06: Becker AK, Zeppenfeld T, Staab A, Seitz S, Boos W, Morita T, Aiba H, Mahr K, Titgemeyer F, Jahreis K (2006). "YeeI, a Novel Protein Involved in Modulation of the Activity of the Glucose-Phosphotransferase System in Escherichia coli K-12." J Bacteriol 188(15);5439-49. PMID: 16855233

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Gohler12: Gohler AK, Staab A, Gabor E, Homann K, Klang E, Kosfeld A, Muus JE, Wulftange JS, Jahreis K (2012). "Characterization of MtfA, a novel regulatory output signal protein of the glucose-phosphotransferase system in Escherichia coli K-12." J Bacteriol 194(5);1024-35. PMID: 22178967

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc12.