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Escherichia coli K-12 substr. MG1655 Polypeptide: undecaprenyl-phosphate-α-L-Ara4N flippase - ArnF subunit



Gene: arnF Accession Numbers: G7171 (EcoCyc), b2258, ECK2252

Synonyms: yfbJ

Regulation Summary Diagram: ?

Component of: undecaprenyl-phosphate-α-L-Ara4N flippase (extended summary available)

Summary:
ArnF is an integral membrane component of the ArnE/ArnF undecaprenyl-phosphate-α-L-Ara4N flippase.

Locations: inner membrane

Map Position: [2,370,914 -> 2,371,300] (51.1 centisomes)
Length: 387 bp / 128 aa

Molecular Weight of Polypeptide: 14.085 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007466 , DIP:DIP-48225N , EchoBASE:EB3847 , EcoGene:EG14094 , EcoliWiki:b2258 , OU-Microarray:b2258 , PortEco:arnF , Protein Model Portal:P76474 , RefSeq:NP_416761 , RegulonDB:G7171 , String:511145.b2258 , UniProt:P76474

Relationship Links: InterPro:IN-FAMILY:IPR022832 , Pfam:IN-FAMILY:PF00892

In Paralogous Gene Group: 398 (2 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Yan07]
GO:0010041 - response to iron(III) ion Inferred from experiment [Chamnongpol02, Hagiwara04]
GO:0006629 - lipid metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0009103 - lipopolysaccharide biosynthetic process Inferred by computational analysis [UniProtGOA12, UniProtGOA11a]
GO:0009245 - lipid A biosynthetic process Inferred by computational analysis [UniProtGOA11a, GOA06]
GO:0055085 - transmembrane transport Inferred by computational analysis [GOA06]
Molecular Function: GO:0022891 - substrate-specific transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA06, Yan07]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [GOA06]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure membrane
transport Putative uncharacterized transport protein

Essentiality data for arnF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: undecaprenyl-phosphate-α-L-Ara4N flippase

Subunit composition of undecaprenyl-phosphate-α-L-Ara4N flippase = [ArnE][ArnF]
         undecaprenyl-phosphate-α-L-Ara4N flippase - ArnE subunit = ArnE (summary available)
         undecaprenyl-phosphate-α-L-Ara4N flippase - ArnF subunit = ArnF (summary available)

Summary:
The ArnE/ArnF undecaprenyl-phosphate-α-L-Ara4N flippase is responsible for transporting undecaprenyl-phosphate-α-L-Ara4N from the cytoplasmic face of the inner membrane to the periplasmic face of the inner membrane.

ArnE and ArnF are both predicted to be integral membrane proteins with 4 transmembrane helices and belong to the drug/metabolite transporter (DMT) superfamily [Yan07]. ArnE and ArnF may act as a heterodimer [Yan07].

Deletion of arnE or arnF results in restoration of polymyxin sensitivity in a polymyxin-resitant pmrAc strain and are defective for transfer of undecaprenyl-phosphate-α-L-Ara4N from the cytoplasmic face of the inner membrane to the periplasmic face and for attachment of L-Ara4N to lipid A [Yan07]. Both ArnE and ArnF are required for polymyxin resistance as expression from a plasmid of one cannot replace a deletion of the other [Yan07].

Expression of the arnBCADTEF operon increased during growth with elevated FeSO4, FeCl3, or ZnSO4 and was dependent upon the BasSR two-component signal transduction system [Hagiwara04, Lee05i].

Citations: [Zhou99b, Nummila95, Groisman01, Trent01a, Gibbons05]

Credits:
Created 01-Aug-2008 by Johnson A , JCVI


Enzymatic reaction of: undecaprenyl-phosphate-α-L-Ara4N flippase


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 3 -> 23
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 36 -> 56
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 77 -> 97
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 101 -> 121
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Chamnongpol02: Chamnongpol S, Dodson W, Cromie MJ, Harris ZL, Groisman EA (2002). "Fe(III)-mediated cellular toxicity." Mol Microbiol 45(3);711-9. PMID: 12139617

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Gibbons05: Gibbons HS, Kalb SR, Cotter RJ, Raetz CR (2005). "Role of Mg2+ and pH in the modification of Salmonella lipid A after endocytosis by macrophage tumour cells." Mol Microbiol 55(2);425-40. PMID: 15659161

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Groisman01: Groisman EA (2001). "The pleiotropic two-component regulatory system PhoP-PhoQ." J Bacteriol 183(6);1835-42. PMID: 11222580

Hagiwara04: Hagiwara D, Yamashino T, Mizuno T (2004). "A Genome-wide view of the Escherichia coli BasS-BasR two-component system implicated in iron-responses." Biosci Biotechnol Biochem 68(8);1758-67. PMID: 15322361

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lee05i: Lee LJ, Barrett JA, Poole RK (2005). "Genome-wide transcriptional response of chemostat-cultured Escherichia coli to zinc." J Bacteriol 187(3);1124-34. PMID: 15659689

Nummila95: Nummila K, Kilpelainen I, Zahringer U, Vaara M, Helander IM (1995). "Lipopolysaccharides of polymyxin B-resistant mutants of Escherichia coli are extensively substituted by 2-aminoethyl pyrophosphate and contain aminoarabinose in lipid A." Mol Microbiol 16(2);271-8. PMID: 7565089

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Trent01a: Trent MS, Ribeiro AA, Doerrler WT, Lin S, Cotter RJ, Raetz CR (2001). "Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm." J Biol Chem 276(46);43132-44. PMID: 11535605

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Yan07: Yan A, Guan Z, Raetz CR (2007). "An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli." J Biol Chem 282(49);36077-89. PMID: 17928292

Zhou99b: Zhou Z, Lin S, Cotter RJ, Raetz CR (1999). "Lipid A modifications characteristic of Salmonella typhimurium are induced by NH4VO3 in Escherichia coli K12. Detection of 4-amino-4-deoxy-L-arabinose, phosphoethanolamine and palmitate." J Biol Chem 274(26);18503-14. PMID: 10373459

Other References Related to Gene Regulation

Froelich06: Froelich JM, Tran K, Wall D (2006). "A pmrA constitutive mutant sensitizes Escherichia coli to deoxycholic acid." J Bacteriol 188(3);1180-3. PMID: 16428424

Ogasawara12: Ogasawara H, Shinohara S, Yamamoto K, Ishihama A (2012). "Novel regulation targets of the metal-response BasS-BasR two-component system of Escherichia coli." Microbiology 158(Pt 6);1482-92. PMID: 22442305


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, biocyc13.