Escherichia coli K-12 substr. MG1655 Enzyme: phosphohistidine phosphatase

Gene: sixA Accession Numbers: G7211 (EcoCyc), b2340, ECK2334

Synonyms: yfcW

Regulation Summary Diagram: ?

Regulation summary diagram for sixA

The sixA gene encodes a phosphohistidine phosphatase that specifically removes the phosphoryl group from the His717 residue of ArcB [Ogino98, Matsubara00]. Under anaerobic growth conditions, this accelerates the derepression of the sdh operon via the ArcB/ArcA phosphorelay in the presence of anaerobic electron acceptors [Matsubara00].

Expression of sixA is dependent on the alternative sigma factor σE [Rezuchova03].

Crystal structures of the free and tungstate-bound forms of SixA have been solved at 2.06 and 1.9 Å resolution [Hamada05].

SixA: "signal inhibitory factor X" [Ogino98]

Locations: cytosol

Map Position: [2,454,349 <- 2,454,834] (52.9 centisomes, 190°)
Length: 486 bp / 161 aa

Molecular Weight of Polypeptide: 17.208 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007720 , DIP:DIP-10885N , EchoBASE:EB3878 , EcoGene:EG14126 , EcoliWiki:b2340 , ModBase:P76502 , OU-Microarray:b2340 , PortEco:sixA , PR:PRO_000023952 , Pride:P76502 , Protein Model Portal:P76502 , RefSeq:NP_416842 , RegulonDB:G7211 , SMR:P76502 , String:511145.b2340 , UniProt:P76502

Relationship Links: InterPro:IN-FAMILY:IPR004449 , InterPro:IN-FAMILY:IPR013078 , InterPro:IN-FAMILY:IPR029033 , PDB:Structure:1UJB , PDB:Structure:1UJC , Pfam:IN-FAMILY:PF00300 , Smart:IN-FAMILY:SM00855

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006464 - cellular protein modification process Inferred from experiment Inferred by computational analysis [GOA01, Hakoshima07]
GO:0016311 - dephosphorylation Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Hakoshima07]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14]
GO:0008969 - phosphohistidine phosphatase activity Inferred from experiment Inferred by computational analysis [GOA01, Hakoshima07]
GO:0004721 - phosphoprotein phosphatase activity Inferred by computational analysis [UniProtGOA11]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm Inferred from experiment Inferred by computational analysis [GOA01, Hakoshima07]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer protein related posttranslational modification
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for sixA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Enzymatic reaction of: phosphohistidine phosphatase

EC Number: 3.1.3.-

ArcB-Phis717 + H2O <=> ArcB + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is irreversible in the direction shown.

Sequence Features

Protein sequence of phosphohistidine phosphatase with features indicated

Feature Class Location Citations Comment
Sequence-Conflict 80
[Yamamoto97a, UniProt10a]
UniProt: (in Ref. 2);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hakoshima07: Hakoshima T, Ichihara H (2007). "Structure of SixA, a histidine protein phosphatase of the ArcB histidine-containing phosphotransfer domain in Escherichia coli." Methods Enzymol 422;288-304. PMID: 17628145

Hamada05: Hamada K, Kato M, Shimizu T, Ihara K, Mizuno T, Hakoshima T (2005). "Crystal structure of the protein histidine phosphatase SixA in the multistep His-Asp phosphorelay." Genes Cells 10(1);1-11. PMID: 15670209

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Matsubara00: Matsubara M, Mizuno T (2000). "The SixA phospho-histidine phosphatase modulates the ArcB phosphorelay signal transduction in Escherichia coli." FEBS Lett 2000;470(2);118-24. PMID: 10734219

Ogino98: Ogino T, Matsubara M, Kato N, Nakamura Y, Mizuno T (1998). "An Escherichia coli protein that exhibits phosphohistidine phosphatase activity towards the HPt domain of the ArcB sensor involved in the multistep His-Asp phosphorelay." Mol Microbiol 1998;27(3);573-85. PMID: 9489669

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Rezuchova03: Rezuchova B, Miticka H, Homerova D, Roberts M, Kormanec J (2003). "New members of the Escherichia coli sigmaE regulon identified by a two-plasmid system." FEMS Microbiol Lett 225(1);1-7. PMID: 12900013

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yamamoto97a: Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4(2);91-113. PMID: 9205837

Other References Related to Gene Regulation

Rhodius05: Rhodius VA, Suh WC, Nonaka G, West J, Gross CA (2005). "Conserved and variable functions of the sigmaE stress response in related genomes." PLoS Biol 4(1);e2. PMID: 16336047

Wade06: Wade JT, Roa DC, Grainger DC, Hurd D, Busby SJ, Struhl K, Nudler E (2006). "Extensive functional overlap between sigma factors in Escherichia coli." Nat Struct Mol Biol 13(9);806-14. PMID: 16892065

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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