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Escherichia coli K-12 substr. MG1655 Polypeptide: CPS-53 (KpLE1) prophage; bactoprenyl glucosyl transferase



Gene: yfdH Accession Numbers: G7220 (EcoCyc), b2351, ECK2345

Regulation Summary Diagram: ?

Regulation summary diagram for yfdH

Summary:
YfdH is an inner membrane protein with two predicted transmembrane helices. The C terminus is located in the cytoplasm [Daley05].

Locations: inner membrane

Map Position: [2,466,236 -> 2,467,156] (53.16 centisomes, 191°)
Length: 921 bp / 306 aa

Molecular Weight of Polypeptide: 34.635 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007763 , DIP:DIP-11997N , EchoBASE:EB3884 , EcoGene:EG14132 , EcoliWiki:b2351 , ModBase:P77293 , OU-Microarray:b2351 , PortEco:yfdH , Protein Model Portal:P77293 , RefSeq:NP_416852 , RegulonDB:G7220 , String:511145.b2351 , UniProt:P77293

Relationship Links: CAZy:IN-FAMILY:GT2 , InterPro:IN-FAMILY:IPR001173 , InterPro:IN-FAMILY:IPR029044 , Pfam:IN-FAMILY:PF00535

In Paralogous Gene Group: 396 (3 members)

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016757 - transferase activity, transferring glycosyl groups Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07a, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: extrachromosomal prophage genes and phage related functions

Essentiality data for yfdH knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 04-Oct-2006 by Keseler I , SRI International


Sequence Features

Protein sequence of CPS-53 (KpLE1) prophage; bactoprenyl glucosyl transferase with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 228 -> 248
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 263 -> 283
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang07a: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Jul 31, 2015, BIOCYC13B.