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Escherichia coli K-12 substr. MG1655 Enzyme: tRNA pseudouridine 65 synthase



Gene: truC Accession Numbers: G7449 (EcoCyc), b2791, ECK2785

Synonyms: yqcB

Regulation Summary Diagram: ?

Summary:
TruC is the pseudouridine synthase that catalyzes formation of pseudouridine at position 65 in tRNAIle1 and tRNAAsp. TruC belongs to the RluA family of RNA pseudouridine synthases [Del01].

A truC null mutant exhibits no obvious growth defect. The conserved active site residue Asp54 is essential for activity [Del01].

Review: [Hamma06]

Locations: cytosol

Map Position: [2,921,024 <- 2,921,806] (62.96 centisomes)
Length: 783 bp / 260 aa

Molecular Weight of Polypeptide: 29.666 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009147 , EchoBASE:EB2963 , EcoGene:EG13171 , EcoliWiki:b2791 , OU-Microarray:b2791 , PortEco:truC , PR:PRO_000024127 , Protein Model Portal:P0AA41 , RefSeq:NP_417271 , RegulonDB:G7449 , SMR:P0AA41 , String:511145.b2791 , UniProt:P0AA41

Relationship Links: InterPro:IN-FAMILY:IPR006145 , InterPro:IN-FAMILY:IPR006224 , InterPro:IN-FAMILY:IPR020103 , Pfam:IN-FAMILY:PF00849 , Prosite:IN-FAMILY:PS01129

In Paralogous Gene Group: 252 (4 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006399 - tRNA metabolic process Inferred from experiment [Del01]
GO:0008033 - tRNA processing Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Del01]
GO:0009451 - RNA modification Inferred from experiment [Del01]
GO:0031119 - tRNA pseudouridine synthesis Inferred from experiment [Del01]
GO:0001522 - pseudouridine synthesis Inferred by computational analysis [GOA01a]
Molecular Function: GO:0009982 - pseudouridine synthase activity Inferred from experiment Inferred by computational analysis [GOA01a, Del01]
GO:0003723 - RNA binding Inferred by computational analysis [GOA01a]
GO:0016853 - isomerase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08]

MultiFun Terms: information transfer RNA related RNA modification

Essentiality data for truC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 13-Mar-2007 by Keseler I , SRI International


Enzymatic reaction of: tRNA pseudouridine synthase (tRNA pseudouridine 65 synthase)

EC Number: 5.4.99.26

a tRNA uridine65 <=> a tRNA pseudouridine65

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Reversibility of this reaction is unspecified.


Sequence Features

Feature Class Location Citations Comment
Mutagenesis-Variant 54
[Del01, UniProt11]
Alternate sequence: D → T; UniProt: Loss of activity.
Active-Site 54
[UniProt10]


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Del01: Del Campo M, Kaya Y, Ofengand J (2001). "Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli." RNA 7(11);1603-15. PMID: 11720289

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hamma06: Hamma T, Ferre-D'Amare AR (2006). "Pseudouridine synthases." Chem Biol 13(11);1125-35. PMID: 17113994

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC14B.