Escherichia coli K-12 substr. MG1655 Polypeptide: toxin of the CptA-CptB toxin-antitoxin system

Gene: cptA Accession Numbers: G7509 (EcoCyc), b2896, ECK2891

Synonyms: ygfX

Regulation Summary Diagram: ?

Regulation summary diagram for cptA

CptA is a membrane-associated toxin that interacts with MreB and FtsZ, inhibiting their polymerization in vitro. CptB is the cognate antitoxin [Masuda12].

Overexpression of CptA causes growth arrest; elongated cells begin to form after 1 h, followed by the formation of lemon-shaped cells. Simultaneous overexpression of CptB neutralizes the effect of CptA overexpression [Masuda12].

CptA has two predicted N-terminal transmembrane domains and a soluble C-terminal domain that is involved in its interaction with MreB and FtsZ [Masuda12].

CptA-CptB was first identified as a potential toxin-antitoxin pair by [Brown03].

CptA: "cytoskeleton polymerization inhibiting toxin" [Masuda12]

Locations: inner membrane

Map Position: [3,038,438 <- 3,038,845] (65.49 centisomes, 236°)
Length: 408 bp / 135 aa

Molecular Weight of Polypeptide: 16.064 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009505 , EchoBASE:EB2884 , EcoGene:EG13074 , EcoliWiki:b2896 , OU-Microarray:b2896 , PortEco:ygfX , Pride:Q46824 , Protein Model Portal:Q46824 , RefSeq:NP_417372 , RegulonDB:G7509 , String:511145.b2896 , UniProt:Q46824

Relationship Links: InterPro:IN-FAMILY:IPR009883 , Pfam:IN-FAMILY:PF07254 , ProDom:IN-FAMILY:PD083358

GO Terms:

Biological Process: GO:0032091 - negative regulation of protein binding Inferred from experiment [Masuda12]
GO:0008360 - regulation of cell shape Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Masuda12]
Cellular Component: GO:0005887 - integral component of plasma membrane Inferred from experiment [Masuda12]
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell processes

Essentiality data for cptA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 19-Jan-2012 by Keseler I , SRI International

Sequence Features

Protein sequence of toxin of the CptA-CptB toxin-antitoxin system with features indicated

Feature Class Location Citations Comment
Protein-Segment 1 -> 96
UniProt: Not required to inhibit FtsZ or MreB polymerization; Sequence Annotation Type: region of interest.
Transmembrane-Region 12 -> 32
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 38 -> 54
UniProt: Helical; Non-Experimental Qualifier: potential.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brown03: Brown JM, Shaw KJ (2003). "A novel family of Escherichia coli toxin-antitoxin gene pairs." J Bacteriol 185(22);6600-8. PMID: 14594833

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Masuda12: Masuda H, Tan Q, Awano N, Yamaguchi Y, Inouye M (2012). "A novel membrane-bound toxin for cell division, CptA (YgfX), inhibits polymerization of cytoskeleton proteins, FtsZ and MreB, in Escherichia coli." FEMS Microbiol Lett 328(2);174-81. PMID: 22239607

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed Oct 7, 2015, biocyc14.