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Escherichia coli K-12 substr. MG1655 Polypeptide: conserved protein



Gene: ygjQ Accession Numbers: G7605 (EcoCyc), b3086, ECK3076

Regulation Summary Diagram: ?

Summary:
An up mutation in the ygjQ ribosome binding site increases fitness for growth on 18-20% cellulosic hydrolysate [Sandoval12].

Locations: inner membrane

Map Position: [3,234,562 -> 3,235,254] (69.72 centisomes)
Length: 693 bp / 230 aa

Molecular Weight of Polypeptide: 25.48 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010142 , EchoBASE:EB2587 , EcoGene:EG12728 , EcoliWiki:b3086 , OU-Microarray:b3086 , PortEco:ygjQ , Pride:P42598 , Protein Model Portal:P42598 , RefSeq:NP_417557 , RegulonDB:G7605 , String:511145.b3086 , UniProt:P42598

Relationship Links: InterPro:IN-FAMILY:IPR003848 , InterPro:IN-FAMILY:IPR014729 , Pfam:IN-FAMILY:PF02698

GO Terms:

Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [DiazMejia09]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

Gene Class: ORFs Conserved-ORFs

Essentiality data for ygjQ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 03-Jul-2012 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 18 -> 35
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Sandoval12: Sandoval NR, Kim JY, Glebes TY, Reeder PJ, Aucoin HR, Warner JR, Gill RT (2012). "Strategy for directing combinatorial genome engineering in Escherichia coli." Proc Natl Acad Sci U S A 109(26);10540-5. PMID: 22689973

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14B.