|Gene:||agaW||Accession Numbers: G7633 (EcoCyc), b3134, ECK3122|
Synonyms: yhaZ, EIIC'Aga, EIIC'GalNAc, Enzyme IIC'Aga, Enzyme IIC'GalNAc
Component of: N-acetyl-D-galactosamine PTS permease (cryptic) (extended summary available)
Sequence analysis predicts that agaW encodes a truncated protein with similarity to the N-terminal region of the of IIC proteins of the PTS Enzymes II specific for mannose [Reizer96c].
E. coli K-12 does not contain the genes encoding Enzymes IIDAga and IIAAga (encoded by agaE and agaF in E. coli strains B and C). In addition the agaW gene, encoding an Enzyme IICAga and agaA encoding a deacetylase have been truncated and thus K-12 is unable to use D-galactosamine (GalN) or N-acetyl-D-galactosamine (GalNAc) as a carbon source [Brinkkotter00].
Locations: inner membrane
|Map Position: [3,278,723 -> 3,279,124] (70.67 centisomes)||Length: 402 bp / 133 aa|
Molecular Weight of Polypeptide: 13.721 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0010299 , EchoBASE:EB2618 , EcoGene:EG12765 , EcoliWiki:b3134 , OU-Microarray:b3134 , PortEco:agaW , Protein Model Portal:P42905 , RefSeq:NP_417603 , RegulonDB:G7633 , UniProt:P42905
In Paralogous Gene Group: 495 (3 members)
|Biological Process:||GO:0006810 - transport
GO:0008643 - carbohydrate transport [UniProtGOA11]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system [UniProtGOA11, GOA01]
|Cellular Component:||GO:0005886 - plasma membrane
GO:0016020 - membrane [UniProtGOA11]
GO:0016021 - integral component of membrane [UniProtGOA11, GOA01]
|MultiFun Terms:||All-Genes → Pseudo-Genes|
|MultiFun → transport → Group Translocators → Phosphotransferase Systems (PEP-dependent PTS)|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB Lennox||Yes||37||Aerobic||7||Yes [Baba06, Comment 1]|
|M9 medium with 1% glycerol||Yes||37||Aerobic||7.2||0.35||Yes [Joyce06, Comment 2]|
|MOPS medium with 0.4% glucose||Yes||37||Aerobic||7.2||0.22||Yes [Baba06, Comment 1]|
Subunit of: N-acetyl-D-galactosamine PTS permease (cryptic)
Subunit composition of
N-acetyl-D-galactosamine PTS permease (cryptic) = [AgaV][AgaW]
N-acetyl-D-galactosamine PTS permease (cryptic) - AgaV subunit = AgaV (summary available)
N-acetyl-D-galactosamine PTS permease (cryptic) - AgaW subunit (pseudogene) = AgaW (summary available)
E. coli contains a cluster of genes (agaZVWEFASYBCDI) responsible for the uptake and metabolism of D-galactosamine (GalN or Gam) and N-acetyl-D-galactosamine (GalNAc or Aga). However, in strain K-12 there is a deletion of the region from agaW' to 'agaA making this organism unable to utilize GalN and GalNAc as sole carbon sources [Brinkkotter00].
AgaVWEF, the cryptic GalNAc PTS permease, belongs to the functional superfamily of the PEP-dependent, sugar transporting phosphotransferase system (PTSsugar). If all of its components were present, AgaVWEF would take up exogenous GalNAc, releasing the phosphate ester into the cell cytoplasm in preparation for metabolism [Postma93, Brinkkotter00].
AgaV is an Enzyme IIBAga. AgaW is a truncated Enzyme IICAga. agaE and agaF encoding Enzymes IIDAga and IIAAga/Gam, respectively, have been deleted. All of these proteins are homologous to the mannose Enzyme II complex proteins (the splinter group enzymes) [Reizer96c, Brinkkotter00].
The aga operon (agaZVW' 'ASYBCDI) also encodes other enzymes for the metabolism of GalNAc and GalN: GalNAc-6-phosphate deacetylase (AgaA), a putative isomerase (AgaS), a tagatose-bisphosphate aldolase (AgaYZ) and a GalN-6-phosphate deaminase (AgaI). The truncated agaA gene in E. coli K-12 is inactive. The agaR gene, encoding a transcriptional repressor of the aga operon, precedes and is divergently transcribed from the aga operon [Brinkkotter00, Ray04]
|Conserved-Region||1 -> 133|
|Transmembrane-Region||3 -> 23|
|Transmembrane-Region||33 -> 53|
|Transmembrane-Region||66 -> 86|
|Transmembrane-Region||94 -> 114|
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
Brinkkotter00: Brinkkotter A, Kloss H, Alpert C, Lengeler JW (2000). "Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli." Mol Microbiol 2000;37(1);125-35. PMID: 10931310
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Ray04: Ray WK, Larson TJ (2004). "Application of AgaR repressor and dominant repressor variants for verification of a gene cluster involved in N-acetylgalactosamine metabolism in Escherichia coli K-12." Mol Microbiol 51(3);813-26. PMID: 14731281
Reizer96c: Reizer J, Ramseier TM, Reizer A, Charbit A, Saier MH (1996). "Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Microbiology 1996;142 ( Pt 2);231-50. PMID: 8932697
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