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Escherichia coli K-12 substr. MG1655 Polypeptide: putative fimbrial protein



Gene: yraK Accession Numbers: G7640 (EcoCyc), b3145, ECK3133

Regulation Summary Diagram: ?

Summary:
yraHIJK is a putative chaperone-usher fimbrial operon in E.coli K-12 [Korea10]. The operon is cryptic under normal laboratory conditions but when constitutive expression is induced in a strain that lacks the type 1 fimbrial complex the yra operon promotes adhesion to eukaryotic epithelial cells (T4 bladder cells) [Korea10]. Expression of the yra operon is regulated by the global regulators H-NS and CRP cAMP [Korea10].

Sequence similarity suggests that YraK may contain β-barrel structure(s) [Zhai02].

Citations: [Korea11]

Locations: extracellular space, pilus

Map Position: [3,289,363 -> 3,290,454] (70.9 centisomes)
Length: 1092 bp / 363 aa

Molecular Weight of Polypeptide: 38.535 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010335 , EchoBASE:EB2629 , EcoGene:EG12776 , EcoliWiki:b3145 , ModBase:P43319 , OU-Microarray:b3145 , PortEco:yraK , Protein Model Portal:P43319 , RefSeq:NP_417614 , RegulonDB:G7640 , SMR:P43319 , String:511145.b3145 , UniProt:P43319

Relationship Links: EcoO157Cyc:Homolog:Z4501 , EcoO157Cyc:Homolog:Z4501-MONOMER , EcoO157Cyc:Homolog:Z4504 , EcoO157Cyc:Homolog:Z4504-MONOMER , EcoO157Cyc:Homolog:Z4966 , EcoO157Cyc:Homolog:Z4966-MONOMER , EcoO157Cyc:Homolog:Z5220 , EcoO157Cyc:Homolog:Z5220-MONOMER , EcoO157Cyc:Homolog:Z5221 , EcoO157Cyc:Homolog:Z5221-MONOMER , InterPro:IN-FAMILY:IPR000259 , InterPro:IN-FAMILY:IPR008966 , Pfam:IN-FAMILY:PF00419 , Prosite:IN-FAMILY:PS51257

In Paralogous Gene Group: 43 (37 members)

GO Terms:

Biological Process: GO:0043709 - cell adhesion involved in single-species biofilm formation Inferred from experiment [Korea10]
GO:0007155 - cell adhesion Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005576 - extracellular region Inferred by computational analysis [DiazMejia09]
GO:0009289 - pilus Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA01a, Korea10]

MultiFun Terms: cell structure pilus

Essentiality data for yraK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 13-Apr-2010 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 20
[UniProt10]
UniProt: Non-Experimental Qualifier: potential;
Chain 21 -> 363
[UniProt09]
UniProt: Uncharacterized fimbrial-like protein yraK;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Korea10: Korea CG, Badouraly R, Prevost MC, Ghigo JM, Beloin C (2010). "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities." Environ Microbiol 12(7):1957-77. PMID: 20345943

Korea11: Korea CG, Ghigo JM, Beloin C (2011). "The sweet connection: Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli fimbriae form a versatile arsenal of sugar-binding lectins potentially involved in surface-colonisation and tissue tropism." Bioessays 33(4);300-11. PMID: 21344461

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Zhai02: Zhai Y, Saier MH (2002). "The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes." Protein Sci 11(9);2196-207. PMID: 12192075


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14A.