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Escherichia coli K-12 substr. MG1655 Polypeptide: outer membrane lipoprotein - activator of PBP1A activity



Gene: lpoA Accession Numbers: G7642 (EcoCyc), b3147, ECK3135

Synonyms: yraM

Regulation Summary Diagram: ?

Summary:
LpoA is an outer membrane lipoprotein that forms a complex with, and is essential for the in vivo function of, penicillin binding protein 1A (PBP1A) [ParadisBleau10]. LpoA stimulates the transpeptidase activity of PBP1A in vitro [Typas10].

An lpoA- mutant is synthetically lethal in a PBP1B defective strain and this phenotype can be corrected by the expression of lpoA from a plasmid [ParadisBleau10]. An lpoA-lpoB- mutant displays a similar terminal phenotype to PBP1A- PBP1B- cells [ParadisBleau10]. Peptidoglycan isolated from an lpoA-lpoB- mutant prior to lysis shows a substantial decrease in peptidoglycan cross-linking and and an increase in pentapeptide containing muropeptides - a similar biochemistry is observed in the peptidoglycan of PBP1A- PBP1B- cells [ParadisBleau10]. Pull-down assays using tagged LpoA indicate that it specifically associates with PBP1A; an in vivo stoichiometry of 1:1 has been estimated [ParadisBleau10].

LpoA localises independently of PBP1A but like PBP1A, it concentrates at the sidewall of elongating cells [Typas10].

Locations: periplasmic space, outer membrane

Map Position: [3,291,422 -> 3,293,458] (70.94 centisomes)
Length: 2037 bp / 678 aa

Molecular Weight of Polypeptide: 72.825 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010343 , DIP:DIP-12897N , EchoBASE:EB2631 , EcoGene:EG12778 , EcoliWiki:b3147 , Mint:MINT-1258091 , ModBase:P45464 , OU-Microarray:b3147 , PortEco:yraM , Pride:P45464 , Protein Model Portal:P45464 , RefSeq:NP_417616 , RegulonDB:G7642 , SMR:P45464 , String:511145.b3147 , UniProt:P45464

Relationship Links: InterPro:IN-FAMILY:IPR007443 , InterPro:IN-FAMILY:IPR016026 , InterPro:IN-FAMILY:IPR028082 , Pfam:IN-FAMILY:PF04348 , Prosite:IN-FAMILY:PS51257

In Paralogous Gene Group: 499 (2 members)

GO Terms:

Biological Process: GO:0009252 - peptidoglycan biosynthetic process Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA06, Typas10, ParadisBleau10]
GO:0050790 - regulation of catalytic activity Inferred by computational analysis Inferred from experiment [Typas10, GOA06]
GO:0008360 - regulation of cell shape Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Typas10, ParadisBleau10, Butland05, Arifuzzaman06]
GO:0019899 - enzyme binding Inferred from experiment [Typas10, ParadisBleau10]
GO:0030234 - enzyme regulator activity Inferred from experiment Inferred by computational analysis [GOA06, Typas10]
GO:0004553 - hydrolase activity, hydrolyzing O-glycosyl compounds Inferred by computational analysis [GOA01]
Cellular Component: GO:0009279 - cell outer membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, Typas10, ParadisBleau10]
GO:0031241 - periplasmic side of cell outer membrane Inferred from experiment Inferred by computational analysis [GOA06, Typas10]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0042597 - periplasmic space Inferred by computational analysis [GOA01]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of macromolecules (cellular constituents) murein (peptidoglycan)

Essentiality data for lpoA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 06-Jan-2011 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Common Name Citations Comment
Signal-Sequence 1 -> 26  
[UniProt11]
UniProt: Non-Experimental Qualifier: potential.
Signal-Sequence 1 -> 33  
[ParadisBleau10]
 
Signal-Sequence 24 -> 27 lipo-box
[ParadisBleau10]
 
Lipid-Binding-Site 27  
[UniProt11]
UniProt: N-palmitoyl cysteine; Non-Experimental Qualifier: potential.
Chain 27 -> 678  
[UniProt11]
UniProt: Penicillin-binding protein activator LpoA.


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

ParadisBleau10: Paradis-Bleau C, Markovski M, Uehara T, Lupoli TJ, Walker S, Kahne DE, Bernhardt TG (2010). "Lipoprotein cofactors located in the outer membrane activate bacterial cell wall polymerases." Cell 143(7);1110-20. PMID: 21183074

Typas10: Typas A, Banzhaf M, van den Berg van Saparoea B, Verheul J, Biboy J, Nichols RJ, Zietek M, Beilharz K, Kannenberg K, von Rechenberg M, Breukink E, den Blaauwen T, Gross CA, Vollmer W (2010). "Regulation of peptidoglycan synthesis by outer-membrane proteins." Cell 143(7);1097-109. PMID: 21183073

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.