Escherichia coli K-12 substr. MG1655 Polypeptide: Blc outer membrane lipoprotein (lipocalin)

Gene: blc Accession Numbers: G7837 (EcoCyc), b4149, ECK4145

Synonyms: yjeL

Regulation Summary Diagram: ?

Regulation summary diagram for blc

The Blc protein is a globomycin-sensitive outer membrane lipoprotein [Bishop95]. Expression of the blc gene is induced at the beginning of stationary phase [Bishop95]. The Blc crystal structure has been solved at 1.8 A resolution [Campanacci04].

Structural analysis of the purified Blc protein suggest a possible role for the outer membrane lipoprotein Blc in membrane repair or maintenance requiring lipid storage or transport [Campanacci04].

Citations: [Bishop00]

Locations: outer membrane

Map Position: [4,375,212 <- 4,375,745] (94.3 centisomes, 339°)
Length: 534 bp / 177 aa

Molecular Weight of Polypeptide: 19.852 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013590 , DIP:DIP-35862N , EchoBASE:EB2367 , EcoGene:EG12474 , EcoliWiki:b4149 , ModBase:P0A901 , OU-Microarray:b4149 , PortEco:blc , PR:PRO_000022228 , Pride:P0A901 , Protein Model Portal:P0A901 , RefSeq:NP_418573 , RegulonDB:G7837 , SMR:P0A901 , String:511145.b4149 , UniProt:P0A901

Relationship Links: InterPro:IN-FAMILY:IPR000566 , InterPro:IN-FAMILY:IPR002446 , InterPro:IN-FAMILY:IPR011038 , InterPro:IN-FAMILY:IPR012674 , InterPro:IN-FAMILY:IPR022271 , InterPro:IN-FAMILY:IPR022272 , PDB:Structure:1QWD , PDB:Structure:2ACO , PDB:Structure:3MBT , Pfam:IN-FAMILY:PF08212 , Prints:IN-FAMILY:PR01171 , Prosite:IN-FAMILY:PS00213 , Prosite:IN-FAMILY:PS51257

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0006810 - transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
GO:0008289 - lipid binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0009279 - cell outer membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Bishop95]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]


Essentiality data for blc knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Sequence Features

Protein sequence of Blc outer membrane lipoprotein (lipocalin) with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 18
UniProt: Non-Experimental Qualifier: probable;
Lipid-Binding-Site 19
UniProt: N-palmitoyl cysteine; Non-Experimental Qualifier: probable;
Chain 19 -> 177
UniProt: Outer membrane lipoprotein blc;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bishop00: Bishop RE (2000). "The bacterial lipocalins." Biochim Biophys Acta 1482(1-2);73-83. PMID: 11058749

Bishop95: Bishop RE, Penfold SS, Frost LS, Holtje JV, Weiner JH (1995). "Stationary phase expression of a novel Escherichia coli outer membrane lipoprotein and its relationship with mammalian apolipoprotein D. Implications for the origin of lipocalins." J Biol Chem 1995;270(39);23097-103. PMID: 7559452

Campanacci04: Campanacci V, Nurizzo D, Spinelli S, Valencia C, Tegoni M, Cambillau C (2004). "The crystal structure of the Escherichia coli lipocalin Blc suggests a possible role in phospholipid binding." FEBS Lett 562(1-3);183-8. PMID: 15044022

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed Oct 7, 2015, BIOCYC14A.