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Escherichia coli K-12 substr. MG1655 Enzyme: epoxyqueuosine reductase



Gene: queG Accession Numbers: G7843 (EcoCyc), b4166, ECK4162

Synonyms: yjeS

Regulation Summary Diagram: ?

Summary:
Epoxyqueuosine reductase catalyzes the final step in the de novo synthesis of queuosine, the anticodon loop modification found in tRNA(Asp), tRNA(Asn), tRNA(His), and tRNA(Tyr) [Miles11].

The enzyme was identified by screening the Keio collection of single-gene knockouts for presence of epoxyqueuosine (oQ) and lack of queuosine (Q) in tRNAs by tandem UV-visible spectrophotometry and mass spectroscopy [Miles11].

Gene Citations: [Kitagawa96]

Locations: cytosol

Map Position: [4,390,951 <- 4,392,090] (94.64 centisomes)
Length: 1140 bp / 379 aa

Molecular Weight of Polypeptide: 43.073 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013641 , EchoBASE:EB2374 , EcoGene:EG12481 , EcoliWiki:b4166 , ModBase:P39288 , OU-Microarray:b4166 , PortEco:yjeS , Pride:P39288 , Protein Model Portal:P39288 , RefSeq:NP_418587 , RegulonDB:G7843 , SMR:P39288 , String:511145.b4166 , UniProt:P39288

Relationship Links: InterPro:IN-FAMILY:IPR004453 , InterPro:IN-FAMILY:IPR009051 , InterPro:IN-FAMILY:IPR013542 , InterPro:IN-FAMILY:IPR017896 , InterPro:IN-FAMILY:IPR017900 , Panther:IN-FAMILY:PTHR30002:SF0 , Pfam:IN-FAMILY:PF08331 , Prosite:IN-FAMILY:PS00198 , Prosite:IN-FAMILY:PS51379

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0008616 - queuosine biosynthetic process Inferred from experiment Inferred by computational analysis [UniProtGOA12, UniProtGOA11, GOA06, GOA01, Miles11]
GO:0008033 - tRNA processing Inferred by computational analysis [UniProtGOA11]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0052693 - epoxyqueuosine reductase activity Inferred from experiment Inferred by computational analysis [GOA06, Miles11]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]
GO:0051536 - iron-sulfur cluster binding Inferred by computational analysis [UniProtGOA11, GOA06, GOA01]
GO:0051539 - 4 iron, 4 sulfur cluster binding Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related RNA modification

Essentiality data for queG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 25-Apr-2011 by Keseler I , SRI International


Enzymatic reaction of: epoxyqueuosine reductase

EC Number: 1.17.99.-

a queuosine34 in tRNA + an oxidized electron acceptor + H2O <=> an epoxyqueuosine34 in tRNA + a reduced electron acceptor

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the opposite direction.

In Pathways: queuosine biosynthesis


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 181 -> 213
[UniProt09]
UniProt: 4Fe-4S ferredoxin-type;
Metal-Binding-Site 193
[UniProt10a]
UniProt: Iron-sulfur 1 (4Fe-4S); Non-Experimental Qualifier: potential;
Metal-Binding-Site 196
[UniProt10a]
UniProt: Iron-sulfur 1 (4Fe-4S); Non-Experimental Qualifier: potential;
Metal-Binding-Site 199
[UniProt10a]
UniProt: Iron-sulfur 1 (4Fe-4S); Non-Experimental Qualifier: potential;
Metal-Binding-Site 203
[UniProt10a]
UniProt: Iron-sulfur 2; Non-Experimental Qualifier: potential;
Metal-Binding-Site 246
[UniProt10a]
UniProt: Iron-sulfur 2; Non-Experimental Qualifier: potential;
Metal-Binding-Site 249
[UniProt10a]
UniProt: Iron-sulfur 2; Non-Experimental Qualifier: potential;
Metal-Binding-Site 253
[UniProt10a]
UniProt: Iron-sulfur 1 (4Fe-4S); Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kitagawa96: Kitagawa R, Mitsuki H, Okazaki T, Ogawa T (1996). "A novel DnaA protein-binding site at 94.7 min on the Escherichia coli chromosome." Mol Microbiol 1996;19(5);1137-47. PMID: 8830270

Miles11: Miles ZD, McCarty RM, Molnar G, Bandarian V (2011). "Discovery of epoxyqueuosine (oQ) reductase reveals parallels between halorespiration and tRNA modification." Proc Natl Acad Sci U S A 108(18);7368-72. PMID: 21502530

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, biocyc14.