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Escherichia coli K-12 substr. MG1655 Polypeptide: glutamine ABC transporter - ATP binding subunit

Gene: glnQ Accession Numbers: EG10389 (EcoCyc), b0809, ECK0798

Regulation Summary Diagram: ?

Regulation summary diagram for glnQ

Component of: glutamine ABC transporter (summary available)

Gene Citations: [Nohno87, Nohno86]

Locations: cytosol, inner membrane

Map Position: [844,964 <- 845,686] (18.21 centisomes, 66°)
Length: 723 bp / 240 aa

Molecular Weight of Polypeptide: 26.731 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002764 , CGSC:18268 , DIP:DIP-9786N , EchoBASE:EB0384 , EcoGene:EG10389 , EcoliWiki:b0809 , ModBase:P10346 , OU-Microarray:b0809 , PortEco:glnQ , PR:PRO_000022791 , Pride:P10346 , Protein Model Portal:P10346 , RefSeq:NP_415330 , RegulonDB:EG10389 , SMR:P10346 , String:511145.b0809 , Swiss-Model:P10346 , UniProt:P10346

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for glnQ

GO Terms:

Biological Process: GO:0003333 - amino acid transmembrane transport Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0015424 - amino acid-transporting ATPase activity Inferred by computational analysis [GOA01a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Stenberg05]
GO:0005829 - cytosol
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids glutamine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for glnQ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: glutamine ABC transporter

Subunit composition of glutamine ABC transporter = [GlnQ]2[GlnP]2[GlnH]
         glutamine ABC transporter - ATP binding subunit = GlnQ
         glutamine ABC transporter - membrane subunit = GlnP
         glutamine ABC transporter - periplasmic binding protein = GlnH

The GlnHPQ high-affinity glutamine transport system is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, GlnH is the periplasmic glutamine-binding protein, GlnQ is the ATP-binding component, and GlnP is the membrane component of the ABC transporter. Mutation of glnP results in the impaired ability to transport glutamine as well as the inability to utilized glutamine as a sole source of carbon [Masters81, Nohno86]. Expression of the cloned glnHPQ genes on a plasmid vector restored the glnH, glnP and glnQ mutants' abilities to transport glutamine and utilize glutamine as a sole carbon source [Nohno86].

Enzymatic reaction of: transport of L-glutamine (glutamine ABC transporter)

Synonyms: Transport of L-glutamine

Transport reaction diagram for transport of L-glutamine

Sequence Features

Protein sequence of glutamine ABC transporter - ATP binding subunit with features indicated

Feature Class Location Citations Comment
Conserved-Region 2 -> 236
UniProt: ABC transporter;
Nucleotide-Phosphate-Binding-Region 34 -> 41
UniProt: ATP; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b0809 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10389; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Masters81: Masters PS, Hong JS (1981). "Genetics of the glutamine transport system in Escherichia coli." J Bacteriol 147(3);805-19. PMID: 6115851

Nohno86: Nohno T, Saito T, Hong JS (1986). "Cloning and complete nucleotide sequence of the Escherichia coli glutamine permease operon (glnHPQ)." Mol Gen Genet 1986;205(2);260-9. PMID: 3027504

Nohno87: Nohno T, Saito T (1987). "Two transcriptional start sites found in the promoter region of Escherichia coli glutamine permease operon, glnHPQ." Nucleic Acids Res 1987;15(6);2777. PMID: 3550701

Stenberg05: Stenberg F, Chovanec P, Maslen SL, Robinson CV, Ilag LL, von Heijne G, Daley DO (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280(41);34409-19. PMID: 16079137

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Other References Related to Gene Regulation

ClaverieMartin91: Claverie-Martin F, Magasanik B (1991). "Role of integration host factor in the regulation of the glnHp2 promoter of Escherichia coli." Proc Natl Acad Sci U S A 1991;88(5);1631-5. PMID: 2000372

ClaverieMartin92: Claverie-Martin F, Magasanik B (1992). "Positive and negative effects of DNA bending on activation of transcription from a distant site." J Mol Biol 227(4);996-1008. PMID: 1433305

Freundlich92: Freundlich M, Ramani N, Mathew E, Sirko A, Tsui P (1992). "The role of integration host factor in gene expression in Escherichia coli." Mol Microbiol 6(18);2557-63. PMID: 1447969

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615

Xu95: Xu J, Johnson RC (1995). "Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli." J Bacteriol 177(4);938-47. PMID: 7860604

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sun Aug 30, 2015, biocyc11.