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Escherichia coli K-12 substr. MG1655 Polypeptide: GlvC subunit of predicted PTS permease (pseudogene)



Gene: glvC Accession Numbers: EG11710 (EcoCyc), b3683, ECK3675

Synonyms: ptiC, yidO

Regulation Summary Diagram: ?

Component of: predicted PTS permease GlvCB (cryptic) (extended summary available)

Summary:
Expression of glvC is decreased in a luxS deletion mutant during growth in LB (no glucose) [Wang05a].

glvC is a pseudogene candidate in E. coli K-12 [Homma02].

Citations: [Wolfe05, Kricker87]

Locations: inner membrane

Map Position: [3,860,520 <- 3,861,626] (83.21 centisomes)
Length: 1107 bp / 368 aa

Molecular Weight of Polypeptide: 39.692 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012043 , EchoBASE:EB1661 , EcoGene:EG11710 , EcoliWiki:b3683 , OU-Microarray:b3683 , PortEco:glvC , Pride:P31452 , Protein Model Portal:P31452 , RefSeq:YP_026236 , RegulonDB:EG11710 , SMR:P31452 , String:316407.glvC , UniProt:P31452

Relationship Links: InterPro:IN-FAMILY:IPR003352 , InterPro:IN-FAMILY:IPR004719 , InterPro:IN-FAMILY:IPR010975 , InterPro:IN-FAMILY:IPR013013 , Pfam:IN-FAMILY:PF02378 , Prosite:IN-FAMILY:PS51103

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008643 - carbohydrate transport Inferred by computational analysis Author statement [Reizer94a, UniProtGOA11]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Interrupted-Genes
MultiFun transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for glvC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: predicted PTS permease GlvCB (cryptic)

Synonyms: EIIBCGlv

Subunit composition of predicted PTS permease GlvCB (cryptic) = [GlvC][GlvB]
         GlvC subunit of predicted PTS permease (pseudogene) = GlvC (summary available)
         GlvB subunit of predicted PTS permease (pseudogene) = GlvB (summary available)

Summary:
GlvCB, a predicted arbutin specific PTS permease [Riley06], belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS) [Reizer94a]. The PTS transports and simultaneously phosphorylates its sugar substrates in a process called group translocation (reviewed by [Postma93]).

GlvC has sequence similarity to the IIC domains of the glucose and N-acetylglucosamine Enzymes II in E. coli. The GlvB amino acid sequence shows similarity with the IIB domain of various Enzyme II proteins and contains a conserved cysteine residue (Cys92). The glv operon (glvCBG) may be cryptic in wild type E. coli K-12 [Reizer94a].

glvC and glvB are pseudogene candidates in E. coli K-12.The genome of E. coli O157 contains a complete glvC ortholog [Homma02].

Please note that the presence of a GlvCB complex in E. coli K-12 is hypothetical.

Citations: [Kricker87]

Credits:
Revised 29-Apr-2014 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 368
[UniProt09]
UniProt: PTS EIIC type-1;
Transmembrane-Region 12 -> 32
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 60 -> 80
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 87 -> 107
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 130 -> 150
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 174 -> 194
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 199 -> 221
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 225 -> 245
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 277 -> 297
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 307 -> 327
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 329 -> 349
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
10/20/97 Gene b3683 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11710; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Homma02: Homma K, Fukuchi S, Kawabata T, Ota M, Nishikawa K (2002). "A systematic investigation identifies a significant number of probable pseudogenes in the Escherichia coli genome." Gene 294(1-2);25-33. PMID: 12234664

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kricker87: Kricker M, Hall BG (1987). "Biochemical genetics of the cryptic gene system for cellobiose utilization in Escherichia coli K12." Genetics 115(3);419-29. PMID: 3552873

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer94a: Reizer J, Michotey V, Reizer A, Saier MH (1994). "Novel phosphotransferase system genes revealed by bacterial genome analysis: unique, putative fructose- and glucoside-specific systems." Protein Sci 3(3);440-50. PMID: 8019415

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Wang05a: Wang L, Li J, March JC, Valdes JJ, Bentley WE (2005). "luxS-dependent gene regulation in Escherichia coli K-12 revealed by genomic expression profiling." J Bacteriol 187(24);8350-60. PMID: 16321939

Wolfe05: Wolfe AJ (2005). "The acetate switch." Microbiol Mol Biol Rev 69(1);12-50. PMID: 15755952


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.