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Escherichia coli K-12 substr. MG1655 Polypeptide: 3-phenylpropionate dioxygenase, predicted ferredoxin subunit



Gene: hcaC Accession Numbers: G7334 (EcoCyc), b2540, ECK2537

Synonyms: yfhW, hcaa3, phdB

Regulation Summary Diagram: ?

Component of: 3-phenylpropionate dioxygenase system (summary available)

Summary:
Based on sequence similarity, HcaC is thought to encode the ferredoxin component of the 3-phenylpropionate dioxygenase system [Diaz98]. Partially purified HcaC protein was shown to contain a [2Fe-2S] iron-sulfur cluster that can be reduced by dithionite, but not ascorbate, identifying it as a low-potential Rieske iron-sulfur cluster protein [Boxhammer08]. 3-phenylpropionate dioxygenase has not been biochemically characterized.

E. coli is able to utilize certain aromatic acids as carbon and energy sources. A meta-cleavage pathway involving HcaC is used for the catabolism of 3-phenylpropionate [Burlingame83, Diaz98].

HcaC: "hydroxycinnamic acid" [Diaz98]

Gene Citations: [Turlin05]

Map Position: [2,668,930 -> 2,669,250] (57.52 centisomes)
Length: 321 bp / 106 aa

Molecular Weight of Polypeptide: 11.329 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008359 , EchoBASE:EB3231 , EcoGene:EG13458 , EcoliWiki:b2540 , ModBase:P0ABW0 , OU-Microarray:b2540 , PortEco:hcaC , PR:PRO_000022859 , Protein Model Portal:P0ABW0 , RefSeq:NP_417035 , RegulonDB:G7334 , SMR:P0ABW0 , String:511145.b2540 , Swiss-Model:P0ABW0 , UniProt:P0ABW0

Relationship Links: InterPro:IN-FAMILY:IPR017941 , InterPro:IN-FAMILY:IPR023739 , Pfam:IN-FAMILY:PF00355 , Prosite:IN-FAMILY:PS51296

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0019380 - 3-phenylpropionate catabolic process Inferred from experiment Inferred by computational analysis [UniProtGOA12, Burlingame86]
GO:0019439 - aromatic compound catabolic process Inferred by computational analysis [UniProtGOA11a, GOA06]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0051536 - iron-sulfur cluster binding Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Boxhammer08]
GO:0051537 - 2 iron, 2 sulfur cluster binding Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Boxhammer08]
GO:0008695 - 3-phenylpropionate dioxygenase activity Inferred by computational analysis [GOA06]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [GOA01a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: metabolism carbon utilization carbon compounds

Essentiality data for hcaC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 3]

Credits:
Last-Curated ? 27-Oct-2011 by Keseler I , SRI International


Subunit of: 3-phenylpropionate dioxygenase system

Synonyms: 3-phenylpropionate dioxygenase complex

Subunit composition of 3-phenylpropionate dioxygenase system = [HcaC][HcaD][HcaF][HcaE]
         3-phenylpropionate dioxygenase, predicted ferredoxin subunit = HcaC (summary available)
         3-phenylpropionate dioxygenase, predicted ferredoxin reductase subunit = HcaD (summary available)
         3-phenylpropionate dioxygenase, β subunit = HcaF (summary available)
         3-phenylpropionate dioxygenase, α subunit = HcaE (summary available)

Summary:
The 3-phenylpropionate dioxygenase system is predicted to be encoded by the hcaCDEF genes [Diaz98]. 3-phenylpropionate dioxygenase has not been biochemically characterized.

Credits:
Last-Curated ? 27-Oct-2011 by Keseler I , SRI International


Enzymatic reaction of: cinnamate dioxygenase (3-phenylpropionate dioxygenase system)

EC Number: 1.14.12.19

trans-cinnamate + NADH + oxygen + H+ <=> (2E)-3-(5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

In Pathways: cinnamate and 3-hydroxycinnamate degradation to 2-oxopent-4-enoate


Enzymatic reaction of: 3-phenylpropionate dioxygenase

EC Number: 1.14.12.19

3-phenylpropanoate + NADH + oxygen + H+ <=> 3-(5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

In Pathways: 3-phenylpropionate and 3-(3-hydroxyphenyl)propionate degradation , 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-oxopent-4-enoate


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 4 -> 99
[UniProt09]
UniProt: Rieske;
Metal-Binding-Site 42
[UniProt10]
UniProt: Iron-sulfur (2Fe-2S); Non-Experimental Qualifier: potential;
Metal-Binding-Site 44
[UniProt10]
UniProt: Iron-sulfur (2Fe-2S); via pros nitrogen; Non-Experimental Qualifier: potential;
Metal-Binding-Site 62
[UniProt10]
UniProt: Iron-sulfur (2Fe-2S); Non-Experimental Qualifier: potential;
Metal-Binding-Site 65
[UniProt10]
UniProt: Iron-sulfur (2Fe-2S); via pros nitrogen; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Boxhammer08: Boxhammer S, Glaser S, Kuhl A, Wagner AK, Schmidt CL (2008). "Characterization of the recombinant Rieske [2Fe-2S] proteins HcaC and YeaW from E. coli." Biometals 21(4):459-67. PMID: 18286376

Burlingame83: Burlingame R, Chapman PJ (1983). "Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli." J Bacteriol 1983;155(1);113-21. PMID: 6345502

Burlingame86: Burlingame RP, Wyman L, Chapman PJ (1986). "Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation." J Bacteriol 1986;168(1);55-64. PMID: 3531186

Diaz98: Diaz E, Ferrandez A, Garcia JL (1998). "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12." J Bacteriol 1998;180(11);2915-23. PMID: 9603882

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Turlin05: Turlin E, Sismeiro O, Le Caer JP, Labas V, Danchin A, Biville F (2005). "3-phenylpropionate catabolism and the Escherichia coli oxidative stress response." Res Microbiol 156(3);312-21. PMID: 15808934

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Other References Related to Gene Regulation

Turlin01: Turlin E, Perrotte-piquemal M, Danchin A, Biville F (2001). "Regulation of the early steps of 3-phenylpropionate catabolism in Escherichia coli." J Mol Microbiol Biotechnol 3(1);127-33. PMID: 11200225


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.