Escherichia coli K-12 substr. MG1655 Polypeptide: histidine ABC transporter - periplasmic binding protein

Gene: hisJ Accession Numbers: EG12124 (EcoCyc), b2309, ECK2303

Regulation Summary Diagram: ?

Regulation summary diagram for hisJ

Component of: histidine ABC transporter (extended summary available)

Gene Citations: [Zimmer00]

Locations: periplasmic space

Map Position: [2,424,028 <- 2,424,810] (52.25 centisomes, 188°)
Length: 783 bp / 260 aa

Molecular Weight of Polypeptide: 28.483 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007617 , CGSC:627 , DIP:DIP-9908N , EchoBASE:EB2045 , EcoGene:EG12124 , EcoliWiki:b2309 , ModBase:P0AEU0 , OU-Microarray:b2309 , PortEco:hisJ , PR:PRO_000022899 , Pride:P0AEU0 , Protein Model Portal:P0AEU0 , RefSeq:NP_416812 , RegulonDB:EG12124 , SMR:P0AEU0 , String:511145.b2309 , Swiss-Model:P0AEU0 , UniProt:P0AEU0

Relationship Links: InterPro:IN-FAMILY:IPR001638 , InterPro:IN-FAMILY:IPR005768 , InterPro:IN-FAMILY:IPR018313 , PDB:Structure:1HSL , Pfam:IN-FAMILY:PF00497 , Prosite:IN-FAMILY:PS01039 , Smart:IN-FAMILY:SM00062

In Paralogous Gene Group: 172 (5 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for hisJ

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [GOA01, DiazMejia09, LopezCampistrou05]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids histidine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Essentiality data for hisJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: histidine ABC transporter

Subunit composition of histidine ABC transporter = [HisP]2[HisM][HisQ][HisJ]
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - ATP binding subunit = HisP
         lysine/arginine/ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisM
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisQ
         histidine ABC transporter - periplasmic binding protein = HisJ

HisPMQJ is an ATP-dependent histidine transport system that is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, HisJ is the periplasmic histidine-binding protein, HisQ and HisM are the integral membrane components, and HisP is the ATP-binding component of the ABC transport complex [Liu98d]. Although the transport properties of the His complex in E. coli have not yet been characterized, extensive investigations on the orthologous proteins in Salmonella typhimurium have been reported. The His complex of Salmonella typhimurium was purified and reconstituted into ATP-encapsulated proteoliposomes, and histidine transport activity was observed [Liu97d]. His-mediated transport activity is completely dependent on the presence of all four protein components and on the internal ATP concentration, with apparent Km of 8 mM for ATP [Liu97d, Bishop89]. The transport activity is also affected by pH, temperature, and salt concentration [Liu97d]. Transport is irreversible and accumulation reaches a plateau at which point transport ceases [Liu97d]. The transport complex is inhibited by ADP and by high concentrations of internal histidine [Liu97d].

Enzymatic reaction of: histidine ABC transporter

Transport reaction diagram for histidine ABC transporter

Sequence Features

Protein sequence of histidine ABC transporter - periplasmic binding protein with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 22
[Wilkins98, Link97, UniProt11]
Sequence-Conflict 21
[Joshi96, UniProt15]
UniProt: (in Ref. 1; AAA85769).
Chain 23 -> 260
UniProt: Histidine-binding periplasmic protein;
Disulfide-Bond-Site 60, 67
UniProt: Non-Experimental Qualifier: by similarity;
Sequence-Conflict 188
[Joshi96, UniProt15]
UniProt: (in Ref. 1; AAA85769).

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b2309 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12124; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bishop89: Bishop L, Agbayani R, Ambudkar SV, Maloney PC, Ames GF (1989). "Reconstitution of a bacterial periplasmic permease in proteoliposomes and demonstration of ATP hydrolysis concomitant with transport." Proc Natl Acad Sci U S A 1989;86(18);6953-7. PMID: 2674940

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joshi96: Joshi A., Ames G.F.-L. (1996). Data submission to EMBL/GenBank/DDBJ databases on 1996-01.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Link97: Link AJ, Robison K, Church GM (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18(8);1259-313. PMID: 9298646

Liu97d: Liu CE, Ames GF (1997). "Characterization of transport through the periplasmic histidine permease using proteoliposomes reconstituted by dialysis." J Biol Chem 1997;272(2);859-66. PMID: 8995374

Liu98d: Liu PQ, Ames GF (1998). "In vitro disassembly and reassembly of an ABC transporter, the histidine permease." Proc Natl Acad Sci U S A 1998;95(7);3495-500. PMID: 9520394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Wilkins98: Wilkins MR, Gasteiger E, Tonella L, Ou K, Tyler M, Sanchez JC, Gooley AA, Walsh BJ, Bairoch A, Appel RD, Williams KL, Hochstrasser DF (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278(3);599-608. PMID: 9600841

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Zimmer00: Zimmer DP, Soupene E, Lee HL, Wendisch VF, Khodursky AB, Peter BJ, Bender RA, Kustu S (2000). "Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation." Proc Natl Acad Sci U S A 97(26);14674-9. PMID: 11121068

Other References Related to Gene Regulation

Barrios99: Barrios H, Valderrama B, Morett E (1999). "Compilation and analysis of sigma(54)-dependent promoter sequences." Nucleic Acids Res 27(22);4305-13. PMID: 10536136

Caldara06: Caldara M, Charlier D, Cunin R (2006). "The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation." Microbiology 152(Pt 11);3343-54. PMID: 17074904

Caldara07: Caldara M, Minh PN, Bostoen S, Massant J, Charlier D (2007). "ArgR-dependent Repression of Arginine and Histidine Transport Genes in Escherichia coli K-12." J Mol Biol 373(2);251-67. PMID: 17850814

Makarova01: Makarova KS, Mironov AA, Gelfand MS (2001). "Conservation of the binding site for the arginine repressor in all bacterial lineages." Genome Biol 2(4);RESEARCH0013. PMID: 11305941

MedinaRivera11: Medina-Rivera A, Abreu-Goodger C, Thomas-Chollier M, Salgado H, Collado-Vides J, van Helden J (2011). "Theoretical and empirical quality assessment of transcription factor-binding motifs." Nucleic Acids Res 39(3);808-24. PMID: 20923783

Reitzer01: Reitzer L, Schneider BL (2001). "Metabolic context and possible physiological themes of sigma(54)-dependent genes in Escherichia coli." Microbiol Mol Biol Rev 65(3);422-44, table of contents. PMID: 11528004

Schmitz87: Schmitz G, Durre P, Mullenbach G, Ames GF (1987). "Nitrogen regulation of transport operons: analysis of promoters argTr and dhuA." Mol Gen Genet 209(2);403-7. PMID: 3118148

WhiteZiegler07: White-Ziegler CA, Malhowski AJ, Young S (2007). "Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12." J Bacteriol 189(15);5429-40. PMID: 17526711

Zaslaver06: Zaslaver A, Bren A, Ronen M, Itzkovitz S, Kikoin I, Shavit S, Liebermeister W, Surette MG, Alon U (2006). "A comprehensive library of fluorescent transcriptional reporters for Escherichia coli." Nat Methods 3(8);623-8. PMID: 16862137

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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