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Escherichia coli K-12 substr. MG1655 Polypeptide: lysine/arginine/ornithine ABC transporter / histidine ABC transporter - membrane subunit



Gene: hisM Accession Numbers: EG10007 (EcoCyc), b2307, ECK2301

Regulation Summary Diagram: ?

Component of:
lysine / arginine / ornithine ABC transporter (extended summary available)
histidine ABC transporter (extended summary available)

Gene Citations: [Zimmer00]

Locations: inner membrane

Map Position: [2,422,539 <- 2,423,255] (52.21 centisomes)
Length: 717 bp / 238 aa

Molecular Weight of Polypeptide: 26.87 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007613 , CGSC:25879 , EchoBASE:EB0007 , EcoGene:EG10007 , EcoliWiki:b2307 , OU-Microarray:b2307 , PortEco:hisM , PR:PRO_000022900 , Protein Model Portal:P0AEU3 , RefSeq:NP_416810 , RegulonDB:EG10007 , String:511145.b2307 , UniProt:P0AEU3

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR010065 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

In Paralogous Gene Group: 444 (2 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Zhang07, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids arginine
metabolism biosynthesis of building blocks amino acids lysine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for hisM knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: lysine / arginine / ornithine ABC transporter

Subunit composition of lysine / arginine / ornithine ABC transporter = [HisQ][HisP]2[HisM][ArgT]
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisQ
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - ATP binding subunit = HisP
         lysine/arginine/ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisM
         lysine / arginine / ornithine ABC transporter - periplasmic binding protein = ArgT

Summary:
hisP and hisMQ encode the ATP-binding component and two integral membrane components, respectively, of both the lysine/arginine/ornithine ABC transporter and the histidine ABC transporter of E. coli.

argT is a homolog of argT of Salmonella typhimurium which encodes a lysing/arginine/ornithine periplasmic binding protein [Nonet87]. The substrate specificities of ArgT and HisJ, the periplasmic histidine-binding protein, are believed to have diverged after a gene duplication event while the reliance upon HisPMQ for transport has been conserved [Higgins81].

Expression of argT is decreased after four hours of Zn(II) stress [Easton06]. argT is also repressed in response to stationary phase supernatant of autoinducer-2 deficient cells, suggesting another quorum sensing system is involved in controlling argT expression [Ren04b]. In clpP and clpA protease mutants, late stationary phase induction of argT does not occur [Weichart03]. Expression increased in early response to glucose limitation [Wick01], after ten hours of nitrogen starvation [Kabir04a], and in a glnL(Up) mutant in which NtrC-activated genes are overexpressed compared to a ntrC null mutant [Zimmer00].


Enzymatic reaction of: transport of L-ornithine (lysine / arginine / ornithine ABC transporter)


Enzymatic reaction of: transport of L-arginine (lysine / arginine / ornithine ABC transporter)


Enzymatic reaction of: transport of L-lysine (lysine / arginine / ornithine ABC transporter)


Subunit of: histidine ABC transporter

Subunit composition of histidine ABC transporter = [HisP]2[HisM][HisQ][HisJ]
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - ATP binding subunit = HisP
         lysine/arginine/ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisM
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisQ
         histidine ABC transporter - periplasmic binding protein = HisJ

Summary:
HisPMQJ is an ATP-dependent histidine transport system that is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, HisJ is the periplasmic histidine-binding protein, HisQ and HisM are the integral membrane components, and HisP is the ATP-binding component of the ABC transport complex [Liu98e]. Although the transport properties of the His complex in E. coli have not yet been characterized, extensive investigations on the orthologous proteins in Salmonella typhimurium have been reported. The His complex of Salmonella typhimurium was purified and reconstituted into ATP-encapsulated proteoliposomes, and histidine transport activity was observed [Liu97c]. His-mediated transport activity is completely dependent on the presence of all four protein components and on the internal ATP concentration, with apparent Km of 8 mM for ATP [Liu97c, Bishop89]. The transport activity is also affected by pH, temperature, and salt concentration [Liu97c]. Transport is irreversible and accumulation reaches a plateau at which point transport ceases [Liu97c]. The transport complex is inhibited by ADP and by high concentrations of internal histidine [Liu97c].


Enzymatic reaction of: histidine ABC transporter


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 23 -> 221
[UniProt09]
UniProt: ABC transmembrane type-1;
Transmembrane-Region 27 -> 47
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 59 -> 79
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 105 -> 125
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 158 -> 178
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 201 -> 221
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b2307 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10007; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bishop89: Bishop L, Agbayani R, Ambudkar SV, Maloney PC, Ames GF (1989). "Reconstitution of a bacterial periplasmic permease in proteoliposomes and demonstration of ATP hydrolysis concomitant with transport." Proc Natl Acad Sci U S A 1989;86(18);6953-7. PMID: 2674940

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Easton06: Easton JA, Thompson P, Crowder MW (2006). "Time-dependent translational response of E. coli to excess Zn(II)." J Biomol Tech 17(5);303-7. PMID: 17122063

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Higgins81: Higgins CF, Ames GF (1981). "Two periplasmic transport proteins which interact with a common membrane receptor show extensive homology: complete nucleotide sequences." Proc Natl Acad Sci U S A 78(10);6038-42. PMID: 6273842

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kabir04a: Kabir MS, Sagara T, Oshima T, Kawagoe Y, Mori H, Tsunedomi R, Yamada M (2004). "Effects of mutations in the rpoS gene on cell viability and global gene expression under nitrogen starvation in Escherichia coli." Microbiology 150(Pt 8);2543-53. PMID: 15289551

Liu97c: Liu CE, Ames GF (1997). "Characterization of transport through the periplasmic histidine permease using proteoliposomes reconstituted by dialysis." J Biol Chem 1997;272(2);859-66. PMID: 8995374

Liu98e: Liu PQ, Ames GF (1998). "In vitro disassembly and reassembly of an ABC transporter, the histidine permease." Proc Natl Acad Sci U S A 1998;95(7);3495-500. PMID: 9520394

Nonet87: Nonet ML, Marvel CC, Tolan DR (1987). "The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons." J Biol Chem 262(25);12209-17. PMID: 3040734

Ren04b: Ren D, Bedzyk LA, Ye RW, Thomas SM, Wood TK (2004). "Stationary-phase quorum-sensing signals affect autoinducer-2 and gene expression in Escherichia coli." Appl Environ Microbiol 70(4);2038-43. PMID: 15066794

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Weichart03: Weichart D, Querfurth N, Dreger M, Hengge-Aronis R (2003). "Global role for ClpP-containing proteases in stationary-phase adaptation of Escherichia coli." J Bacteriol 185(1);115-25. PMID: 12486047

Wick01: Wick LM, Quadroni M, Egli T (2001). "Short- and long-term changes in proteome composition and kinetic properties in a culture of Escherichia coli during transition from glucose-excess to glucose-limited growth conditions in continuous culture and vice versa." Environ Microbiol 3(9);588-99. PMID: 11683869

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Zimmer00: Zimmer DP, Soupene E, Lee HL, Wendisch VF, Khodursky AB, Peter BJ, Bender RA, Kustu S (2000). "Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation." Proc Natl Acad Sci U S A 97(26);14674-9. PMID: 11121068

Other References Related to Gene Regulation

Barrios99: Barrios H, Valderrama B, Morett E (1999). "Compilation and analysis of sigma(54)-dependent promoter sequences." Nucleic Acids Res 27(22);4305-13. PMID: 10536136

Caldara06: Caldara M, Charlier D, Cunin R (2006). "The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation." Microbiology 152(Pt 11);3343-54. PMID: 17074904

Caldara07: Caldara M, Minh PN, Bostoen S, Massant J, Charlier D (2007). "ArgR-dependent Repression of Arginine and Histidine Transport Genes in Escherichia coli K-12." J Mol Biol 373(2);251-67. PMID: 17850814

Makarova01: Makarova KS, Mironov AA, Gelfand MS (2001). "Conservation of the binding site for the arginine repressor in all bacterial lineages." Genome Biol 2(4);RESEARCH0013. PMID: 11305941

MedinaRivera11: Medina-Rivera A, Abreu-Goodger C, Thomas-Chollier M, Salgado H, Collado-Vides J, van Helden J (2011). "Theoretical and empirical quality assessment of transcription factor-binding motifs." Nucleic Acids Res 39(3);808-24. PMID: 20923783

Reitzer01: Reitzer L, Schneider BL (2001). "Metabolic context and possible physiological themes of sigma(54)-dependent genes in Escherichia coli." Microbiol Mol Biol Rev 65(3);422-44, table of contents. PMID: 11528004

Schmitz87: Schmitz G, Durre P, Mullenbach G, Ames GF (1987). "Nitrogen regulation of transport operons: analysis of promoters argTr and dhuA." Mol Gen Genet 209(2);403-7. PMID: 3118148

WhiteZiegler07: White-Ziegler CA, Malhowski AJ, Young S (2007). "Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12." J Bacteriol 189(15);5429-40. PMID: 17526711

Zaslaver06: Zaslaver A, Bren A, Ronen M, Itzkovitz S, Kikoin I, Shavit S, Liebermeister W, Surette MG, Alon U (2006). "A comprehensive library of fluorescent transcriptional reporters for Escherichia coli." Nat Methods 3(8);623-8. PMID: 16862137


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 21, 2014, biocyc13.