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Escherichia coli K-12 substr. MG1655 Transporter: 2-O-α-mannosyl-D-glycerate PTS permease



Gene: mngA Accession Numbers: EG13235 (EcoCyc), b0731, ECK0719

Synonyms: frx, hrsA, EIIMngA, EIIHrsA, EIImannosylglycerate, Enzyme IImannosylglycerate

Regulation Summary Diagram: ?

Summary:
MngA, a PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its substrates in a process called group translocation (reviewed in [Postma93]. MngA takes up exogenous 2-O-α-mannosyl-D-glycerate (MG), releasing the phosphate ester into the cell cytoplasm for hydrolysis by the mngB encoded α-mannosidase [Sampaio04].

E. coli K-12 can use MG as the sole source of carbon. An E. coli ΔmngA strain cannot grow with MG as sole carbon source. MG induces expression of mngA. Membranes containing MngA are able to phosphorylate MG in vitro in the presence of PEP, PtsI and PtsH [Sampaio04].

mngA formerly known as hsrA was initially identified during a study of thermoresponsive genes in E. coli K-12 [Utsumi96]. mngA contains IIA, IIB and IIC PTS domains in a single polypeptide chain and shows sequence similarity with the fructose specific PTS proteins. MngA contains two putative phosphorylation sites at His87 and Cys192 [Utsumi96, Reizer]. MngA is a member of the PTS Fructose-Mannitol (Fru) family of transporters [Saier14].

The overall PTS-mediated phosphoryl transfer reaction, requiring the two general energy coupling proteins of the PTS, Enzyme I (PtsI) and HPr (PtsH), as well as the three domains of the Enzyme IIMngA complex can be represented as:

PEP → Enzyme I-Phis189 → HPr-Phis15 → Enzyme IIA-Phis87 → Enzyme IIB-Pcys192-(Enzyme IIC) → 2-O-6-phospho-α-mannosyl-D-glycerate

Locations: inner membrane

Map Position: [765,207 -> 767,183] (16.49 centisomes)
Length: 1977 bp / 658 aa

Molecular Weight of Polypeptide: 69.668 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002493 , EchoBASE:EB3024 , EcoGene:EG13235 , OU-Microarray:b0731 , PortEco:mngA , RegulonDB:EG13235

Relationship Links: EcoO157Cyc:Homolog:FRWC_2

In Paralogous Gene Group: 187 (14 members) , 188 (6 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from experiment [Sampaio04]
GO:0051476 - mannosylglycerate transport Inferred from experiment [Sampaio04]
Molecular Function: GO:0090581 - protein-phosphocysteine-mannosylglycerate-phosphotransferase system transporter activity Inferred from experiment [Sampaio04]
GO:0090563 - protein-phosphocysteine-sugar phosphotransferase activity Inferred by computational analysis [Utsumi96]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [DiazMejia09, Daley05, Sampaio04]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Utsumi96]

MultiFun Terms: cell processes adaptations temperature extremes
cell structure membrane
information transfer protein related posttranslational modification
metabolism carbon utilization carbon compounds
transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for mngA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: 2-O-α-mannosyl-D-glycerate PTS permease

Synonyms: Transport of 2-O-α-mannosyl-D-glycerate

Kinetic Parameters:

Substrate
Km (μM)
Vmax (µmol mg-1 min-1)
Citations
2-O-α-mannosyl-D-glycerate
10.0
[Sampaio04]
2-O-α-mannosyl-D-glycerate
6.5e-4


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 25 -> 171
[UniProt09]
UniProt: PTS EIIA type-2;
Active-Site 87
[UniProt10]
UniProt: Tele-phosphohistidine intermediate; for EIIA activity; Non-Experimental Qualifier: by similarity;
Conserved-Region 186 -> 282
[UniProt09]
UniProt: PTS EIIB type-2;
Active-Site 192
[UniProt10]
UniProt: Phosphocysteine intermediate; for EIIB activity; Non-Experimental Qualifier: by similarity;
Conserved-Region 306 -> 641
[UniProt09]
UniProt: PTS EIIC type-2;
Transmembrane-Region 314 -> 334
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 359 -> 379
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 390 -> 410
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 434 -> 454
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 475 -> 495
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 501 -> 521
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 552 -> 572
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 574 -> 594
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 621 -> 641
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0731 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG13235; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer: Reizer J, Reizer A "A voyage along the bases: novel phosphotransferase genes revealed by in silico analyses of the Escherichia coli genome." Res Microbiol 147(6-7);458-71. PMID: 9092011

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

Sampaio04: Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO (2004). "Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor." J Biol Chem 279(7);5537-48. PMID: 14645248

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

Utsumi96: Utsumi R, Horie T, Katoh A, Kaino Y, Tanabe H, Noda M (1996). "Isolation and characterization of the heat-responsive genes in Escherichia coli." Biosci Biotechnol Biochem 60(2);309-15. PMID: 9063979

Other References Related to Gene Regulation

GamaCastro08: Gama-Castro S, Jimenez-Jacinto V, Peralta-Gil M, Santos-Zavaleta A, Penaloza-Spinola MI, Contreras-Moreira B, Segura-Salazar J, Muniz-Rascado L, Martinez-Flores I, Salgado H, Bonavides-Martinez C, Abreu-Goodger C, Rodriguez-Penagos C, Miranda-Rios J, Morett E, Merino E, Huerta AM, Trevino-Quintanilla L, Collado-Vides J (2008). "RegulonDB (version 6.0): gene regulation model of Escherichia coli K-12 beyond transcription, active (experimental) annotated promoters and Textpresso navigation." Nucleic Acids Res 36(Database issue);D120-4. PMID: 18158297

Quail94a: Quail MA, Dempsey CE, Guest JR (1994). "Identification of a fatty acyl responsive regulator (FarR) in Escherichia coli." FEBS Lett 1994;356(2-3);183-7. PMID: 7805834


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.