Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015

Escherichia coli K-12 substr. MG1655 Protein: lipoyl-ACP

Gene: acpP Accession Numbers: EG50003 (EcoCyc), b1094, ECK1080

Regulation Summary Diagram: ?

Alternative forms of lipoyl-ACP:
apo-[acyl carrier protein] (summary available)
octanoyl-ACP (summary available)
holo-[acyl-carrier protein] (summary available)

Lipoate synthase catalyzes the step of lipoic acid biosynthesis at which sulfur is inserted into octanoyl-ACP to form the lipoate moiety [Reed93, Miller00a]. Lipoate modification (by lipoyl-protein ligases LipB or LplA) of complex subunits is important for function of pyruvate dehydrogenase [Herbert75, Stepp81, Reed93], alpha-ketoglutarate dehydrogenase [Herbert75, Stepp81, Reed93], and the glycine cleavage system [Vanden91, Reed93].

Gene Citations: [Zhang96, Rawlings92]

Map Position: [1,150,838 -> 1,151,074] (24.8 centisomes)
Length: 237 bp / 78 aa

Molecular Weight: 8.6395 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003703 , CGSC:31871 , EchoBASE:EB4297 , EcoGene:EG50003 , OU-Microarray:b1094 , PortEco:acpP , RefSeq:NP_415612 , RegulonDB:EG50003

Relationship Links: Pfam:IN-FAMILY:PF00550

Reactions known to consume the compound:

CDP-diacylglycerol biosynthesis II :
a 1-acyl-sn-glycerol 3-phosphate + an acyl-[acyl-carrier protein] → a 1,2-diacyl-sn-glycerol 3-phosphate + a holo-[acyl-carrier protein]
sn-glycerol 3-phosphate + an acyl-[acyl-carrier protein] → a 1-acyl-sn-glycerol 3-phosphate + a holo-[acyl-carrier protein]

Reactions known to both consume and produce the compound:

Not in pathways:
an acyl-[acyl-carrier protein] + a 1-lyso-2-acyl-sn-glycero-3-phosphoethanolamine ↔ a holo-[acyl-carrier protein] + an L-1-phosphatidyl-ethanolamine

In Reactions of unknown directionality:

Not in pathways:
SucB + lipoyl-ACP = SucB-lipoate + a holo-[acyl-carrier protein]
AceF + lipoyl-ACP = AceF-lipoate + a holo-[acyl-carrier protein]

[glycine cleavage system lipoyl-carrier protein]-L-lysine + a lipoyl-[acp] = a [glycine-cleavage complex H protein] N6-lipoyl-L-lysine + a holo-[acyl-carrier protein] + H+

an acyl-[acyl-carrier protein] + NAD+ = a trans-2-enoyl-[acyl-carrier protein] + NADH + H+
an acyl-[acyl-carrier protein] + a malonyl-[acp] + H+ = a 3-oxoacyl-[acp] + CO2 + a holo-[acyl-carrier protein]

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

MultiFun Terms: metabolism biosynthesis of macromolecules (cellular constituents) large molecule carriers acyl carrier protein

Gene Local Context (not to scale): ?

Transcription Units:


10/20/97 Gene b1094 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG50003; confirmed by SwissProt match.


Herbert75: Herbert AA, Guest JR (1975). "Lipoic acid content of Escherichia coli and other microorganisms." Arch Microbiol 106(3);259-66. PMID: 814874

Miller00a: Miller JR, Busby RW, Jordan SW, Cheek J, Henshaw TF, Ashley GW, Broderick JB, Cronan JE, Marletta MA (2000). "Escherichia coli LipA is a lipoyl synthase: in vitro biosynthesis of lipoylated pyruvate dehydrogenase complex from octanoyl-acyl carrier protein." Biochemistry 39(49);15166-78. PMID: 11106496

Rawlings92: Rawlings M, Cronan JE (1992). "The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes." J Biol Chem 1992;267(9);5751-4. PMID: 1556094

Reed93: Reed KE, Cronan JE (1993). "Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes." J Bacteriol 175(5);1325-36. PMID: 8444795

Stepp81: Stepp LR, Bleile DM, McRorie DK, Pettit FH, Reed LJ (1981). "Use of trypsin and lipoamidase to study the role of lipoic acid moieties in the pyruvate and alpha-ketoglutarate dehydrogenase complexes of Escherichia coli." Biochemistry 20(16);4555-60. PMID: 6794598

Vanden91: Vanden Boom TJ, Reed KE, Cronan JE (1991). "Lipoic acid metabolism in Escherichia coli: isolation of null mutants defective in lipoic acid biosynthesis, molecular cloning and characterization of the E. coli lip locus, and identification of the lipoylated protein of the glycine cleavage system." J Bacteriol 173(20);6411-20. PMID: 1655709

Zhang96: Zhang Y, Cronan JE (1996). "Polar allele duplication for transcriptional analysis of consecutive essential genes: application to a cluster of Escherichia coli fatty acid biosynthetic genes." J Bacteriol 1996;178(12);3614-20. PMID: 8655562

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Mar 4, 2015, biocyc14.