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Escherichia coli K-12 substr. MG1655 Protein: lipoyl-ACP



Gene: acpP Accession Numbers: EG50003 (EcoCyc), b1094, ECK1080

Regulation Summary Diagram: ?

Regulation summary diagram for acpP

Alternative forms of lipoyl-ACP:
apo-[acyl carrier protein] (summary available)
octanoyl-ACP (summary available)
succinyl-ACP (summary available)
cis3-decenoyl-ACP (summary available)
trans-hexadec-2-enoyl-ACP (summary available)
(3R)-3-hydroxyoctanoyl-ACP (summary available)
(3R)-hydroxy cis Δ5-dodecenoyl-ACP (summary available)
3-oxo-cis5-dodecenoyl-ACP (summary available)
trans-but-2-enoyl-ACP (summary available)
cis5-dodecenoyl-ACP (summary available)
trans oct-2-enoyl-ACP (summary available)
(3R)-3-hydroxybutanoyl-ACP (summary available)
(3R)-3-hydroxypalmitoyl-ACP (summary available)
trans3-cis-Δ5-dodecenoyl-ACP (summary available)
holo-[acyl-carrier protein] (summary available)

Summary:
Acyl carrier protein (ACP) plays an important role in fatty acid biosynthesis. It carries fatty acid chains via a thioester linkage to a 4'-phosphopantetheine prosthetic group as the chains are elongated. There is also evidence that it has a function in the biosynthesis of membrane-derived oligosaccharides. ACP and its acylated forms interact with at least 12 different Escherichia coli enzymes [Rawlings92, Holak88, Holak88a].

ACP is the most abundant protein in Escherichia coli, with about 1.5 million molecules per cell [Mathews90]. ACP contains a 4'-phosphopantetheine moiety (as does Coenzyme A) as the reactive unit, attached to the ACP protein through a serine side chain. The holo-[acyl-carrier-protein] synthase enzyme (encoded by acpS) transfers the 4'-phosphopantetheine moiety of coenzyme A to the apo-[acyl carrier protein] to form holo-[acyl-carrier protein], which is the active form of the carrier in lipid synthesis [Elovson68, Polacco81].

Gene Citations: [Zhang96a]

Map Position: [1,150,838 -> 1,151,074] (24.8 centisomes, 89°)
Length: 237 bp / 78 aa

Molecular Weight: 8.6395 kD (from nucleotide sequence)

lipoyl-ACP compound structure

Unification Links: ASAP:ABE-0003703 , CGSC:31871 , EchoBASE:EB4297 , EcoGene:EG50003 , OU-Microarray:b1094 , PortEco:acpP , RefSeq:NP_415612 , RegulonDB:EG50003

Relationship Links: Pfam:IN-FAMILY:PF00550

Reactions known to consume the compound:

CDP-diacylglycerol biosynthesis II :
a 1-acyl-sn-glycerol 3-phosphate + an acyl-[acyl-carrier protein] → a 1,2-diacyl-sn-glycerol 3-phosphate + a holo-[acyl-carrier protein]
sn-glycerol 3-phosphate + an acyl-[acyl-carrier protein] → a 1-acyl-sn-glycerol 3-phosphate + a holo-[acyl-carrier protein]

Reactions known to both consume and produce the compound:

Not in pathways:
an acyl-[acyl-carrier protein] + a 2-acyl-1-lyso-phosphatidylethanolamine ↔ a holo-[acyl-carrier protein] + an L-1-phosphatidylethanolamine

In Reactions of unknown directionality:

Not in pathways:
SucB + lipoyl-ACP = SucB-lipoate + a holo-[acyl-carrier protein]
AceF + lipoyl-ACP = AceF-lipoate + a holo-[acyl-carrier protein]

Not in pathways:
[glycine cleavage system lipoyl-carrier protein]-L-lysine + a lipoyl-[acp] = a [glycine-cleavage complex H protein] N6-lipoyl-L-lysine + a holo-[acyl-carrier protein] + H+

Not in pathways:
an acyl-[acyl-carrier protein] + NAD+ = a trans-2-enoyl-[acyl-carrier protein] + NADH + H+
an acyl-[acyl-carrier protein] + a malonyl-[acp] + H+ = a 3-oxoacyl-[acp] + CO2 + a holo-[acyl-carrier protein]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for acpP

GO Terms:

Biological Process: GO:0042967 - acyl-carrier-protein biosynthetic process

MultiFun Terms: metabolism biosynthesis of macromolecules (cellular constituents) large molecule carriers acyl carrier protein


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Notes:

History:
10/20/97 Gene b1094 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG50003; confirmed by SwissProt match.


References

Elovson68: Elovson J, Vagelos PR (1968). "Acyl carrier protein. X. Acyl carrier protein synthetase." J Biol Chem 1968;243(13);3603-11. PMID: 4872726

Holak88: Holak TA, Kearsley SK, Kim Y, Prestegard JH (1988). "Three-dimensional structure of acyl carrier protein determined by NMR pseudoenergy and distance geometry calculations." Biochemistry 1988;27(16);6135-42. PMID: 3056520

Holak88a: Holak TA, Nilges M, Prestegard JH, Gronenborn AM, Clore GM (1988). "Three-dimensional structure of acyl carrier protein in solution determined by nuclear magnetic resonance and the combined use of dynamical simulated annealing and distance geometry." Eur J Biochem 1988;175(1);9-15. PMID: 3402450

Mathews90: Mathews CK, van Holde KE (1990). "Biochemistry." The Benjamin/Cummings Publishing Company, Inc., Redwood City.

Polacco81: Polacco ML, Cronan JE (1981). "A mutant of Escherichia coli conditionally defective in the synthesis of holo-[acyl carrier protein]." J Biol Chem 1981;256(11);5750-4. PMID: 7016860

Rawlings92: Rawlings M, Cronan JE (1992). "The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes." J Biol Chem 1992;267(9);5751-4. PMID: 1556094

Zhang96a: Zhang Y, Cronan JE (1996). "Polar allele duplication for transcriptional analysis of consecutive essential genes: application to a cluster of Escherichia coli fatty acid biosynthetic genes." J Bacteriol 1996;178(12);3614-20. PMID: 8655562


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Aug 28, 2015, BIOCYC14A.