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Escherichia coli K-12 substr. MG1655 Protein: lipoyl-ACP



Gene: acpP Accession Numbers: EG50003 (EcoCyc), b1094, ECK1080

Regulation Summary Diagram: ?

Alternative forms of lipoyl-ACP:
apo-[acyl carrier protein] (summary available)
octanoyl-ACP (summary available)
succ-ACP
cis-Δ3-decenoyl-ACP
2-hexadecenoyl-ACP
cis-3,4-dehydrodecanoyl-ACP
(3R)-3-hydroxyoctanoyl-ACP
β-hydroxy-cis-Δ5-dodecenoyl-ACP
β-keto-cis-Δ5-dodecenoyl-ACP
but-2-enoyl-ACP
cis-Δ5-dodecenoyl-ACP
2-octenoyl-ACP
3R-3-hydroxybutanoyl-ACP
R-3-hydroxypalmitoyl-ACP
trans-Δ3-cis-Δ5-dodecenoyl-ACP
holo-[acyl-carrier protein] (summary available)

Summary:
Lipoate synthase catalyzes the step of lipoic acid biosynthesis at which sulfur is inserted into octanoyl-ACP to form the lipoate moiety [Reed93, Miller00a]. Lipoate modification (by lipoyl-protein ligases LipB or LplA) of complex subunits is important for function of pyruvate dehydrogenase [Herbert75, Stepp81, Reed93], alpha-ketoglutarate dehydrogenase [Herbert75, Stepp81, Reed93], and the glycine cleavage system [Vanden91, Reed93].

Gene Citations: [Zhang96, Rawlings92]

Map Position: [1,150,838 -> 1,151,074] (24.8 centisomes)
Length: 237 bp / 78 aa

Molecular Weight: 8.6395 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003703 , CGSC:31871 , EchoBASE:EB4297 , EcoGene:EG50003 , OU-Microarray:b1094 , PortEco:acpP , RefSeq:NP_415612 , RegulonDB:EG50003

Relationship Links: Pfam:IN-FAMILY:PF00550

Reactions known to consume the compound:

CDP-diacylglycerol biosynthesis II :
a 1-acyl-sn-glycerol 3-phosphate + an acyl-[acyl-carrier protein] → a 1,2-diacyl-sn-glycerol 3-phosphate + a holo-[acyl-carrier protein]
sn-glycerol 3-phosphate + an acyl-[acyl-carrier protein] → a 1-acyl-sn-glycerol 3-phosphate + a holo-[acyl-carrier protein]

Reactions known to both consume and produce the compound:

Not in pathways:
an acyl-[acyl-carrier protein] + a 1-lyso-2-acyl-sn-glycero-3-phosphoethanolamine ↔ a holo-[acyl-carrier protein] + an L-1-phosphatidyl-ethanolamine

In Reactions of unknown directionality:

Not in pathways:
SucB + lipoyl-ACP = SucB-lipoate + a holo-[acyl-carrier protein]
AceF + lipoyl-ACP = AceF-lipoate + a holo-[acyl-carrier protein]


[glycine cleavage system lipoyl-carrier protein]-L-lysine + a lipoyl-[acp] = a [glycine-cleavage complex H protein] N6-lipoyl-L-lysine + a holo-[acyl-carrier protein] + H+


an acyl-[acyl-carrier protein] + NAD+ = a trans-2-enoyl-[acyl-carrier protein] + NADH + H+
an acyl-[acyl-carrier protein] + a malonyl-[acp] + H+ = a 3-oxoacyl-[acp] + CO2 + a holo-[acyl-carrier protein]

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

MultiFun Terms: metabolism biosynthesis of macromolecules (cellular constituents) large molecule carriers acyl carrier protein


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b1094 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG50003; confirmed by SwissProt match.


References

Herbert75: Herbert AA, Guest JR (1975). "Lipoic acid content of Escherichia coli and other microorganisms." Arch Microbiol 106(3);259-66. PMID: 814874

Miller00a: Miller JR, Busby RW, Jordan SW, Cheek J, Henshaw TF, Ashley GW, Broderick JB, Cronan JE, Marletta MA (2000). "Escherichia coli LipA is a lipoyl synthase: in vitro biosynthesis of lipoylated pyruvate dehydrogenase complex from octanoyl-acyl carrier protein." Biochemistry 39(49);15166-78. PMID: 11106496

Rawlings92: Rawlings M, Cronan JE (1992). "The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes." J Biol Chem 1992;267(9);5751-4. PMID: 1556094

Reed93: Reed KE, Cronan JE (1993). "Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes." J Bacteriol 175(5);1325-36. PMID: 8444795

Stepp81: Stepp LR, Bleile DM, McRorie DK, Pettit FH, Reed LJ (1981). "Use of trypsin and lipoamidase to study the role of lipoic acid moieties in the pyruvate and alpha-ketoglutarate dehydrogenase complexes of Escherichia coli." Biochemistry 20(16);4555-60. PMID: 6794598

Vanden91: Vanden Boom TJ, Reed KE, Cronan JE (1991). "Lipoic acid metabolism in Escherichia coli: isolation of null mutants defective in lipoic acid biosynthesis, molecular cloning and characterization of the E. coli lip locus, and identification of the lipoylated protein of the glycine cleavage system." J Bacteriol 173(20);6411-20. PMID: 1655709

Zhang96: Zhang Y, Cronan JE (1996). "Polar allele duplication for transcriptional analysis of consecutive essential genes: application to a cluster of Escherichia coli fatty acid biosynthetic genes." J Bacteriol 1996;178(12);3614-20. PMID: 8655562


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc12.