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Escherichia coli K-12 substr. MG1655 Enzyme: D-mannonate oxidoreductase



Gene: uxuB Accession Numbers: EG20248 (EcoCyc), b4323, ECK4314

Regulation Summary Diagram: ?

Summary:
D-mannonate oxidoreductase is an enzyme of the D-glucuronate degradation pathway, reducing D-fructuronate to D-mannonate.

The reaction proceeds via a rapid-equilibrium random bi-bi + dead-end EBQ complex mechanism [Portalier72a]. Reports differ on whether [MandrandBerthel77] or not [Portalier72] the enzyme is able to use D-glucuronate as a substrate. Where it was measured, the reaction with D-glucuronate appears to proceed via a different mechanism [MandrandBerthel77].

The enzyme is induced by both glucuronate and fructuronate [Portalier72]; the internal inducer is fructuronate [RobertBaudouy74].

Gene Citations: [Blanco86]

Locations: cytosol

Map Position: [4,550,924 -> 4,552,384] (98.09 centisomes)
Length: 1461 bp / 486 aa

Molecular Weight of Polypeptide: 53.58 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0014175 , CGSC:13 , DIP:DIP-11109N , EchoBASE:EB4150 , EcoGene:EG20248 , EcoliWiki:b4323 , ModBase:P39160 , OU-Microarray:b4323 , PortEco:uxuB , PR:PRO_000024207 , Pride:P39160 , Protein Model Portal:P39160 , RefSeq:NP_418743 , RegulonDB:EG20248 , SMR:P39160 , String:511145.b4323 , Swiss-Model:P39160 , UniProt:P39160

Relationship Links: InterPro:IN-FAMILY:IPR000669 , InterPro:IN-FAMILY:IPR008927 , InterPro:IN-FAMILY:IPR013118 , InterPro:IN-FAMILY:IPR013131 , InterPro:IN-FAMILY:IPR013328 , InterPro:IN-FAMILY:IPR016040 , InterPro:IN-FAMILY:IPR023027 , Pfam:IN-FAMILY:PF01232 , Pfam:IN-FAMILY:PF08125 , Prints:IN-FAMILY:PR00084 , Prosite:IN-FAMILY:PS00974

In Paralogous Gene Group: 306 (5 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0042840 - D-glucuronate catabolic process Inferred from experiment [Ritzenthaler80]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
GO:0019594 - mannitol metabolic process Inferred by computational analysis [GOA01]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0008866 - fructuronate reductase activity Inferred from experiment Inferred by computational analysis [GOA01a, Ritzenthaler80, Portalier72]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016616 - oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred by computational analysis [GOA01]
GO:0050662 - coenzyme binding Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds

Essentiality data for uxuB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Last-Curated ? 01-Feb-2008 by Keseler I , SRI International


Enzymatic reaction of: D-mannonate oxidoreductase

Synonyms: fructuronate reductase, D-mannonate:NAD+ 5-oxidoreductase, D-mannonic dehydrogenase

EC Number: 1.1.1.57

D-mannonate + NAD+ <=> D-fructuronate + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Portalier72]

Alternative Substrates for NADH: NADPH [Portalier72 ]

Alternative Substrates for D-mannonate: D-altronate [Portalier72 , Hickman60 ]

Alternative Substrates for D-fructuronate: D-tagaturonate [Linster04 , Hickman60 , MandrandBerthel77 , Portalier72 ] , D-glucuronate [MandrandBerthel77 ]

In Pathways: superpathway of hexuronide and hexuronate degradation , superpathway of β-D-glucuronide and D-glucuronate degradation , D-fructuronate degradation

Summary:
The optimal pH for fructuronate reduction is 6.3, while the optimal pH for the reverse reaction, oxidation of mannonate, is 8.4. NADPH can substitute for NADH; the Km is 700 µM [Portalier72].

Activators (Unknown Mechanism): Mg2+ [Portalier72] , Ca2+ [Portalier72] , Mn2+ [Portalier72] , Zn2+ [Portalier72]

Inhibitors (Competitive): ATP [MandrandBerthel77] , NAD+ [Portalier72a, MandrandBerthel77]

Inhibitors (Noncompetitive): p-chloromercuribenzoate [Hickman60, Portalier72]

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-fructuronate
1000.0
[Hickman60, BRENDA14]
D-fructuronate
500.0
[Portalier82, BRENDA14]
D-fructuronate
500.0
[Portalier72, BRENDA14]
NADH
30.0
[Portalier72]
D-mannonate
1700.0
[Portalier72b, BRENDA14]
D-mannonate
1800.0
[Portalier72, BRENDA14]
D-mannonate
780.0
[Portalier82, BRENDA14]
D-mannonate
780.0
[Portalier72a, BRENDA14]
NAD+
200.0
[Portalier72]

pH(opt): 6 [BRENDA14, Hickman60], 8.4 [BRENDA14, Portalier82], 6.3 [Portalier72]


Sequence Features

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 25 -> 36
[UniProt10a]
UniProt: NAD; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b4323 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG20248; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Blanco86: Blanco C, Ritzenthaler P, Kolb A (1986). "The regulatory region of the uxuAB operon in Escherichia coli K12." Mol Gen Genet 1986;202(1);112-9. PMID: 3083215

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Hickman60: Hickman J, Ashwell G (1960). "Uronic acid metabolism in bacteria. II. Purification and properties of D-altronic acid and D-mannonic acid dehydrogenases in Escherichia coli." J Biol Chem 235;1566-70. PMID: 14401695

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Linster04: Linster CL, Van Schaftingen E (2004). "A spectrophotometric assay of D-glucuronate based on Escherichia coli uronate isomerase and mannonate dehydrogenase." Protein Expr Purif 37(2);352-60. PMID: 15358357

MandrandBerthel77: Mandrand-Berthelot MA, Lagarde AE (1977). ""Hit-and-run" mechanism for D-glucuronate reduction catalyzed by D-mannonate:NAD oxidoreductase of Escherichia coli." Biochim Biophys Acta 1977;483(1);6-23. PMID: 195622

Portalier72: Portalier RC, Stoeber FR (1972). "[D-mannonate: NAD oxidoreductase from Escherichia coli K12. Purification, properties and specificity]." Eur J Biochem 1972;26(2);290-300. PMID: 4402996

Portalier72a: Portalier RC (1972). "[D-mannonate: NAD-oxidoreductase from Escherichia coli K12. Kinetic studies of the enzymatic mechanism]." Eur J Biochem 1972;30(2);220-33. PMID: 4351435

Portalier72b: Portalier RC, Stoeber FR (1972). "[Colorimetric determinations of aldonate oxidoreductases of Escherichia coli K 12: applications]." Biochim Biophys Acta 289(1);19-27. PMID: 4564054

Portalier82: Portalier R, Stoeber F (1982). "D-Mannonate and D-altronate-NAD dehydrogenases from Escherichia coli." Methods Enzymol 89 Pt D;210-8. PMID: 6755169

Ritzenthaler80: Ritzenthaler P, Mata-Gilsinger M, Stoeber F (1980). "Construction and expression of hybrid plasmids containing Escherichia coli K-12 uxu genes." J Bacteriol 143(3);1116-26. PMID: 6997264

RobertBaudouy74: Robert-Baudouy JM, Portalier RC (1974). "[Studies of mutations in glucuronate catabolism in Escherichia coli K12 (author's transl)]." Mol Gen Genet 131(1);31-46. PMID: 4603847

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Bates04: Bates Utz C, Nguyen AB, Smalley DJ, Anderson AB, Conway T (2004). "GntP is the Escherichia coli Fructuronic acid transporter and belongs to the UxuR regulon." J Bacteriol 186(22);7690-6. PMID: 15516583

Klemm96: Klemm P, Tong S, Nielsen H, Conway T (1996). "The gntP gene of Escherichia coli involved in gluconate uptake." J Bacteriol 178(1);61-7. PMID: 8550444

Lynch96: Lynch AS, Lin EC (1996). "Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli: characterization of DNA binding at target promoters." J Bacteriol 1996;178(21);6238-49. PMID: 8892825

Ritzenthaler82: Ritzenthaler P, Mata-Gilsinger M (1982). "Use of in vitro gene fusions to study the uxuR regulatory gene in Escherichia coli K-12: direction of transcription and regulation of its expression." J Bacteriol 150(3);1040-7. PMID: 6281232

Ritzenthaler85: Ritzenthaler P, Blanco C, Mata-Gilsinger M (1985). "Genetic analysis of uxuR and exuR genes: evidence for ExuR and UxuR monomer repressors interactions." Mol Gen Genet 199(3);507-11. PMID: 3929016

RobertBaudouy81: Robert-Baudouy J, Portalier R, Stoeber F (1981). "Regulation of hexuronate system genes in Escherichia coli K-12: multiple regulation of the uxu operon by exuR and uxuR gene products." J Bacteriol 145(1);211-20. PMID: 7007313

Rodionov00: Rodionov DA, Mironov AA, Rakhmaninova AB, Gelfand MS (2000). "Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria." Mol Microbiol 38(4);673-83. PMID: 11115104

Zheng01a: Zheng M, Wang X, Templeton LJ, Smulski DR, LaRossa RA, Storz G (2001). "DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide." J Bacteriol 183(15);4562-70. PMID: 11443091

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, biocyc14.